Anti-PCNA antibody [24/PCNA]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PCNA with ab280088 at 1/5000 (0.215 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on human colon.

The section was incubated with ab280088 for 30 mins at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling PCNA with ab280088 at 1/20 (53.7 μg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in HeLa cell line.

ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab280088 at a 1/20 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PCNA with ab280088 at 1/5000 (0.215 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Nuclear staining on human tonsil.

The section was incubated with ab280088 for 30 minutes at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling PCNA with ab280088 at 1/20 (53.7 μg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in NIH/3T3 cell line.

ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab280088 at a 1/20 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PCNA with ab280088 at 1/5000 (0.215 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on mouse spleen.

The section was incubated with ab280088 for 30 minutes at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 cells labelling PCNA with ab280088 at 1/20 (53.7 μg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear and weak cytoplasmic staining in C6 cell line.

ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab280088 at a 1/20 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (AB280088)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PCNA with ab280088 at 1/5000 (0.215 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on rat spleen.

The section was incubated with ab280088 for 30 minutes at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.

• WB

Supplier Data

Western blot - Anti-PCNA antibody [24/PCNA] (AB280088)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PCNA antibody [24/PCNA] (ab280088) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-PCNA antibody [24/PCNA] (AB280088)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PCNA antibody [24/PCNA] (ab280088) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

false

Exposure time: 6s