AB133603

Anti-PCK1/PEPC antibody [EPR6938]

20ul selling size
KO Validated
RabMAb
Recombinant
What is this?

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(5 Publications)

Knockout Tested Rabbit Recombinant Monoclonal PCK1/PEPC antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PEPCK1, PCK1, PEPCK-C, Serine-protein kinase PCK1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling PCK1/PEPC with ab133603 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

Western blot : Anti-PCK1 antibody [EPR6938] (ab133603) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab133603 was shown to bind specifically to PCK1. A band was observed at 67 kDa in wild-type A549 cell lysates with no signal observed at this size in PCK1 knockout cell line. To generate this image, wild-type and PCK1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PCK1/PEPC antibody [EPR6938] (ab133603) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

PCK1 knockout A549 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 67 kDa

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Western blot - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

All lanes:

Western blot - Anti-PCK1/PEPC antibody [EPR6938] (ab133603) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate

Lane 2:

Human adipose tissue lysate

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 39 kDa,69 kDa

Observed band size: 39 kDa,69 kDa

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OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

OI-RD Scanning

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OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6938

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PCK1 also known as phosphoenolpyruvate carboxykinase PEPC or PEPCK plays an important role in gluconeogenesis by converting oxaloacetate to phosphoenolpyruvate (PEP). It has a molecular mass of around 69 kDa. PCK1 is primarily expressed in the liver kidney and adipose tissues. Through its enzymatic activity PCK1 helps to regulate blood glucose levels allowing for the production of glucose from non-carbohydrate sources.

Biological function summary

PCK1 is essential for maintaining glucose homeostasis in the body. Although not part of a larger protein complex its function directly impacts the tricarboxylic acid (TCA) cycle by providing substrates for glucose synthesis. By orchestrating the conversion of oxaloacetate to PEP PCK1 contributes significantly to reducing dependency on dietary carbohydrates particularly during fasting or intensive physical activity.

Pathways

PCK1 integrates critical metabolic processes. It mainly influences the gluconeogenesis and glycolysis pathways. In the gluconeogenesis pathway PCK1 coordinates with enzymes such as glucose-6-phosphatase to facilitate the generation of glucose from lactate and amino acids. Additionally its role in glycolysis intersects with enzymes like pyruvate kinase managing energy production and consumption.

PCK1 links to conditions such as diabetes mellitus and metabolic syndrome. Altered expression or function of PCK1 can lead to disturbances in glucose metabolism. In diabetes dysregulation of PCK1 expression can contribute to hyperglycemia. Additionally PCK1 and its interaction with insulin signaling pathways is critical as insulin resistance is an important feature of metabolic syndrome. Understanding these connections offers potential avenues for therapeutic intervention in metabolic diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed : 24863970, PubMed : 26971250, PubMed : 28216384, PubMed : 30193097). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed : 24863970, PubMed : 26971250, PubMed : 28216384, PubMed : 30193097). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed : 30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed : 30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells : up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (By similarity). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (By similarity). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90 : phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (PubMed : 32322062). The protein kinase activity regulates lipogenesis : upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes (PubMed : 32322062).

See full target information PCK1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 14: PubMed39560969

2024

Tailoring cell therapies for diabetic metabolic phenotypes: a comparative study on the efficacy of various umbilical cord-derived cell regimens.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshu Wang,Liming Wang,Falian He,Jia Song,Jingting Qiao,Jun Qin,Li Chen,Xinguo Hou

BMC cancer 24:611 PubMed38773399

2024

The selective sponging of miRNAs by OIP5-AS1 regulates metabolic reprogramming of pyruvate in adenoma-carcinoma transition of human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Yu Wang,Xiao-Ping Zhang,Hong-Kun Zhou,Hong-Xin Cai,Jin-Biao Xu,Bao-Gang Xie,Jean-Paul Thiery,Wu Zhou

Oncogenesis 12:35 PubMed37407566

2023

MLK4 promotes glucose metabolism in lung adenocarcinoma through CREB-mediated activation of phosphoenolpyruvate carboxykinase and is regulated by KLF5.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Ho-Kwan Cheung,Kit-Yee Wong,Xiaoli Liu,Fenfen Ji,Chris Ho-Lam Hui,Yihan Zhang,Johnny Sheung-Him Kwan,Bonan Chen,Yujuan Dong,Raymond Wai-Ming Lung,Jun Yu,Kwok Wai Lo,Chi Chun Wong,Wei Kang,Ka-Fai To

Stem cell research & therapy 11:223 PubMed32513303

2020

Mesenchymal stem cell-derived exosomes exert ameliorative effects in type 2 diabetes by improving hepatic glucose and lipid metabolism via enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qin He,Lingshu Wang,Ruxing Zhao,Fei Yan,Sha Sha,Chen Cui,Jia Song,Huiqing Hu,Xinghong Guo,Mengmeng Yang,Yixin Cui,Yujing Sun,Zheng Sun,Fuqiang Liu,Ming Dong,Xinguo Hou,Li Chen

Nature communications 8:14420 PubMed28240261

2017

Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Li Bian,Hang-Zi Chen,Peng-Bo Yang,Ying-Ping Li,Fen-Na Zhang,Jia-Yuan Zhang,Wei-Jia Wang,Wen-Xiu Zhao,Sheng Zhang,Qi-Tao Chen,Yu Zheng,Xiao-Yu Sun,Xiao-Min Wang,Kun-Yi Chien,Qiao Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCK1/PEPC antibody [EPR6938]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

Western blot - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

Western blot - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise