Rabbit Polyclonal PCDHA1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PCDHA1 aa 1-250.
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Publications
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- Clinical breast cancer 23:397-4072023PCDHA1 High Expression is Associated With Poor Prognosis and Correlated With Immune Cell Infiltration in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawei Du,Kaidi WuPubMed 36858841
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PCDHA1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5I3
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.2-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Target data
Function
Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Alternative names
Protocadherin alpha-1, PCDH-alpha-1, PCDHA1
Recommended products
Rabbit Polyclonal PCDHA1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PCDHA1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PCDHA1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5I3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCDHA1 also known as Protocadherin alpha-1 is a member of the protocadherin family which belong to the cadherin superfamily. This protein has a mass of approximately 105 kDa. It plays a role in forming calcium-dependent cell adhesion by binding homophilically which is important for maintaining the strength and positional accuracy of cell-cell contacts. PCDHA1 is primarily expressed in the brain particularly in the neural tissue where it contributes to the diversity and specificity of synaptic connections.
- Biological function summary
PCDHA1 plays a role in the development and maintenance of neuronal networks. This protein seems to be part of a complex involved in synaptic formation and modulation. Researchers believe PCDHA1 contributes to the establishment of specific neuronal cell assemblies and supports the dynamic patterning of neuronal connectivity. The protocadherin family including PCDHA1 actively regulates the diversity of synapses required for proper neural circuit formation.
- Pathways
PCDHA1 participates in the cell adhesion pathway and neural differentiation pathway. In these pathways PCDHA1 interacts with other protocadherins and likely cooperates with classical cadherins to mediate precise cellular signaling events. The target closely associates with proteins like PCDHA4 which also influences neural circuit development indicating overlapping roles across protocadherin family members in maintaining neural structure and function.
- Associated diseases and disorders
PCDHA1 has connections to neurological conditions such as autism spectrum disorders and epilepsy. Alterations in PCDHA1 expression or mutations can disrupt synaptic connectivity leading to these conditions. The protein's involvement with neurological diseases also points to its relationship with N-cadherin involved in similar pathways suggesting that these networks' proper function is essential to prevent these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDHA1 antibody (ab231152)
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PCDHA1 with ab231152 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-PCDHA1 antibody (ab231152)
All lanes: Western blot - Anti-PCDHA1 antibody (ab231152) at 1 µg/mL
All lanes: Human serum
Predicted band size: 103 kDa, 156 kDa, 42 kDa, 68 kDa
Western blot - Anti-PCDHA1 antibody (ab231152)
All lanes: Western blot - Anti-PCDHA1 antibody (ab231152) at 2 µg/mL
All lanes: Recombinant human PCDHA1 protein
Predicted band size: 103 kDa
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