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AB254965

Anti-PCGF3 antibody

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(1 Publication)

Rabbit Polyclonal PCGF3 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PCGF3 aa 100-200.

View Alternative Names

RNF3, RNF3A, PCGF3, Polycomb group RING finger protein 3, RING finger protein 3A

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCGF3 antibody (AB254965)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PCGF3 antibody (AB254965)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling PCGF3 using ab254965 at 4µg/ml (green) in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PCGF3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q3KNV8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCGF3 also known as Polycomb Group RING Finger 3 is a protein that plays a mechanical role as part of the Polycomb group (PcG) proteins. The molecular mass of PCGF3 typically ranges around 35 kDa. It is expressed in various tissues with a notable presence in embryonic stem cells and during early developmental stages. PCGF3 functions as a component of Polycomb Repressive Complex 1 (PRC1) and influences chromatin modification impacting gene expression.
Biological function summary

PCGF3 influences gene silencing and epigenetic regulation. This protein forms part of the PRC1 complex which is important for maintaining the transcriptionally repressive state of many genes throughout development. PCGF3 alongside other PcG proteins helps to regulate chromatin structure by mediating the mono-ubiquitination of histone H2A one of its key functions in chromatin modification.

Pathways

The PCGF3 protein takes part in the Polycomb pathway which impacts the transcriptional silencing of Hox genes critical for body patterning and development. It relates closely to other proteins within this pathway such as BMI1 which is also a member of the PRC1 complex. Additionally PCGF3 plays a role in the regulation of the WNT signaling pathway influencing cellular differentiation and proliferation.

PCGF3 has links to various cancers and developmental disorders given its role in gene silencing and epigenetic regulation. Changes in its expression can lead to aberrant activation or repression of target genes potentially contributing to tumorigenesis. Research indicates that alterations in WNT signaling involving PCGF3 and related proteins such as RING1B could play a role in the pathogenesis of colorectal cancer and other malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed : 26151332). Plays a redundant role with PCGF5 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity).
See full target information PCGF3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:9424-9443 PubMed34365507

2021

SDE2 is an essential gene required for ribosome biogenesis and the regulation of alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Jess Floro,Anqi Dai,Abigail Metzger,Alexandra Mora-Martin,Neil J Ganem,Daniel Cifuentes,Ching-Shyi Wu,Jasbir Dalal,Shawn M Lyons,Adam Labadorf,Rachel L Flynn
View all publications
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