AB139274

Anti-PCDH7 antibody [OTI2G6]

Name: Anti-PCDH7 antibody [OTI2G6] Mouse monoclonal (ab139274) | Abcam
Brand: Abcam Limited
SKU: AB139274
Price: 470 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Mouse Monoclonal PCDH7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Dog, African green monkey, Recombinant fragment - Human, Human, Rat samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PCDH7.

View Alternative Names

BHPCDH, PCDH7, Protocadherin-7, Brain-heart protocadherin, BH-Pcdh

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (AB139274)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (AB139274)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labelling PCDH7 with ab139274 at 1/150 dilution.

Unknown

Western blot - Anti-PCDH7 antibody [OTI2G6] (AB139274)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206769)

All lanes:

Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7 at 5 µg

Predicted band size: 116 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (AB139274)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (AB139274)

Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY PCDH7 using ab139274 at 1/100 dilution.

Unknown

Western blot - Anti-PCDH7 antibody [OTI2G6] (AB139274)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206740)

All lanes:

Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution

Lane 1:

HepG2 cell extract at 35 µg

Lane 2:

HeLa cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 cell extract at 35 µg

Lane 5:

COS7 cell extract at 35 µg

Lane 6:

Jurkat cell extract at 35 µg

Lane 7:

MDCK cell extract at 35 µg

Lane 8:

PC12 cell extract at 35 µg

Lane 9:

MCF7 cell extract at 35 µg

Predicted band size: 116 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2G6

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PCDH7.

O60245

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from 2G6 to OTI2G6, both clone numbers name the same clone.

Dilute in PBS (pH7.3) before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCDH7 also known as Protocadherin 7 is a member of the protocadherin family falling under the broader cadherin superfamily. It has a mass of approximately 120 kDa. PCDH7 is a type I transmembrane protein known for its role in cellular adhesion. It is expressed in several tissues with higher expression in the nervous system and lungs. Its expression pattern suggests a role in specific tissue differentiation and development. As a protocadherin PCDH7 facilitates cell-cell interactions essential for maintaining tissue architecture.

Biological function summary

PCDH7 contributes to cell signaling and adhesion processes. It is not currently known to be a part of a large recognized complex but its interactions at the cell membrane influence cellular communication networks. In the nervous system PCDH7 supports neuronal connectivity and synaptic function aiding in the establishment of neural circuits. It also plays a part in epithelial cell layer formation aiding in maintaining proper cell alignment and barrier function. This supports PCDH7's classification as an important player in structural tissue integrity and intercellular communication.

Pathways

PCDH7 is involved in key signaling pathways that govern cellular adhesion and signaling dynamics. It plays a significant role in the Wnt signaling pathway important for cell proliferation differentiation and migration. PCDH7 interacts with proteins such as β-catenin which is a pivotal part of the Wnt pathway. Its regulation of cellular interactions helps maintain cellular structural organization ensuring normal development and response to cellular signals in specific tissues.

Researchers have linked PCDH7 to respiratory disorders and certain cancers. Studies demonstrate increased expression of PCDH7 in lung adenocarcinoma indicating its potential role in tumorigenesis. It associates with proteins like E-cadherin where misregulation can lead to altered cell adhesion and metastasis. PCDH7's involvement in this dysregulation hints at its potential as a biomarker for diagnosis or a target for therapeutic interventions in cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PCDH7

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106273 PubMed36936789

2023

Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xiao,Min Hu,Qiyan Lin,Ting Zhang,Siying Li,Linjuan Shu,Xiuli Song,Xiaoyong Xu,Wentong Meng,Xue Li,Hong Xu,Xianming Mo

International journal of biological sciences 18:1491-1507 PubMed35280687

2022

Circular RNA circDVL1 inhibits clear cell renal cell carcinoma progression through the miR-412-3p/PCDH7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yunjing Zhang,Xinwan Su,Qiongzi Qiu,Yuan Yuan,Chunhua Weng,Sailan Zou,Yan Tian,Weidong Han,Pengyuan Liu,Xingyi Guo,Jianhua Mao,Xianghui Fu,Ping Wang,Weiqiang Lin

Nature protocols 16:2542-2563 PubMed33864055

2021

Directed differentiation of human induced pluripotent stem cells to hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Vallverdú,Raquel A Martínez García de la Torre,Inge Mannaerts,Stefaan Verhulst,Ayla Smout,Mar Coll,Silvia Ariño,Teresa Rubio-Tomás,Beatriz Aguilar-Bravo,Celia Martínez-Sánchez,Delia Blaya,Catherine M Verfaillie,Leo A van Grunsven,Pau Sancho-Bru

Scientific reports 10:10951 PubMed32616769

2020

PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Meghan Kerrisk Campbell,Irene Tom,Oded Foreman,Jesse E Hanson,Morgan Sheng

Frontiers in cell and developmental biology 8:329 PubMed32457908

2020

PCDH7 Inhibits the Formation of Homotypic Cell-in-Cell Structure.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Wang,Ang Chen,Banzhan Ruan,Zubiao Niu,Yan Su,Hongquan Qin,You Zheng,Bo Zhang,Lihua Gao,Zhaolie Chen,Hongyan Huang,Xiaoning Wang,Qiang Sun

Bioscience reports 40: PubMed32285917

2020

MiR-139-5p influences hepatocellular carcinoma cell invasion and proliferation capacities via decreasing SLITRK4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jisiguleng Wu,Tong Zhang,Yubo Chen,Sigaowa Ha

Molecular therapy. Nucleic acids 20:545-557 PubMed32330872

2020

Recurrence-Associated Long Non-coding RNA LNAPPCC Facilitates Colon Cancer Progression via Forming a Positive Feedback Loop with PCDH7.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Li,Zhiqiang Li,Hongxing Wan,Xifeng Tang,Han Wang,Fang Chai,Meng Zhang,Baochun Wang

Molecular cancer research : MCR 17:594-603 PubMed30409919

2018

Modulation of Mutant -Driven Lung Tumorigenesis by Gain or Loss of PCDH7 Function.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorong Zhou,Mahesh S Padanad,Bret M Evers,Bethany Smith,Nicole Novaresi,Shruthy Suresh,James A Richardson,Emily Stein,Jingfei Zhu,Robert E Hammer,Kathryn A O'Donnell

Cell stem cell 23:101-113.e7 PubMed30049452

2018

Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mar Coll,Luis Perea,Ruben Boon,Sofia B Leite,Julia Vallverdú,Inge Mannaerts,Ayla Smout,Adil El Taghdouini,Delia Blaya,Daniel Rodrigo-Torres,Isabel Graupera,Beatriz Aguilar-Bravo,Christophe Chesne,Mustapha Najimi,Etienne Sokal,Juan José Lozano,Leo A van Grunsven,Catherine M Verfaillie,Pau Sancho-Bru

Experimental cell research 366:71-80 PubMed29548751

2018

Overexpression of protocadherin 7 inhibits neuronal survival by downregulating BIRC5 in vitro.

Applications

Species

Mouse

Huajuan Xiao,Ziling Sun,Jun Wan,Shengtao Hou,Yi Xiong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCDH7 antibody [OTI2G6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (AB139274)

Western blot - Anti-PCDH7 antibody [OTI2G6] (AB139274)

Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (AB139274)

Western blot - Anti-PCDH7 antibody [OTI2G6] (AB139274)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise