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AB113720

Anti-PCDH12 antibody

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(2 Publications)

Rabbit Polyclonal PCDH12 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PCDH12 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

UNQ395/PRO731, PCDH12, Protocadherin-12, Vascular cadherin-2, Vascular endothelial cadherin-2, VE-cad-2, VE-cadherin-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH12 antibody (AB113720)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH12 antibody (AB113720)

Immunohistochemical staining of Human Spleen using ab113720 at 5μg/ml

Western blot - Anti-PCDH12 antibody (AB113720)
  • WB

Unknown

Western blot - Anti-PCDH12 antibody (AB113720)

All lanes:

Western blot - Anti-PCDH12 antibody (ab113720) at 2 µg/mL

All lanes:

K562 cell lysate

Predicted band size: 128 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PCDH12 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NPG4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCDH12 also known as Protocadherin 12 belongs to the protocadherin family which is part of the cadherin superfamily. PCDH12 has a molecular mass of about 120 kDa. This protein is expressed in endothelial cells and certain types of smooth muscle tissues. Researchers have identified it in the central nervous system and specific fetal tissues suggesting a role in early development and vascular formation.
Biological function summary

PCDH12 contributes to cell-cell adhesion mechanisms. It plays a significant part in maintaining cellular architecture and establishing cell connectivity. Although not definitively confirmed as part of a larger complex its function aligns with other cadherins that mediate homophilic binding to stabilize cell interactions. It also participates in the development of the blood-brain barrier by supporting endothelial junctions.

Pathways

PCDH12 functions within the signaling networks related to cell adhesion and migration. It influences angiogenesis through its role in adherens junction pathways. This involvement links it to proteins such as VE-cadherin which are critical for vascular integrity. Additionally PCDH12 interacts with pathways that manage neural development potentially cooperating with other neural-specific cadherins.

PCDH12 shows connections to cerebrovascular diseases and certain cancer types. In stroke research altered PCDH12 expression correlates with blood-brain barrier dysfunction. Studies also indicate a possible involvement in tumor progression where its regulation affects cellular proliferation pathways. PCDH12 interacts with proteins like beta-catenin which may facilitate changes in cell signaling during tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (By similarity). Acts as a regulator of cell migration, probably via increasing cell-cell adhesion (PubMed : 21402705). Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions (By similarity). Unable to bind to catenins, weakly associates with the cytoskeleton (By similarity).
See full target information PCDH12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed33141023

2020

Single nuclei RNA-seq of mouse placental labyrinth development.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan Marsh,Robert Blelloch

Glia 67:967-984 PubMed30667096

2019

The PTN-PTPRZ signal activates the AFAP1L2-dependent PI3K-AKT pathway for oligodendrocyte differentiation: Targeted inactivation of PTPRZ activity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Naomi Tanga,Kazuya Kuboyama,Ayako Kishimoto,Hiroshi Kiyonari,Aki Shiraishi,Ryoko Suzuki,Toshio Watanabe,Akihiro Fujikawa,Masaharu Noda
View all publications
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