AB192790

Anti-PCMT1 antibody

Name: Anti-PCMT1 antibody (ab192790) | Abcam
Brand: Abcam Limited
SKU: AB192790

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(2 Publications)

Rabbit Polyclonal PCMT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCMT1 aa 1-250.

View Alternative Names

Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, PCMT1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PCMT1 antibody (AB192790)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PCMT1 antibody (AB192790)

Immunofluorescent analysis of methanol fixed A431 cells labeling PCMT1 with ab192790 at 1/200 (green). The cells were costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCMT1 antibody (AB192790)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCMT1 antibody (AB192790)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling PCMT1 with an192790 at 7.5 μg/m.

Supplier Data

Western blot - Anti-PCMT1 antibody (AB192790)

12% SDS PAGE

All lanes:

Western blot - Anti-PCMT1 antibody (ab192790) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PCMT1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P22061

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "7.5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PCMT1 protein also known as protein-L-isoaspartate (D-aspartate) O-methyltransferase catalyzes the repair of damaged proteins by recognizing and converting abnormal L-isoaspartyl residues to normal L-aspartyl residues. This enzymatic activity is important for maintaining protein integrity and function. The purified form of PCMT1 has a mass of approximately 24 kDa. It is ubiquitously expressed in various tissues with high levels found in the liver and brain emphasizing its essential role across different body systems.

Biological function summary

The PCMT1 enzyme plays a significant role in cellular repair mechanisms by rejuvenating proteins that have been altered due to spontaneous deamidation or isomerization. This process is critical for preserving the longevity of proteins and preventing cellular aging and dysfunction. PCMT1 does not generally form part of a complex but works by interacting directly with protein substrates that contain abnormal isoaspartyl residues. It functions independently in its activity and this specificity highlights its importance in maintaining cellular homeostasis.

Pathways

PCMT1 is an integral component of protein repair pathways specifically the pathways that manage protein maintenance and degradation. These pathways help prevent the accumulation of dysfunctional proteins thereby preserving cell function and survival. PCMT1 activity relates closely to proteins such as CHIP and ubiquitin-proteasome pathway elements which also manage protein quality control within the cell. Together these proteins ensure accurate protein folding and turnover.

PCMT1 activity connects to neurodegenerative disorders like Alzheimer's disease and demyelinating conditions such as multiple sclerosis. The deficiency or dysfunction in PCMT1 could lead to abnormal accumulation of damaged proteins causing cellular stress and contributing to disease pathology. In the context of these conditions PCMT1 has also been linked to other proteins like beta-amyloid in Alzheimer's illustrating the cascading effect that modified protein repair can have on disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed : 3167043, PubMed : 6469980). Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

See full target information PCMT1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:4478-4492 PubMed39525569

2024

Charge-guided masking of a membrane-destabilizing peptide enables efficient endosomal escape for targeted intracellular delivery of proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Haolin Jiang,Hang Chen,Jiazhen Yu,Luyao Wang,Wen Zhou,Juanjuan Du

Frontiers in endocrinology 10:769 PubMed31798532

2019

PKA Activates AMPK Through LKB1 Signaling in Follicular Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Kari,Vasyl V Vasko,Shivam Priya,Lawrence S Kirschner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCMT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PCMT1 antibody (AB192790)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCMT1 antibody (AB192790)

Western blot - Anti-PCMT1 antibody (AB192790)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise