Anti-PCGF5 antibody [EP19476]

• WB

Supplier Data

Western blot - Anti-PCGF5 antibody [EP19476] (AB201511)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 30 seconds; Lane 5-7 : 3 minutes.

All lanes:

Western blot - Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 5:

Rat testis lysate at 10 µg

Lane 6:

Human testis lysate at 10 µg

Lane 7:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-PCGF5 antibody [EP19476] (AB201511)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Mouse ovary lysate at 10 µg

Lane 3:

Mouse thymus lysate at 10 µg

Lane 4:

mES-D3 (Mouse embryo stem cell line) whole cell lysate at 10 µg

Lane 5:

F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Exposure time: 3min

• WB

CiteAb

Western blot - Anti-PCGF5 antibody [EP19476] (AB201511)

PCGF5 western blot using anti-PCGF5 antibody [EP19476] ab201511. Publication image and figure legend from Yan, Y., Zhao, W., et al., 2017, Sci Rep, PubMed 28393894.

ab201511 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab201511 please see the product overview.

Pcgf1 is dispensable for ES cell self-renewal.(A) Representative phase images of WT and Pcgf1−/− ES cell colonies. WT and Pcgf1−/− ES cell colonies were photographed at days 5 and 7 after seeding single-cell suspensions onto MEF-feeder layers. Bar graph showed the mean diameter of 20 random ES cell colonies from three independent experiments. Data are presented as mean ± standard deviation of triplicate experiments. Images were taken at 100× magnification at days 5 and 7. (B) Cell cycle distribution of WT and Pcgf1−/− ES cell was analysed by flow cytometry. Top right corner data represented the percentage of cells within the different cell cycle phases. (C) The alkaline phosphatase (AP) activity was examined and ES cell colonies were photographed under microscope. Images were taken at 100× magnification at days 7. (D) Protein levels were determined by Western blot in WT and Pcgf1−/− ES cells (BcoR, Ring1B and Rybp belong to PRC1.1, Nanog, Oct4, Sox2 are pluripotency markers, Pcgf5 is a component of PRC1.5 and Suz12 is the core subunit of PRC2, Actin was shown as a loading control).

false