Knockout Tested Rabbit Recombinant Monoclonal PCK1/PEPC antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--western-blot-img128259.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95395.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573)")
![OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--oi-rd-scanning-img147922.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573)")
![Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--western-blot-img435379.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (AB248573)")
Publications
Filter by
- Oncology reports 46:2021Quantitative global proteome and phosphorylome analyses reveal potential biomarkers in kidney cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiwei Xu,Zeyi Lu,Shicheng Yu,Gonghui Li,Yuanlei ChenPubMed 34528699
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed:24863970, PubMed:26971250, PubMed:28216384, PubMed:30193097). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed:24863970, PubMed:26971250, PubMed:28216384, PubMed:30193097). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed:30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (By similarity). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (By similarity). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (PubMed:32322062). The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes (PubMed:32322062).
Alternative names
PEPCK1, PCK1, PEPCK-C, Serine-protein kinase PCK1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal PCK1/PEPC antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6938
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 3.5 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248573 is the carrier-free version of Anti-PCK1/PEPC antibody [EPR6938] ab133603.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PCK1 also known as phosphoenolpyruvate carboxykinase PEPC or PEPCK plays an important role in gluconeogenesis by converting oxaloacetate to phosphoenolpyruvate (PEP). It has a molecular mass of around 69 kDa. PCK1 is primarily expressed in the liver kidney and adipose tissues. Through its enzymatic activity PCK1 helps to regulate blood glucose levels allowing for the production of glucose from non-carbohydrate sources.
- Biological function summary
PCK1 is essential for maintaining glucose homeostasis in the body. Although not part of a larger protein complex its function directly impacts the tricarboxylic acid (TCA) cycle by providing substrates for glucose synthesis. By orchestrating the conversion of oxaloacetate to PEP PCK1 contributes significantly to reducing dependency on dietary carbohydrates particularly during fasting or intensive physical activity.
- Pathways
PCK1 integrates critical metabolic processes. It mainly influences the gluconeogenesis and glycolysis pathways. In the gluconeogenesis pathway PCK1 coordinates with enzymes such as glucose-6-phosphatase to facilitate the generation of glucose from lactate and amino acids. Additionally its role in glycolysis intersects with enzymes like pyruvate kinase managing energy production and consumption.
- Associated diseases and disorders
PCK1 links to conditions such as diabetes mellitus and metabolic syndrome. Altered expression or function of PCK1 can lead to disturbances in glucose metabolism. In diabetes dysregulation of PCK1 expression can contribute to hyperglycemia. Additionally PCK1 and its interaction with insulin signaling pathways is critical as insulin resistance is an important feature of metabolic syndrome. Understanding these connections offers potential avenues for therapeutic intervention in metabolic diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--western-blot-img128259.jpg "Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)")
Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)
This data was developed using Anti-PCK1/PEPC antibody [EPR6938] ab133603, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PCK1/PEPC antibody [EPR6938] (Anti-PCK1/PEPC antibody [EPR6938] ab133603) at 1/10000 dilution
Lane 1: Human fetal kidney tissue lysate
Lane 2: Human adipose tissue lysate
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa, 69 kDa
Observed band size: 39 kDa, 69 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95395.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)
This data was developed using Anti-PCK1/PEPC antibody [EPR6938] ab133603, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling PCK1/PEPC with Anti-PCK1/PEPC antibody [EPR6938] ab133603 at 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--oi-rd-scanning-img147922.jpg "OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)")
OI-RD Scanning - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573), expandable thumbnail](https://content.abcam.com/products/images/pck1-pepc-antibody-epr6938-bsa-and-azide-free-ab248573--western-blot-img435379.jpg "Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)")
Western blot - Anti-PCK1/PEPC antibody [EPR6938] - BSA and Azide free (ab248573)
This data was developed using the same antibody clone in a different buffer formulation (abAB133603).
Western blot: Anti-PCK1 antibody [EPR6938] (Anti-PCK1/PEPC antibody [EPR6938] ab133603) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-PCK1/PEPC antibody [EPR6938] ab133603 was shown to bind specifically to PCK1. A band was observed at 67 kDa in wild-type A549 cell lysates with no signal observed at this size in PCK1 knockout cell line. To generate this image, wild-type and PCK1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PCK1/PEPC antibody [EPR6938] (Anti-PCK1/PEPC antibody [EPR6938] ab133603) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: PCK1 knockout A549 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: HEK-293 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 67 kDa
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