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Rabbit Recombinant Monoclonal PCDH15 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (AB251375), expandable thumbnail
  • Western blot - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (AB251375), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.

Alternative names

Protocadherin-15, Pcdh15

Recommended products

Rabbit Recombinant Monoclonal PCDH15 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19600
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251375 is the carrier-free version of Anti-PCDH15 antibody [EPR19600] ab202560.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PCDH15 also known as Protocadherin-15 is a protein encoded by the PCDH15 gene. Mechanically this protein functions as an adhesion molecule in the body playing an important role in cell-cell interactions. PCDH15 has a molecular mass of approximately 223 kDa. It has a widespread expression but is particularly found in sensory cells in the inner ear and in the retina of the eye highlighting its importance in sensory functions.

Biological function summary

PCDH15 participates in the formation of tip-link structures within the inner ear. This protein is a component of the mechanotransduction complex which translates mechanical stimuli into electrical signals. PCDH15 works alongside other proteins like Cadherin 23 another protocadherin to maintain the integrity of hair cells. Its role is essential for proper auditory and visual signal processing contributing to the sense of hearing and vision.

Pathways

PCDH15 interacts with the mechanotransduction pathway in sensory cells. This pathway plays a role in converting mechanical stimuli such as sound waves in the cochlea into nerve impulses. Cadherin 23 is closely related in this pathway and together these proteins maintain the hair cell structure necessary for mechanotransduction. The interplay between these proteins ensures the transduction of sensory information impacting cellular responses in sensory organs.

Associated diseases and disorders

PCDH15 mutations associate with Usher syndrome type 1F and non-syndromic hearing loss. Usher syndrome is a genetic condition involving hearing loss and vision impairment. PCDH15 mutations disrupt the mechanotransduction complex affecting sensory perception. Cadherin 23 also connects to these disorders as mutations in this protein can similarly lead to issues in hearing and vision. Understanding PCDH15 and its interactors is critical in addressing these sensory disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)

    This data was developed using Anti-PCDH15 antibody [EPR19600] ab202560, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged mouse PCDH15 expression construct or GFP vector only, labeling PCDH15 with Anti-PCDH15 antibody [EPR19600] ab202560 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution (red). Confocal image showing positive staining on HEK-293 cells transfected with a GFP-tagged mouse PCDH15 expression construct. This mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 (Alexa Fluor ® 647 Goat anti-Mouse secondary) at 1/1000 dilution (white). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution.

  • Western blot - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375), expandable thumbnail

    Western blot - Anti-PCDH15 antibody [EPR19600] - BSA and Azide free (ab251375)

    This data was developed using Anti-PCDH15 antibody [EPR19600] ab202560, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16481439).

    The mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

    WB detection of endogenous PCDH15 is not shown due to unavailability of appropriate testing material.

    All lanes: Western blot - Anti-PCDH15 antibody [EPR19600] (Anti-PCDH15 antibody [EPR19600] ab202560) at 1/1000 dilution

    Lane 1: 293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with an empty expression vector at 10 µg

    Lane 2: 293T whole cell lysate transfected with a DDDK-tagged mouse PCDH15 construct at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 216 kDa

    Observed band size: 260 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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