AB137580

Anti-PCK2 antibody

Name: Anti-PCK2 antibody (ab137580) | Abcam
Brand: Abcam Limited
SKU: AB137580

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(4 Publications)

Rabbit Polyclonal PCK2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCK2 aa 1 to C-terminus.

View Alternative Names

PEPCK2, PCK2, PEPCK-M, Serine/threonine-protein kinase PCK2, mtPCK2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody (AB137580)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody (AB137580)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling PCK2 with ab137580 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody (AB137580)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody (AB137580)

Immunofluorescence analysis of HepG2 cells fixed in MeOH for 5 min labelling PCK2 protein at mitochondria using ab137580 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Supplier Data

Western blot - Anti-PCK2 antibody (AB137580)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PCK2 antibody (ab137580) at 1/500 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

HeLa whole cell lysates at 30 µg

Lane 3:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 71 kDa

false

Unknown

Western blot - Anti-PCK2 antibody (AB137580)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PCK2 antibody (ab137580) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Unknown

Western blot - Anti-PCK2 antibody (AB137580)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PCK2 antibody (ab137580) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PCK2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16822

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoenolpyruvate carboxykinase 2 (PCK2) also known as mitochondrial PEPCK is an enzyme that plays an important role in gluconeogenesis. It catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the mitochondria. PCK2 has a molecular mass of approximately 69 kDa and is expressed mainly in tissues that require high energy production such as the liver and kidney. Despite its vital function in energy metabolism PCK2 is also present in other metabolic tissues.

Biological function summary

PCK2 contributes to the regulation of glucose homeostasis. It achieves this by being a part of the gluconeogenic pathway working in concert with cytosolic PCK1. PCK2 does not work alone; it integrates into a larger metabolic framework that includes various enzymes essential for converting different substrates into glucose. This enzyme is notably active during fasting states or periods of high energy demands such as intense exercise ensuring a continuous supply of glucose to maintain cellular functions.

Pathways

PCK2 plays an important role in the gluconeogenesis and TCA cycle. The gluconeogenesis pathway allows the liver and kidney to produce glucose from non-carbohydrate substrates which is integral during fasting and low-carbohydrate diet. It is functionally linked with other key enzymes like pyruvate carboxylase in converting pyruvate to glucose and also cooperates with malate dehydrogenase in the TCA cycle. This cooperation ensures efficient energy production and conversion balance in the cell.

PCK2 expression is linked to type 2 diabetes and certain cancers. In type 2 diabetes altered PCK2 activity impacts glucose production contributing to hyperglycemia. Studies have shown that its dysregulation may correlate with insulin resistance a characteristic feature of diabetes. In cancer particularly some forms of breast cancer the interplay between PCK2 and oncogenes like KRAS can affect tumor metabolism promoting the adaptation of cancer cells to the energy-demanding environment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed : 28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed : 28955899).

See full target information PCK2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 12:1363-1373 PubMed34532094

2021

AKT inhibits the phosphorylation level of H2A at Tyr57 via CK2α to promote the progression of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Da Chen,Peng-Fei Zhang,Hong-Qing Xi,Bo Wei,Lin Chen

Molecular oncology : PubMed32777161

2020

Distribution and prognostic significance of gluconeogenesis and glycolysis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Smolle,Petra Leko,Elvira Stacher-Priehse,Luka Brcic,Amin El-Heliebi,Lilli Hofmann,Franz Quehenberger,Andelko Hrzenjak,Helmut H Popper,Horst Olschewski,Katharina Leithner

Thyroid : official journal of the American Thyroid Association 29:845-857 PubMed30990120

2019

Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie Strickaert,Cyril Corbet,Selim-Alex Spinette,Ligia Craciun,Geneviève Dom,Guy Andry,Denis Larsimont,Ruddy Wattiez,Jacques E Dumont,Olivier Feron,Carine Maenhaut

Oncogene 34:1044-50 PubMed24632615

2014

PCK2 activation mediates an adaptive response to glucose depletion in lung cancer.

Applications

Unspecified application

Species

Human

K Leithner,A Hrzenjak,M Trötzmüller,T Moustafa,H C Köfeler,C Wohlkoenig,E Stacher,J Lindenmann,A L Harris,A Olschewski,H Olschewski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody (AB137580)

Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody (AB137580)

Western blot - Anti-PCK2 antibody (AB137580)

Western blot - Anti-PCK2 antibody (AB137580)

Western blot - Anti-PCK2 antibody (AB137580)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise