Anti-PCDHAC2 antibody [EPR28522-31] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-PCDHAC2 antibody [EPR28522-31] - BSA and Azide free (AB315032)

This data was developed using ab315031, the same antibody clone in a different buffer formulation.

PCDHAC2 was immunoprecipitated from 0.35 mg Mouse hippocampus tissue lysate with ab315031 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315031 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : ab315031 IP in Mouse hippocampus tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315031 in mouse hippocampus tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PCDHAC2 antibody [EPR28522-31] (<a href='/en-us/products/primary-antibodies/pcdhac2-antibody-epr28522-31-ab315031'>ab315031</a>) at 1/30 dilution

All lanes:

Mouse hippocampus tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• WB

Supplier Data

Western blot - Anti-PCDHAC2 antibody [EPR28522-31] - BSA and Azide free (AB315032)

This data was developed using ab315031, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST,

Low expression : heart, liver.

The samples are unboiled.

The identity of the lower MW bands at approximately 40kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure Time : Lane1-4 : 48 Seconds; Lane 5 : 10 Seconds.

All lanes:

Western blot - Anti-PCDHAC2 antibody [EPR28522-31] (<a href='/en-us/products/primary-antibodies/pcdhac2-antibody-epr28522-31-ab315031'>ab315031</a>) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

Human heart tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Lane 5:

Human spinal cord tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 130 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-PCDHAC2 antibody [EPR28522-31] - BSA and Azide free (AB315032)

This data was developed using ab315031, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart.

The samples are unboiled.

The identity of the lower MW bands at approximately 40kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure Time : Lane 1-3 : 48 Seconds; Lane 4- 5 : 26 Seconds

All lanes:

Western blot - Anti-PCDHAC2 antibody [EPR28522-31] (<a href='/en-us/products/primary-antibodies/pcdhac2-antibody-epr28522-31-ab315031'>ab315031</a>) at 1/1000 dilution

Lane 1:

Mouse hypothalamus tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Rat hippocampus tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-PCDHAC2 antibody [EPR28522-31] - BSA and Azide free (AB315032)

This data was developed using ab315031, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST,

Low expression : liver, testis.

The samples are unboiled.

The identity of the lower MW bands at approximately 40kDa is unknown.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PCDHAC2 antibody [EPR28522-31] (<a href='/en-us/products/primary-antibodies/pcdhac2-antibody-epr28522-31-ab315031'>ab315031</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Lane 6:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa,124 kDa

false

Exposure time: 15s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-PCDHAC2 antibody [EPR28522-31] - BSA and Azide free (AB315032)

This data was developed using ab315031, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab315031 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human PCDHAC2.

Antigen concentration : 1000 ng/ml