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AB187145

Anti-PCK2 antibody [EPR14224] - C-terminal

4

(1 Review)

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(8 Publications)

Rabbit Recombinant Monoclonal PCK2 antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

PEPCK2, PCK2, PEPCK-M, Serine/threonine-protein kinase PCK2, mtPCK2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells, labeling PCK2 (green) with ab187145 at 1/500 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling PCK2 with ab187145 at 1/500 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

Intracellular Flow Cytometry analysis of PCK2 expression in 2% paraformaldehyde-fixed HeLa cells using ab187145 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)
  • WB

Supplier Data

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

All lanes:

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/10000 dilution

All lanes:

HepG2 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 71 kDa

false

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)
  • WB

Supplier Data

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

All lanes:

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 71 kDa

false

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)
  • WB

Supplier Data

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (AB187145)

All lanes:

Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution

Lane 1:

MCF7 lysate at 20 µg

Lane 2:

Hela lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 71 kDa

false

  • Carrier free

    Anti-PCK2 antibody [EPR14224] - BSA and Azide free

  • 660 APC

    APC Anti-PCK2 antibody [EPR14224] (C-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PCK2 antibody [EPR14224] - C-terminal

  • HRP

    HRP Anti-PCK2 antibody [EPR14224] (C-terminal)

  • 578 PE

    PE Anti-PCK2 antibody [EPR14224] (C-terminal)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14224

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoenolpyruvate carboxykinase 2 (PCK2) also known as mitochondrial PEPCK is an enzyme that plays an important role in gluconeogenesis. It catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the mitochondria. PCK2 has a molecular mass of approximately 69 kDa and is expressed mainly in tissues that require high energy production such as the liver and kidney. Despite its vital function in energy metabolism PCK2 is also present in other metabolic tissues.
Biological function summary

PCK2 contributes to the regulation of glucose homeostasis. It achieves this by being a part of the gluconeogenic pathway working in concert with cytosolic PCK1. PCK2 does not work alone; it integrates into a larger metabolic framework that includes various enzymes essential for converting different substrates into glucose. This enzyme is notably active during fasting states or periods of high energy demands such as intense exercise ensuring a continuous supply of glucose to maintain cellular functions.

Pathways

PCK2 plays an important role in the gluconeogenesis and TCA cycle. The gluconeogenesis pathway allows the liver and kidney to produce glucose from non-carbohydrate substrates which is integral during fasting and low-carbohydrate diet. It is functionally linked with other key enzymes like pyruvate carboxylase in converting pyruvate to glucose and also cooperates with malate dehydrogenase in the TCA cycle. This cooperation ensures efficient energy production and conversion balance in the cell.

PCK2 expression is linked to type 2 diabetes and certain cancers. In type 2 diabetes altered PCK2 activity impacts glucose production contributing to hyperglycemia. Studies have shown that its dysregulation may correlate with insulin resistance a characteristic feature of diabetes. In cancer particularly some forms of breast cancer the interplay between PCK2 and oncogenes like KRAS can affect tumor metabolism promoting the adaptation of cancer cells to the energy-demanding environment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed : 28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed : 28955899).
See full target information PCK2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:14051 PubMed38890507

2024

Association of mitochondrial phosphoenolpyruvate carboxykinase with prognosis and immune regulation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxuan Li,En-di Zhang,Youzhi Ye,Zhongyun Xiao,Hanfei Huang,Zhong Zeng

Oncogenesis 12:35 PubMed37407566

2023

MLK4 promotes glucose metabolism in lung adenocarcinoma through CREB-mediated activation of phosphoenolpyruvate carboxykinase and is regulated by KLF5.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Ho-Kwan Cheung,Kit-Yee Wong,Xiaoli Liu,Fenfen Ji,Chris Ho-Lam Hui,Yihan Zhang,Johnny Sheung-Him Kwan,Bonan Chen,Yujuan Dong,Raymond Wai-Ming Lung,Jun Yu,Kwok Wai Lo,Chi Chun Wong,Wei Kang,Ka-Fai To

Chinese herbal medicines 14:97-103 PubMed36120128

2022

Anti-diabetic effects of linarin from via AMPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenji Wang,Zhe Bai,Jinghua Yan,Tengteng Liu,Yingmei Li,Jiahui Xu,Xiaoqian Meng,Yuefeng Bi

Free radical biology & medicine 176:34-45 PubMed34520823

2021

PCK2 opposes mitochondrial respiration and maintains the redox balance in starved lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Bluemel,Mélanie Planque,Corina T Madreiter-Sokolowski,Theresa Haitzmann,Andelko Hrzenjak,Wolfgang F Graier,Sarah-Maria Fendt,Horst Olschewski,Katharina Leithner

Journal of extracellular vesicles 10:e12026 PubMed33304478

2020

The proteomic landscape of small urinary extracellular vesicles during kidney transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Braun,Markus Rinschen,Denise Buchner,Katrin Bohl,Ingo Plagmann,Daniel Bachurski,Martin Richard Späth,Philipp Antczak,Heike Göbel,Corinna Klein,Jan-Wilm Lackmann,Oliver Kretz,Victor G Puelles,Roger Wahba,Michael Hallek,Bernhard Schermer,Thomas Benzing,Tobias B Huber,Andreas Beyer,Dirk Stippel,Christine E Kurschat,Roman-Ulrich Müller

Molecular oncology : PubMed32777161

2020

Distribution and prognostic significance of gluconeogenesis and glycolysis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Smolle,Petra Leko,Elvira Stacher-Priehse,Luka Brcic,Amin El-Heliebi,Lilli Hofmann,Franz Quehenberger,Andelko Hrzenjak,Helmut H Popper,Horst Olschewski,Katharina Leithner

The Journal of biological chemistry 295:3891-3905 PubMed32051143

2020

Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhang,Zhe Shen,Yiming Lin,Jie Zhang,Yuwei Zhang,Peihao Liu,Hang Zeng,Mengli Yu,Xueyang Chen,Longgui Ning,Xinli Mao,Li Cen,Chaohui Yu,Chengfu Xu

Journal of cellular and molecular medicine 23:5895-5906 PubMed31287224

2019

MicroRNA-351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hong Chen,Xiao-Nan Liu,Yan Peng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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