Skip to main content

Rabbit Recombinant Monoclonal PCGF6 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 3 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-PCGF6 antibody [EPR19450] (AB200038), expandable thumbnail
  • Western blot - Anti-PCGF6 antibody [EPR19450] (AB200038), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (AB200038), expandable thumbnail
  • Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (AB200038), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

ICC is recommended for mouse only.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.

Alternative names

Mblr, Rnf134, Pcgf6, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134

Recommended products

Rabbit Recombinant Monoclonal PCGF6 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19450
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PCGF6 also known as Polycomb group RING finger protein 6 plays a role in regulating gene expression. This protein with a molecular mass of about 36 kDa is part of the Polycomb Group (PcG) proteins that epigenetically silence genes preventing their expression. Scientists have observed PCGF6 expression in embryonic stem cells as well as in various tissues hinting at its broad potential impact on cellular processes. The protein contains a RING finger motif which suggests it functions as an E3 ubiquitin ligase tagging other proteins for modification or degradation.

Biological function summary

PCGF6 participates in transcriptional repression through its involvement in the non-canonical Polycomb Repressive Complex 1 (PRC1). The protein acts by aiding the assembly of PRC1 alongside other constituents such as RING1B to modify histones and maintain stem cell characteristics. This activity is important for cell differentiation and maintaining the balance between pluripotency and lineage specification during development. Disruptions in PCGF6 function may influence epigenetic landscapes significantly thereby impacting cellular identity and behavior.

Pathways

PCGF6 integrates its activity with key biological systems such as the Wnt signaling and the polycomb repression pathways. Within these pathways PCGF6 interacts with core PRC1 components like RING1B to affect gene silencing and chromatin remodeling. These interactions allow the protein to serve as a critical node in controlling genetic hierarchies that regulate embryogenesis and other developmental processes. The pathway network involving PCGF6 and PRC1 provides essential insights into the fine-tuning of gene expressions needed for cellular differentiation.

Associated diseases and disorders

Aberrations in PCGF6 expression or function have connections to cancer and developmental disorders. Altered PCGF6 activity can lead to dysregulated expression of genes that are normally repressed fostering oncogenic or detrimental developmental outcomes. In the context of cancer its interaction with components such as BMI1 helps to drive tumor progression by supporting unchecked cell proliferation. Furthermore understanding these interactions highlights potential therapeutic avenues by targeting the components influenced by PCGF6 offering opportunities for better management of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038), expandable thumbnail

    Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 3 minutes; Lane 5: 10 seconds.

    All lanes: Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

    Lane 1: Mouse testis lysate at 10 µg

    Lane 2: Mouse thymus lysate at 10 µg

    Lane 3: Mouse ovary lysate at 10 µg

    Lane 4: Mouse embryo lysate at 10 µg

    Lane 5: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa

    Observed band size: 42 kDa

  • Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038), expandable thumbnail

    Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2 : 3 minutes; Lane 3/4: 30 seconds.

    All lanes: Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

    Lane 1: Rat testis lysate at 10 µg

    Lane 2: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 3: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 4: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa

    Observed band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (ab200038), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (ab200038)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling PCGF6 with ab200038 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab200038 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (ab200038), expandable thumbnail

    Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (ab200038)

    ab200038 Immunoprecipitating PCGF6 in F9(mouse embryonal carcinoma) whole cell lysate. 350μg of cell lysate was incubated with primary antibody (2 μg/ml). For western blotting ab200038 (1/1000) was used to confirm successful immunoprecipation. For detection VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000.

    Lane 1: (Input) F9(mouse embryonal carcinoma) whole cell lysate 10μg
    Lane 2: (+) F9 whole cell lysate
    Lane 3: (-) Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200038 in F9 whole cell lysate

    All lanes: Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (ab200038)

    Predicted band size: 39 kDa

    Observed band size: 42 kDa

    Exposure time: 8s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com