AB278502

Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PCGF1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

NSPC1, RNF68, PCGF1, Polycomb group RING finger protein 1, Nervous system Polycomb-1, RING finger protein 68, NSPc1

5 Images

Flow Cytometry (Intracellular) - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

This data was developed using ab259943, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling PCGF1 with ab259943 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

This data was developed using ab259943, the same antibody clone in a different buffer formulation.

PCGF1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab259943 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2 : ab259943 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259943 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] (<a href='/en-us/products/primary-antibodies/pcgf1-antibody-epr23757-38-ab259943'>ab259943</a>)

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

This data was developed using ab259943, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling PCGF1 with ab259943 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

This data was developed using ab259943, the same antibody clone in a different buffer formulation.

PCGF1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab259943 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lane 2 : ab259943 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259943 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] (<a href='/en-us/products/primary-antibodies/pcgf1-antibody-epr23757-38-ab259943'>ab259943</a>)

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Lab

Western blot - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

This data was developed using ab259943, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 125 seconds

All lanes:

Western blot - Anti-PCGF1 antibody [EPR23757-38] (<a href='/en-us/products/primary-antibodies/pcgf1-antibody-epr23757-38-ab259943'>ab259943</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 7:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Unconjugated

Anti-PCGF1 antibody [EPR23757-38]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23757-38

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab278502 is the carrier-free version of ab259943.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCGF1 also known as Polycomb group RING finger protein 1 is a component of the Polycomb Group (PcG) proteins. It has a mass of approximately 36 kDa. This protein functions mechanically as part of the Polycomb Repressive Complex 1 (PRC1) where it plays a role in chromatin modification and gene silencing. PCGF1 is expressed in various tissues including embryonic stem cells and can affect cellular differentiation and proliferation through epigenetic regulation.

Biological function summary

PCGF1 serves as a regulatory factor within the Polycomb Repressive Complex 1 which is involved in maintaining the transcriptionally repressive state of many genes through histone modifications. PCGF1 assists in the ubiquitination of histone H2A an essential modification for chromatin conformation that leads to transcriptional repression. Through this modification PCGF1 contributes to the control of gene networks necessary for development and cellular identity.

Pathways

PCGF1 is a part of the Polycomb Repressive Complex 1 pathway and contributes to the intricate network of gene expression regulation. The complex participates in the Wnt signaling pathway which plays a critical role in embryonic development and cell growth. Through its participation in these pathways PCGF1 interacts with other proteins like BMI1 and RING1 which help in establishing transcriptional repression important for proper cellular function.

Alterations in PCGF1 have been linked to certain cancers and neurological disorders. Dysregulation of PCGF1 expression or function can lead to aberrant cell proliferation contributing to oncogenesis in cancers such as leukemia. Moreover defects in the PcG pathway involving PCGF1 are associated with neurodevelopmental disorders where altered expression patterns of target genes disrupt normal neural function. These conditions highlight the connections between PCGF1 and proteins like EED and SUZ12 which also play roles in the regulation of gene expression and are implicated in similar disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down-regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 26151332). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed : 26151332). Regulates the expression of DPPA4 and NANOG in the NT2 embryonic carcinoma cells (PubMed : 26687479).

See full target information PCGF1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

Flow Cytometry (Intracellular) - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

Western blot - Anti-PCGF1 antibody [EPR23757-38] - BSA and Azide free (AB278502)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise