AB200038

Anti-PCGF6 antibody [EPR19450]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal PCGF6 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Mblr, Rnf134, Pcgf6, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (AB200038)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (AB200038)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling PCGF6 with ab200038 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cells. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab200038 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.

-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (AB200038)

ab200038 Immunoprecipitating PCGF6 in F9(mouse embryonal carcinoma) whole cell lysate. 350μg of cell lysate was incubated with primary antibody (2 μg/ml). For western blotting ab200038 (1/1000) was used to confirm successful immunoprecipation. For detection VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000.

Lane 1 : (Input) F9(mouse embryonal carcinoma) whole cell lysate 10μg
Lane 2 : (+) F9 whole cell lysate
Lane 3 : (-) Rabbit monoclonal IgG (ab172730) instead of ab200038 in F9 whole cell lysate

All lanes:

Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (ab200038)

Predicted band size: 39 kDa

Observed band size: 42 kDa

false

Exposure time: 8s

Supplier Data

Western blot - Anti-PCGF6 antibody [EPR19450] (AB200038)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 3 minutes; Lane 5 : 10 seconds.

All lanes:

Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Mouse thymus lysate at 10 µg

Lane 3:

Mouse ovary lysate at 10 µg

Lane 4:

Mouse embryo lysate at 10 µg

Lane 5:

F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 42 kDa

false

Supplier Data

Western blot - Anti-PCGF6 antibody [EPR19450] (AB200038)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 3 minutes; Lane 3/4 : 30 seconds.

All lanes:

Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

Lane 1:

Rat testis lysate at 10 µg

Lane 2:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 3:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 42 kDa

false

Carrier free

Anti-PCGF6 antibody [EPR19450] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19450

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "ICC is recommended for mouse only." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCGF6 also known as Polycomb group RING finger protein 6 plays a role in regulating gene expression. This protein with a molecular mass of about 36 kDa is part of the Polycomb Group (PcG) proteins that epigenetically silence genes preventing their expression. Scientists have observed PCGF6 expression in embryonic stem cells as well as in various tissues hinting at its broad potential impact on cellular processes. The protein contains a RING finger motif which suggests it functions as an E3 ubiquitin ligase tagging other proteins for modification or degradation.

Biological function summary

PCGF6 participates in transcriptional repression through its involvement in the non-canonical Polycomb Repressive Complex 1 (PRC1). The protein acts by aiding the assembly of PRC1 alongside other constituents such as RING1B to modify histones and maintain stem cell characteristics. This activity is important for cell differentiation and maintaining the balance between pluripotency and lineage specification during development. Disruptions in PCGF6 function may influence epigenetic landscapes significantly thereby impacting cellular identity and behavior.

Pathways

PCGF6 integrates its activity with key biological systems such as the Wnt signaling and the polycomb repression pathways. Within these pathways PCGF6 interacts with core PRC1 components like RING1B to affect gene silencing and chromatin remodeling. These interactions allow the protein to serve as a critical node in controlling genetic hierarchies that regulate embryogenesis and other developmental processes. The pathway network involving PCGF6 and PRC1 provides essential insights into the fine-tuning of gene expressions needed for cellular differentiation.

Aberrations in PCGF6 expression or function have connections to cancer and developmental disorders. Altered PCGF6 activity can lead to dysregulated expression of genes that are normally repressed fostering oncogenic or detrimental developmental outcomes. In the context of cancer its interaction with components such as BMI1 helps to drive tumor progression by supporting unchecked cell proliferation. Furthermore understanding these interactions highlights potential therapeutic avenues by targeting the components influenced by PCGF6 offering opportunities for better management of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.

See full target information Pcgf6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113059 PubMed40727931

2025

MGA directly recruits SETDB1/ATF7IP for histone H3K9me3 mark on meiosis-related genes in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kousuke Uranishi,Masataka Hirasaki,Masazumi Nishimoto,Robert J Klose,Akihiko Okuda,Ayumu Suzuki

Nucleic acids research 51:3130-3149 PubMed36772830

2023

Systematic identification of factors involved in the silencing of germline genes in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hala Al Adhami,Judith Vallet,Celia Schaal,Paul Schumacher,Anaïs Flore Bardet,Michael Dumas,Johana Chicher,Philippe Hammann,Sylvain Daujat,Michael Weber

Science advances 6:eaax5692 PubMed32270030

2020

Parallel PRC2/cPRC1 and vPRC1 pathways silence lineage-specific genes and maintain self-renewal in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

J A Zepeda-Martinez,C Pribitzer,J Wang,D Bsteh,S Golumbeanu,Q Zhao,T R Burkard,B Reichholf,S K Rhie,J Jude,H F Moussa,J Zuber,O Bell

Protein & cell 10:709-725 PubMed31041782

2019

PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaona Huang,Chao Wei,Fenjie Li,Lumeng Jia,Pengguihang Zeng,Jiahe Li,Jin Tan,Tuanfeng Sun,Shaoshuai Jiang,Jia Wang,Xiuxiao Tang,Qingquan Zhao,Bin Liu,Limin Rong,Cheng Li,Junjun Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCGF6 antibody [EPR19450]

Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (AB200038)

Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (AB200038)

Western blot - Anti-PCGF6 antibody [EPR19450] (AB200038)

Western blot - Anti-PCGF6 antibody [EPR19450] (AB200038)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise