Rabbit Polyclonal PCID1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EIF3M.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human EIF3M. Database link Q7L2H7
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17403899, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17403899). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). (Microbial infection) May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).
Alternative names
HFLB5, PCID1, GA17, PNAS-125, EIF3M, Eukaryotic translation initiation factor 3 subunit M, eIF3m, Fetal lung protein B5, PCI domain-containing protein 1, hFL-B5
Recommended products
Rabbit Polyclonal PCID1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EIF3M.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human EIF3M. Database link Q7L2H7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCID1 also known as PCI domain containing 1 is a protein with a molecular weight of approximately 125 kDa. It plays an essential role in cellular processes as it is a component of the nuclear structure influencing key regulatory functions. PCID1 is broadly expressed with higher levels detected in tissues such as the brain heart and liver. Its interaction with cellular machinery highlights its significance in maintaining cellular homeostasis.
- Biological function summary
PCID1 operates as part of the central mRNA export machinery within the Transcription Export (TREX) complex. This complex is important for the proper export of mRNA from the nucleus to the cytoplasm thereby influencing gene expression. PCID1 directly facilitates the binding and release of mRNA molecules ensuring their correct processing and transport. Disruption in its function can impede mRNA export affecting cellular function and gene expression.
- Pathways
PCID1 is integrally involved in the mRNA transport pathway and the gene expression pathway. Within these pathways it interacts closely with proteins like Aly/REF export factor (ALYREF) and nuclear RNA export factor 1 (NXF1). These proteins work in concert with PCID1 to mediate the efficient export of mature mRNA transcripts impacting various biological processes such as cellular metabolism growth and response to stimuli.
- Associated diseases and disorders
PCID1 has potential associations with conditions like neurodegenerative disorders and certain types of cancer. Alterations in its expression or function may disrupt mRNA export impacting cellular function and leading to disease pathogenesis. Proteins like ALYREF and NXF1 connected through these pathways may also exhibit altered activity in such conditions highlighting potential therapeutic targets for treatment development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-PCID1 antibody (ab230105)
All lanes: Western blot - Anti-PCID1 antibody (ab230105) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: A549 (human lung carcinoma cell line) whole cell lysate
Lane 3: Mouse gonadal tissue lysate
Lane 4: Human placenta tissue lysate
Lane 5: Mouse stomach tissue lysate
Lane 6: Mouse brain tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCID1 antibody (ab230105)
Paraffin-embedded human pancreatic cancer tissue stained for PCID1 using ab230105 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-PCID1 antibody (ab230105)
PCID1 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg of ab230105. Western blot was performed from the immunoprecipitate using ab230105. HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 μg control IgG instead of ab230105 in HeLa whole cell lysate .
Lane 2: ab230105 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 μg (Input).All lanes: Immunoprecipitation - Anti-PCID1 antibody (ab230105)
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCID1 antibody (ab230105)
Paraffin-embedded human testis tissue stained for PCID1 using ab230105 at 1/100 dilution in immunohistochemical analysis.
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