Anti-PCNA antibody [16D10] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (AB255842)

Immunocytochemical analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2μg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10μg/ml) followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (2μg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterdstain was DAPI (Blue).

-ve control 1 : ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

-ve control 2 : ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (AB255842)

Immunocytochemical analysis of 100% methanol-fixed C6 (rat glial tumor glial cell) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2μg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10μg/ml) followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (2μg/ml) (Red). Confocal image showing nuclear staining in C6 cell line. The nuclear counterdstain was DAPI (Blue).

-ve control 1 : ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

-ve control 2 : ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [16D10] - BSA and Azide free (AB255842)

Immunocytochemical analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling PCNA (green) using ab252848 at 1/100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2μg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10μg/ml) followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (2μg/ml) (Red). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterdstain was DAPI (Blue).

-ve control 1 : ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

-ve control 2 : ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848).

• IP

Supplier Data

Immunoprecipitation - Anti-PCNA antibody [16D10] - BSA and Azide free (AB255842)

PCNA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate using ab252848 at 1/60 dilution (2μg in 0.35μg lysates). Western blot was performed on the immunoprecipitate using ab252848 at 1/1000 dilution (0.35μg/ml) followed by anti-Rat IgG H&L (HRP) (ab205720) secondary at 1/5000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg.

Lane 2 : ab252848 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rat monoclonal IgG instead of ab252848 in NIH/3T3 whole cell lysate.

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848).

All lanes:

Immunoprecipitation - Anti-PCNA antibody [16D10] (<a href='/en-us/products/primary-antibodies/pcna-antibody-16d10-ab252848'>ab252848</a>)

Predicted band size: 29 kDa

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