AB72443

Anti-PCM1 antibody

Name: Anti-PCM1 antibody (ab72443) | Abcam
Brand: Abcam Limited
SKU: AB72443
Price: 525 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(9 Publications)

Rabbit Polyclonal PCM1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human PCM1.

View Alternative Names

Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1

2 Images

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Immunoprecipitation - Anti-PCM1 antibody (AB72443)

Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded) :
Lane 1 : PCM1 precipitate

Lane 2 : Control IgG

For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PCM1 antibody (ab72443)

Predicted band size: 229 kDa

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Western blot - Anti-PCM1 antibody (AB72443)

Detection : Chemiluminescence with exposure times of 30 seconds

All lanes:

Western blot - Anti-PCM1 antibody (ab72443) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 229 kDa

Observed band size: 270 kDa,60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PCM1. The exact immunogen used to generate this antibody is proprietary information.

Q15154

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCM1 (Pericentriolar Material 1) also known as the PCM-1 protein functions mechanically in the organization of centrosomes and microtubules. This mechanistic role is linked with its name due to its positioning around the centrosome a critical cellular structure for division. The PCM1 protein shows an approximate mass of 228 kDa. It is expressed broadly in various tissues but mainly in organs with high cellular turnover including the testis thymus and heart. Notably PCM1 serves as a scaffold protein anchoring various centrosomal proteins important for their correct assembly and positioning.

Biological function summary

The PCM1 protein plays an important role in the microtubule stabilization and cell cycle progression. This protein becomes part of the larger pericentriolar material complex which supports the necessary structural platform for centrosome and cilia function. PCM1 assists in docking other proteins like centrin centrin-binding proteins and pericentriolar satellites in the complex ensuring the intricacies of ciliary trafficking and centrosome cohesion are well-maintained. It helps in regulating processes critical to normal cell polarity and division.

Pathways

PCM1 protein integrates pathway activities relating to not only microtubule dynamism but also cellular motility. It is involved in the centrosome-cilia interface important for signal transduction and cellular homeostasis. PCM1 interacts with key pathway proteins such as CEP290 and OFD1 which are significant for ciliogenesis thereby influencing cellular responses to extracellular cues. These pathways directly tie the mechanics of PCM1 to essential biological responses including cell signaling pathways that ensure normal embryonic development and cell differentiation.

PCM1 plays a relevant role in the context of ciliopathies and certain cancers. Abnormalities or mutations in PCM1 have associations with disorders such as Bardet-Biedl Syndrome a ciliopathy characterized by malfunctioning cilia. Aberrant PCM1 expressions or mutations also have links to leukemia through its implication in the ETV6-PCM1 fusion gene. The interplay between PCM1 and proteins such as ETV6 in leukemia highlights its impact on chromosomal translocations and the potential muscular pathway misregulation leading to malignancy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for centrosome assembly and function (PubMed : 12403812, PubMed : 15659651, PubMed : 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed : 12403812, PubMed : 15659651). Required to anchor microtubules to the centrosome (PubMed : 12403812, PubMed : 15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed : 20551181, PubMed : 24121310, PubMed : 27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed : 34782749).

See full target information PCM1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115091 PubMed39709602

2024

Transcriptional profile of the rat cardiovascular system at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Arduini,Stephen J Fleming,Ling Xiao,Amelia W Hall,Amer-Denis Akkad,Mark D Chaffin,Kayla J Bendinelli,Nathan R Tucker,Irinna Papangeli,Helene Mantineo,Patricio Flores-Bringas,Mehrtash Babadi,Christian M Stegmann,Guillermo García-Cardeña,Mark E Lindsay,Carla Klattenhoff,Patrick T Ellinor

iScience 27:109580 PubMed38600973

2024

Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Clotilde C N Renaud,Carolina Alves Nicolau,Clément Maghe,Kilian Trillet,Jane Jardine,Sophie Escot,Nicolas David,Julie Gavard,Nicolas Bidère

Communications biology 6:1271 PubMed38102401

2023

The centrosomal protein 131 participates in the regulation of mitochondrial apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Clotilde C N Renaud,Kilian Trillet,Jane Jardine,Laura Merlet,Ophélie Renoult,Mélanie Laurent-Blond,Zoé Catinaud,Claire Pecqueur,Julie Gavard,Nicolas Bidère

Cell reports 28:1907-1922.e6 PubMed31412255

2019

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kari-Anne M Frikstad,Elisa Molinari,Marianne Thoresen,Simon A Ramsbottom,Frances Hughes,Stef J F Letteboer,Sania Gilani,Kay O Schink,Trond Stokke,Stefan Geimer,Lotte B Pedersen,Rachel H Giles,Anna Akhmanova,Ronald Roepman,John A Sayer,Sebastian Patzke

Molecular biology of the cell 29:1542-1554 PubMed29742019

2018

The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Robert F Shearer,Kari-Anne Myrum Frikstad,Jessie McKenna,Rachael A McCloy,Niantao Deng,Andrew Burgess,Trond Stokke,Sebastian Patzke,Darren N Saunders

Nature communications 8:14177 PubMed28134340

2017

KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth B Schou,Johanne B Mogensen,Stine K Morthorst,Brian S Nielsen,Aiste Aleliunaite,Andrea Serra-Marques,Nicoline Fürstenberg,Sophie Saunier,Albane A Bizet,Iben R Veland,Anna Akhmanova,Søren T Christensen,Lotte B Pedersen

eLife 4: PubMed26386247

2015

TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).

Applications

ICC

Species

Unspecified reactive species

Louise A Stephen,Hasan Tawamie,Gemma M Davis,Lars Tebbe,Peter Nürnberg,Gudrun Nürnberg,Holger Thiele,Michaela Thoenes,Eugen Boltshauser,Steffen Uebe,Oliver Rompel,André Reis,Arif B Ekici,Lynn McTeir,Amy M Fraser,Emma A Hall,Pleasantine Mill,Nicolas Daudet,Courtney Cross,Uwe Wolfrum,Rami Abou Jamra,Megan G Davey,Hanno J Bolz

Cell cycle (Georgetown, Tex.) 14:1116-25 PubMed25830415

2015

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ankur A Gholkar,Silvia Senese,Yu-Chen Lo,Joseph Capri,William J Deardorff,Harish Dharmarajan,Ely Contreras,Emmanuelle Hodara,Julian P Whitelegge,Peter K Jackson,Jorge Z Torres

Nature communications 5:5308 PubMed25374274

2014

Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents.

Applications

Species

Unspecified reactive species

Jantje M Gerdes,Sonia Christou-Savina,Yan Xiong,Tilo Moede,Noah Moruzzi,Patrick Karlsson-Edlund,Barbara Leibiger,Ingo B Leibiger,Claes-Göran Östenson,Philip L Beales,Per-Olof Berggren

View all publications

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Product promise

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Anti-PCM1 antibody

Immunoprecipitation - Anti-PCM1 antibody (AB72443)

Western blot - Anti-PCM1 antibody (AB72443)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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