AB134391

Anti-PCF11 antibody

Name: Anti-PCF11 antibody. Rabbit polyclonal (ab134391) | Abcam
Brand: Abcam Limited
SKU: AB134391

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(3 Publications)

Rabbit Polyclonal PCF11 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PCF11 aa 1500 to C-terminus.

View Alternative Names

KIAA0824, PCF11, Pre-mRNA cleavage complex 2 protein Pcf11, Pre-mRNA cleavage complex II protein Pcf11

2 Images

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Immunoprecipitation - Anti-PCF11 antibody (AB134391)

Detection of PCF11 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab134391 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-PCF11 antibody (ab134391)

Predicted band size: 173 kDa

false

Unknown

Western blot - Anti-PCF11 antibody (AB134391)

All lanes:

Western blot - Anti-PCF11 antibody (ab134391) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 173 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PCF11 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94913

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PCF11 occasionally referred to as "Polypeptide Cleavage Factor 1" is an essential player in the transcription termination process. This protein serves as a component of the cleavage and polyadenylation specificity factor complex enabling the final stages of pre-mRNA synthesis. PCF11 has a molecular mass of approximately 63 kDa and is highly expressed in the nucleus where transcription occurs. It is found in many types of tissues indicating its wide-ranging roles in cellular biology.

Biological function summary

PCF11 influences the even regulation of gene expression by aiding in the removal of RNA polymerase II from DNA which helps in the proper conclusion of transcription. It works as part of a larger complex partnering with other factors to ensure that mRNA is properly released and processed which is important for functional mRNA maturation. These actions highlight its importance in RNA metabolism and stability.

Pathways

PCF11 is intricately involved in the transcriptional regulation pathway especially influencing the maturation of mRNA through interactions with RNA polymerase II. It closely coordinates its activities with proteins such as CTD phosphatase and SCAF8 directing the proper progression and termination of transcription. Additionally its involvement in RNA processing links it to the spliceosome-mediated mRNA decay pathway ensuring accurate gene expression control.

Mutations and disruptions in PCF11 expression are linked to neurological disorders and various carcinomas. PCF11 anomalies can lead to defective RNA metabolism affecting neural development and cancer proliferation. In cancer it has connections with overexpressed proteins such as MYC which promotes malignant growth through misregulated transcription termination similarly impacting neurological health when dysregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of pre-mRNA cleavage complex II, which promotes transcription termination by RNA polymerase II.

See full target information PCF11

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:8165-8183 PubMed38842922

2024

Increased transcriptional elongation and RNA stability of GPCR ligand binding genes unveiled via RNA polymerase II degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Bao,Junyi Zhu,Tingxin Shi,Yongpeng Jiang,Boyuan Li,Jie Huang,Xiong Ji

Molecular cell 74:158-172.e9 PubMed30819644

2019

Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination.

Applications

Unspecified application

Species

Unspecified reactive species

Kinga Kamieniarz-Gdula,Michal R Gdula,Karin Panser,Takayuki Nojima,Joan Monks,Jacek R Wiśniewski,Joey Riepsaame,Neil Brockdorff,Andrea Pauli,Nick J Proudfoot

Genes & development 31:2175-2185 PubMed29196535

2017

WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Volanakis,Kinga Kamieniarz-Gdula,Margarita Schlackow,Nick J Proudfoot

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCF11 antibody

Immunoprecipitation - Anti-PCF11 antibody (AB134391)

Western blot - Anti-PCF11 antibody (AB134391)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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