Anti-PCGF6 antibody [EPR19450] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (AB251294)

This data was developed using ab200038, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling PCGF6 with ab200038 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cells. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab200038 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.

-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (AB251294)

This data was developed using ab200038&44; the same antibody clone in a different buffer formulation.

ab200038 Immunoprecipitating PCGF6 in F9 (mouse embryonal carcinoma) whole cell lysate. 350μg of cell lysate was incubated with primary antibody (2 μg/ml). For western blotting, ab200038 (1/1000) was used to confirm successful immunoprecipation. For detection VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000.

Lane 1 : (Input) F9 (mouse embryonal carcinoma) whole cell lysate 10μg

Lane 2 : (+) F9 whole cell lysate

Lane 3 : (-) Rabbit monoclonal IgG (ab172730) instead of ab200038 in F9 whole cell lysate

All lanes:

Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (<a href='/en-us/products/primary-antibodies/pcgf6-antibody-epr19450-ab200038'>ab200038</a>)

Predicted band size: 39 kDa

Observed band size: 42 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (AB251294)

This data was developed using ab200038, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1-4 : 3 minutes; Lane 5 : 10 seconds.

All lanes:

Western blot - Anti-PCGF6 antibody [EPR19450] (<a href='/en-us/products/primary-antibodies/pcgf6-antibody-epr19450-ab200038'>ab200038</a>) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Mouse thymus lysate at 10 µg

Lane 3:

Mouse ovary lysate at 10 µg

Lane 4:

Mouse embryo lysate at 10 µg

Lane 5:

F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 42 kDa

false

• WB

Supplier Data

Western blot - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (AB251294)

This data was developed using ab200038, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 3 minutes; Lanes 3-4 : 30 seconds.

All lanes:

Western blot - Anti-PCGF6 antibody [EPR19450] (<a href='/en-us/products/primary-antibodies/pcgf6-antibody-epr19450-ab200038'>ab200038</a>) at 1/1000 dilution

Lane 1:

Rat testis lysate at 10 µg

Lane 2:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 3:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 42 kDa

false