AB252848

Anti-PCNA antibody [16D10]

Recombinant
Lab Essentials
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(10 Publications)

Rat Recombinant Monoclonal PCNA antibody. Suitable for ICC/IF, IP and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

View Alternative Names

DNA sliding clamp PCNA, Cyclin, Proliferating cell nuclear antigen, PCNA

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

Immunocytochemical analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2μg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10μg/ml) followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (2μg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterdstain was DAPI (Blue).

-ve control 1 : ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

-ve control 2 : ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

Immunocytochemical analysis of 100% methanol-fixed C6 (rat glial tumor glial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2μg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10μg/ml) followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (2μg/ml) (Red). Confocal image showing nuclear staining in C6 cell line. The nuclear counterdstain was DAPI (Blue).

-ve control 1 : ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

-ve control 2 : ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

Immunocytochemical analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2μg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10μg/ml) followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (2μg/ml) (Red). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterdstain was DAPI (Blue).

-ve control 1 : ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

-ve control 2 : ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

Supplier Data

Immunoprecipitation - Anti-PCNA antibody [16D10] (AB252848)

PCNA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate using ab252848 at 1/60 dilution (2μg in 0.35μg lysates). Western blot was performed on the immunoprecipitate using ab252848 at 1/1000 dilution (0.35μg/ml) followed by anti-Rat IgG H&L (HRP) (ab205720) secondary at 1/5000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg.

Lane 2 : ab252848 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rat monoclonal IgG instead of ab252848 in NIH/3T3 whole cell lysate.

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-PCNA antibody [16D10] (ab252848)

Predicted band size: 29 kDa

false

Carrier free

Anti-PCNA antibody [16D10] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

16D10

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Confers DNA tethering and processivity to DNA polymerases and other proteins (PubMed : 24695737, PubMed : 24939902, PubMed : 35585232). Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).

See full target information PCNA

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7799 PubMed40921755

2025

The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Kyohei Matsuhashi,Kei K Ito,Kaho Nagai,Akira Sanada,Koki Watanabe,Kasuga Takumi,Atsushi Toyoda,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Grant S Stewart,Shoji Hata,Daiju Kitagawa

The EMBO journal 44:1294-1321 PubMed39905228

2025

Multimodal mechanisms of human centriole engagement and disengagement.

Applications

Unspecified application

Species

Unspecified reactive species

Kei K Ito,Kasuga Takumi,Kyohei Matsuhashi,Hirokazu Sakamoto,Kaho Nagai,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Shoji Hata,Daiju Kitagawa

Science signaling 17:eadk4122 PubMed39689183

2024

Castration-resistant prostate cancer is resensitized to androgen deprivation by autophagy-dependent apoptosis induced by blocking SKP2.

Applications

Unspecified application

Species

Unspecified reactive species

Sherly I Celada,Guoliang Li,Lindsay J Celada,Thanigaivelan Kanagasabai,Wenfu Lu,LaKendria K Brown,Zaniya A Mark,Michael G Izban,Billy R Ballard,Xinchun Zhou,Samuel E Adunyah,Robert J Matusik,Xiaofei Wang,Zhenbang Chen

Drug development research 85:e70033 PubMed39660541

2024

Heme Oxygenase-1 Overexpression Activates the IRF1/DRP1 Signaling Pathway to Promote M2-Type Polarization of Spinal Cord Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Wenping Lin,Ziming Cai,Jinzhu Liang,Ping Miao,Ye Ruan,Pian Li,Shuhui Lin,He Tian,Qinghe Yu,Xu He

Journal of cellular physiology 239:e31248 PubMed38501506

2024

Naringin activates semaphorin 3A to ameliorate TGF-β-induced endothelial-to-mesenchymal transition related to atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ju Lai,Shang-Hung Chang,Ying-Chang Tung,Gwo-Jyh Chang,Celina De Almeida,Wei-Jan Chen,Yung-Hsin Yeh,Feng-Chun Tsai

The EMBO journal 43:1015-1042 PubMed38360994

2024

PARG-deficient tumor cells have an increased dependence on EXO1/FEN1-mediated DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Andronikou,Kamila Burdova,Diego Dibitetto,Cor Lieftink,Elke Malzer,Hendrik J Kuiken,Ewa Gogola,Arnab Ray Chaudhuri,Roderick L Beijersbergen,Hana Hanzlikova,Jos Jonkers,Sven Rottenberg

Nature 621:602-609 PubMed37704733

2023

A vertebral skeletal stem cell lineage driving metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Lingling Hu,Seoyeon Bok,Alisha R Yallowitz,Michelle Cung,Jason McCormick,Ling J Zheng,Shawon Debnath,Yuzhe Niu,Adrian Y Tan,Sarfaraz Lalani,Kyle W Morse,Daniel Shinn,Anthony Pajak,Mohammed Hammad,Vincentius Jeremy Suhardi,Zan Li,Na Li,Lijun Wang,Weiguo Zou,Vivek Mittal,Mathias P G Bostrom,Ren Xu,Sravisht Iyer,Matthew B Greenblatt

Physiological reports 11:e15549 PubMed36597221

2023

β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Keyvan Karimi Galougahi,Yunjia Zhang,Vivian Kienzle,Chia-Chi Liu,Lake-Ee Quek,Sanjay Patel,Edmund Lau,Rachael L Cordina,Gemma A Figtree,David S Celermajer

Nature structural & molecular biology 29:329-338 PubMed35332322

2022

PARP inhibition impedes the maturation of nascent DNA strands during DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Vaitsiankova,Kamila Burdova,Margarita Sobol,Amit Gautam,Oldrich Benada,Hana Hanzlikova,Keith W Caldecott

The Journal of cell biology 176:565-71 PubMed17312023

2007

DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Spada,Andrea Haemmer,David Kuch,Ulrich Rothbauer,Lothar Schermelleh,Elisabeth Kremmer,Thomas Carell,Gernot Längst,Heinrich Leonhardt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCNA antibody [16D10]

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] (AB252848)

Immunoprecipitation - Anti-PCNA antibody [16D10] (AB252848)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise