Rabbit Polyclonal PCMT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCMT1 aa 1-200.
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Publications
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- Frontiers in genetics 11:6123432021l-Isoaspartyl Methyltransferase Deficiency in Zebrafish Leads to Impaired Calcium Signaling in the Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRemon Soliman,Maria Lorena Cordero-Maldonado,Teresa G Martins,Mahsa Moein,Jean-François Conrotte,Rebeccah A Warmack,Alexander Skupin,Alexander D Crawford,Steven G Clarke,Carole L LinsterPubMed 33552132
- Cancer science 109:3794-38042018Forkhead box C1 boosts triple-negative breast cancer metastasis through activating the transcription of chemokine receptor-4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongchao Pan et. al.PubMed 30290049
- Oncotarget 9:13287-133002018Deficiency of protein-L-isoaspartate (D-aspartate) -methyl-transferase expression under endoplasmic reticulum stress promotes epithelial mesenchymal transition in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Yamashita et. al.PubMed 29568357
- Human pathology 50:1-102016Chaperone protein L-isoaspartate (D-aspartyl) O-methyltransferase as a novel predictor of poor prognosis in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeisuke Saito,Masahiro Yamashita,Masahito Ogasawara,Noriyuki Yamada,Miyuki Niisato,Makoto Tomoyasu,Hiroyuki Deguchi,Tatsuo Tanita,Kazuyuki Ishida,Tamotsu Sugai,Kohei YamauchiPubMed 26997432
- Scientific reports 4:66742014Methylation of histone H4 at aspartate 24 by protein L-isoaspartate O-methyltransferase (PCMT1) links histone modifications with protein homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBurcu Biterge,Florian Richter,Gerhard Mittler,Robert SchneiderPubMed 25327473
- International journal of cardiology 168:3291-92013Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PCMT1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P22061
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed:3167043, PubMed:6469980). Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).
Alternative names
Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, PCMT1
Recommended products
Rabbit Polyclonal PCMT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCMT1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PCMT1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P22061
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PCMT1 protein also known as protein-L-isoaspartate (D-aspartate) O-methyltransferase catalyzes the repair of damaged proteins by recognizing and converting abnormal L-isoaspartyl residues to normal L-aspartyl residues. This enzymatic activity is important for maintaining protein integrity and function. The purified form of PCMT1 has a mass of approximately 24 kDa. It is ubiquitously expressed in various tissues with high levels found in the liver and brain emphasizing its essential role across different body systems.
- Biological function summary
The PCMT1 enzyme plays a significant role in cellular repair mechanisms by rejuvenating proteins that have been altered due to spontaneous deamidation or isomerization. This process is critical for preserving the longevity of proteins and preventing cellular aging and dysfunction. PCMT1 does not generally form part of a complex but works by interacting directly with protein substrates that contain abnormal isoaspartyl residues. It functions independently in its activity and this specificity highlights its importance in maintaining cellular homeostasis.
- Pathways
PCMT1 is an integral component of protein repair pathways specifically the pathways that manage protein maintenance and degradation. These pathways help prevent the accumulation of dysfunctional proteins thereby preserving cell function and survival. PCMT1 activity relates closely to proteins such as CHIP and ubiquitin-proteasome pathway elements which also manage protein quality control within the cell. Together these proteins ensure accurate protein folding and turnover.
- Associated diseases and disorders
PCMT1 activity connects to neurodegenerative disorders like Alzheimer's disease and demyelinating conditions such as multiple sclerosis. The deficiency or dysfunction in PCMT1 could lead to abnormal accumulation of damaged proteins causing cellular stress and contributing to disease pathology. In the context of these conditions PCMT1 has also been linked to other proteins like beta-amyloid in Alzheimer's illustrating the cascading effect that modified protein repair can have on disease development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PCMT1 antibody (ab97446)
12% SDS PAGE
All lanes: Western blot - Anti-PCMT1 antibody (ab97446) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCMT1 antibody (ab97446)
Immunohistochemical analysis of PCMT1 in paraffin embedded U87 xenograft, using ab97446 at a 1/500 dilution.
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