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AB202560

Anti-PCDH15 antibody [EPR19600]

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal PCDH15 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Protocadherin-15, Pcdh15

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCDH15 antibody [EPR19600] (AB202560)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCDH15 antibody [EPR19600] (AB202560)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged mouse PCDH15 expression construct or GFP vector only labeling PCDH15 with ab202560 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

Confocal image showing positive staining on HEK-293 cells transfected with a GFP-tagged mouse PCDH15 expression construct.

This mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi Nanjing University MARC.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150115 (Alexa Fluor ® 647 Goat anti-Mouse secondary) at 1/1000 dilution (white).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 594) (ab150080) at 1/1000 dilution.

Western blot - Anti-PCDH15 antibody [EPR19600] (AB202560)
  • WB

Supplier Data

Western blot - Anti-PCDH15 antibody [EPR19600] (AB202560)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 16481439).

The mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

WB detection of endogenous PCDH15 is not shown due to unavailability of appropriate testing material.

All lanes:

Western blot - Anti-PCDH15 antibody [EPR19600] (ab202560) at 1/1000 dilution

Lane 1:

293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with an empty expression vector at 10 µg

Lane 2:

293T whole cell lysate transfected with a DDDK-tagged mouse PCDH15 construct at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 216 kDa

Observed band size: 260 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19600

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCDH15 also known as Protocadherin-15 is a protein encoded by the PCDH15 gene. Mechanically this protein functions as an adhesion molecule in the body playing an important role in cell-cell interactions. PCDH15 has a molecular mass of approximately 223 kDa. It has a widespread expression but is particularly found in sensory cells in the inner ear and in the retina of the eye highlighting its importance in sensory functions.
Biological function summary

PCDH15 participates in the formation of tip-link structures within the inner ear. This protein is a component of the mechanotransduction complex which translates mechanical stimuli into electrical signals. PCDH15 works alongside other proteins like Cadherin 23 another protocadherin to maintain the integrity of hair cells. Its role is essential for proper auditory and visual signal processing contributing to the sense of hearing and vision.

Pathways

PCDH15 interacts with the mechanotransduction pathway in sensory cells. This pathway plays a role in converting mechanical stimuli such as sound waves in the cochlea into nerve impulses. Cadherin 23 is closely related in this pathway and together these proteins maintain the hair cell structure necessary for mechanotransduction. The interplay between these proteins ensures the transduction of sensory information impacting cellular responses in sensory organs.

PCDH15 mutations associate with Usher syndrome type 1F and non-syndromic hearing loss. Usher syndrome is a genetic condition involving hearing loss and vision impairment. PCDH15 mutations disrupt the mechanotransduction complex affecting sensory perception. Cadherin 23 also connects to these disorders as mutations in this protein can similarly lead to issues in hearing and vision. Understanding PCDH15 and its interactors is critical in addressing these sensory disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.
See full target information Pcdh15
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell research 35:243-264 PubMed39966628

2025

A force-sensitive adhesion GPCR is required for equilibrioception.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Yang,Shu-Hua Zhou,Qi-Yue Zhang,Zhi-Chen Song,Wen-Wen Liu,Yu Sun,Ming-Wei Wang,Xiao-Long Fu,Kong-Kai Zhu,Ying Guan,Jie-Yu Qi,Xiao-Hui Wang,Yu-Nan Sun,Yan Lu,Yu-Qi Ping,Yue-Tong Xi,Zhen-Xiao Teng,Lei Xu,Peng Xiao,Zhi-Gang Xu,Wei Xiong,Wei Qin,Wei Yang,Fan Yi,Ren-Jie Chai,Xiao Yu,Jin-Peng Sun

Open medicine (Warsaw, Poland) 19:20241070 PubMed39588388

2024

Significant role and the underly mechanism of cullin-1 in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Hao,Fei Lin,Weili Kong,Hanbing Shi,Haiying Dong,Zhanjiang Guan,Guohua Liu,Xiao Wang,Li Wang,Moran Liu,Yunfei Jiang
View all publications
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Product promise

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