AB281938

Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PCIF1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

View Alternative Names

C20orf67, CAPAM, PPP1R121, PCIF1, mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase, Cap-specific adenosine methyltransferase, Phosphorylated CTD-interacting factor 1, Protein phosphatase 1 regulatory subunit 121, hCAPAM, hPCIF1

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Supplier Data

Immunoprecipitation - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

This data was developed using ab271081, the same antibody clone in a different buffer formulation.

PCIF1 was immunoprecipitated from 0.35 mg MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab271081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271081 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab271081 IP in MCF-7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271081 in MCF-7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-PCIF1 antibody [EPR24319-21] (<a href='/en-us/products/primary-antibodies/pcif1-antibody-epr24319-21-ab271081'>ab271081</a>)

Predicted band size: 81 kDa

Observed band size: 85 kDa

false

Lab

Western blot - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

This data was developed using ab271081, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-PCIF1 antibody [EPR24319-21] (<a href='/en-us/products/primary-antibodies/pcif1-antibody-epr24319-21-ab271081'>ab271081</a>) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 81 kDa

Observed band size: 85 kDa

false

Lab

Western blot - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

This data was developed using 271081, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot (lane 3 and 4) was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-PCIF1 antibody [EPR24319-21] (<a href='/en-us/products/primary-antibodies/pcif1-antibody-epr24319-21-ab271081'>ab271081</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 81 kDa

Observed band size: 85 kDa

false

Lab

Western blot - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

This data was developed using ab271081, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-PCIF1 antibody [EPR24319-21] (<a href='/en-us/products/primary-antibodies/pcif1-antibody-epr24319-21-ab271081'>ab271081</a>) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 81 kDa

Observed band size: 85 kDa

false

Unconjugated

Anti-PCIF1 antibody [EPR24319-21]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24319-21

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab281938 is the carrier-free version of ab271081.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCIF1 also known as Phosphorylated CTD-interacting factor 1 is a protein with a mass of around 66 kDa. It functions mechanically as an enzyme that methylates the 2'-O position of RNA cap structures modulating mRNA stability and translation. You find PCIF1 expression predominantly in the nucleus of human cells where it interacts with the C-terminal domain of RNA polymerase II. Its role is essential in RNA processing impacting the regulation of gene expression at a post-transcriptional level.

Biological function summary

PCIF1 modifies RNA molecules influencing mRNA cap structure formation and methylation. This activity is a part of the cap-binding complex which ensures proper RNA maturation and function. By interacting with the cap structure it plays a non-ignorable role in the control of RNA export translation and degradation facilitating the precise regulation of genetic information flow within cells. Its presence in the methyltransferase complex highlights its importance in cell biology.

Pathways

PCIF1 plays a prominent role in the mRNA capping and processing pathways. It partners with proteins such as RNA polymerase II in the pathway to ensure successful transcription and subsequent RNA modifications. The involvement in the mRNA cap methylation pathway highlights its connection with transcription and subsequent mRNA fate marking it as an important regulator in post-transcriptional gene expression regulation.

PCIF1 has been associated with conditions such as cancer and neurodegenerative diseases. Altered expression or function of PCIF1 can disrupt normal cellular processes leading to the progression of these disorders. Studies link PCIF1 changes to irregular mRNA methylation patterns which may relate to oncogenic transformation or neuronal dysfunction. Related proteins like RNA polymerase II also play important roles in understanding these disease mechanisms as they interact within the same pathways disrupted during disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs (PubMed : 30467178, PubMed : 30487554, PubMed : 31279658, PubMed : 31279659, PubMed : 33428944). Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally (PubMed : 30467178).

See full target information PCIF1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free

Immunoprecipitation - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

Western blot - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

Western blot - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

Western blot - Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (AB281938)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise