AB70472

Anti-PCNA antibody [5E6/2]

Name: Anti-PCNA antibody [5E6/2] (ab70472) | Abcam
Brand: Abcam Limited
SKU: AB70472
Rating: 1 (1 reviews)

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What is this?

(1 Review)

(18 Publications)

Mouse Monoclonal PCNA antibody. Suitable for WB, IP, ELISA and reacts with Saccharomyces cerevisiae samples. Cited in 18 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30.

View Alternative Names

YBR088C, YBR0811, POL30, DNA sliding clamp PCNA, Proliferating cell nuclear antigen, PCNA

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E6/2

Isotype

IgG1

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

WB, IP, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30.

P15873

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Confers DNA tethering and processivity to DNA polymerases and other proteins (PubMed : 9343398, PubMed : 11545742, PubMed : 12226657, PubMed : 23747975). Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand (PubMed : 9343398, PubMed : 11545742). Additionally, acts as a molecular switch to control the choice of two parallel branches of the DNA damage tolerance (DDT) pathway (also known as the post-replication repair (PRR) pathway) that bypasses replication-blocking lesions without removing them; the translesion DNA synthesis (TLS) branch, and the template-switching (TS) branch; monoubiquitinated PCNA promotes the TLS branch and polyubiquitinated PCNA promotes the TS branch (PubMed : 12226657, PubMed : 23747975). Within TLS, it can either directly recruit REV1 to stressed replication forks, or indirectly following RAD5 recruitment (PubMed : 37594077).

See full target information POL30

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10496 PubMed39627214

2024

ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yeongjae Kim,Na Young Ha,Mi-Sun Kang,Eunjin Ryu,Geunil Yi,Juyeong Yoo,Nalae Kang,Byung-Gyu Kim,Kyungjae Myung,Sukhyun Kang

mBio 14:e0031523 PubMed36861970

2023

Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.

Applications

Unspecified application

Species

Unspecified reactive species

Li Fan,Wenqing Zhang,Josephine Rybchuk,Yu Luo,Wei Xiao

PLoS biology 20:e3001940 PubMed36574440

2022

The RNA export and RNA decay complexes THO and TRAMP prevent transcription-replication conflicts, DNA breaks, and CAG repeat contractions.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca E Brown,Xiaofeng A Su,Stacey Fair,Katherine Wu,Lauren Verra,Robyn Jong,Kristin Andrykovich,Catherine H Freudenreich

mBio 13:e0142022 PubMed35708277

2022

S. cerevisiae Cells Can Grow without the Pds5 Cohesin Subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Karan Choudhary,Ziv Itzkovich,Elisa Alonso-Perez,Hend Bishara,Barbara Dunn,Gavin Sherlock,Martin Kupiec

Current biology : CB : PubMed33417883

2021

Ligation of newly replicated DNA controls the timing of DNA mismatch repair.

Applications

Unspecified application

Species

Unspecified reactive species

Gloria X Reyes,Anna Kolodziejczak,Lovely Jael Paul Solomon Devakumar,Takashi Kubota,Richard D Kolodner,Christopher D Putnam,Hans Hombauer

Nature 586:623-627 PubMed32814343

2020

PCNA activates the MutLγ endonuclease to promote meiotic crossing over.

Applications

Unspecified application

Species

Unspecified reactive species

Dhananjaya S Kulkarni,Shannon N Owens,Masayoshi Honda,Masaru Ito,Ye Yang,Mary W Corrigan,Lan Chen,Aric L Quan,Neil Hunter

Nature structural & molecular biology 27:450-460 PubMed32341533

2020

Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas A Guilliam,Joseph T P Yeeles

Nucleic acids research 47:6826-6841 PubMed31114918

2019

Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Lovely Jael Paul Solomon Devakumar,Christl Gaubitz,Victoria Lundblad,Brian A Kelch,Takashi Kubota

PLoS genetics 14:e1007783 PubMed30418970

2018

Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation.

Applications

Unspecified application

Species

Unspecified reactive species

Vamsi K Gali,David Dickerson,Yuki Katou,Katsunori Fujiki,Katsuhiko Shirahige,Tom Owen-Hughes,Takashi Kubota,Anne D Donaldson

Oncology reports 41:377-386 PubMed30365139

2018

miR‑101 regulates the cell proliferation and apoptosis in diffuse large B‑cell lymphoma by targeting MEK1 via regulation of the ERK/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Huang,Yong Zou,Luhui Lin,Xudong Ma,Ruiji Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCNA antibody [5E6/2]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise