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AB152112

Anti-PCNA antibody

3

(2 Reviews)

|

(37 Publications)

Rabbit Polyclonal PCNA antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Rat, Human, Drosophila melanogaster samples. Cited in 37 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCNA aa 1 to C-terminus.

View Alternative Names

Proliferating cell nuclear antigen, PCNA, Cyclin

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (AB152112)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (AB152112)

Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells labelling PCNA protein at nucleus using ab152112 at a 1/500 dilution at RT for 15 min (Green). Phalloidin, a cytoskeleton marker was used to counterstain Phalloidin diluted at 1/200 dilution (Red).
Scale bar = 10 µm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB152112)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB152112)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (AB152112)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (AB152112)

Immunofluorescence analysis of ice-cold MeOH fixed HeLa cells labelling PCNA protein at nucleus using ab152112 at a 1/1000 dilution at for 5 min (Green). The nuclear counter stain is Hoechst 33342 (Blue).
Scale bar= 10 µm.

Western blot - Anti-PCNA antibody (AB152112)
  • WB

Supplier Data

Western blot - Anti-PCNA antibody (AB152112)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-PCNA antibody (ab152112) at 1/2500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Predicted band size: 29 kDa

false

Western blot - Anti-PCNA antibody (AB152112)
  • WB

Supplier Data

Western blot - Anti-PCNA antibody (AB152112)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-PCNA antibody (ab152112) at 1/2500 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

false

Western blot - Anti-PCNA antibody (AB152112)
  • WB

Supplier Data

Western blot - Anti-PCNA antibody (AB152112)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-PCNA antibody (ab152112) at 1/2500 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB152112)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB152112)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB152112)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB152112)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.

Immunoprecipitation - Anti-PCNA antibody (AB152112)
  • IP

Supplier Data

Immunoprecipitation - Anti-PCNA antibody (AB152112)

PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 μg ab152112. Western blot was performed from the immunoprecipitate using ab152112.

Lane 1 : PCPB2 protein only.

Lane 2 : IgG instead of ab152112 in HeLa whole cell extract.

Lane 3 : ab152112 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-PCNA antibody (ab152112)

Predicted band size: 29 kDa

false

Western blot - Anti-PCNA antibody (AB152112)
  • WB

Supplier Data

Western blot - Anti-PCNA antibody (AB152112)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-PCNA antibody (ab152112) at 1/1000 dilution

All lanes:

S2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Drosophila melanogaster

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human PCNA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P12004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).
See full target information PCNA
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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

BMC microbiology 25:117 PubMed40038589

2025

Comparative in silico and in vivo study of the antioxidant activity of lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis against Rotavirus infection in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tarek A M Ismail,Naglaa Elshafey,Shehab E Talat,Mona E Saif,Amany Mohammed Hegab,Komla Mawunyo Dossouvi,Hanan M Alharbi,Amr Elkelish,Khalid Abd El Ghany

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 35:933-944 PubMed39641310

2024

Long Non-Coding RNA SNHG3 Promotes the Progression of Cholangiocarcinoma by Regulating the miR-151a-3p/STAT5a Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Wei,Dongyun Cun,Danping Yang,Qianyao Yi,Daguang Tian

European journal of clinical investigation 54:e14212 PubMed38591651

2024

Elevated circulating BMP9 aggravates pulmonary angiogenesis in hepatopulmonary syndrome rats through ALK1-Endoglin-Smad1/5/9 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyong Yang,Mei Sun,Yihui Yang,Yan Han,Xiulin Wu,Xianfeng Wu,Huilin Cao,Lin Chen,Yuhao Lei,Xiaoyan Hu,Yang Chen,Ziyang Zeng,Junhong Li,Xin Shu,Zhiyong Yang,Kaizhi Lu,Yujie Li,Xiaobo Wang,Bin Yi

Thoracic cancer 14:1543-1555 PubMed37073425

2023

Downregulation of circ_PLXND1 inhibits tumorigenesis of non-small cell lung carcinoma via miR-1287-5p/ERBB3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinzhou Wu,Chenyang Liu,Guiping Yu

Translational oncology 32:101661 PubMed37037089

2023

LncRNA DGUOK-AS1 facilitates non-small cell lung cancer growth and metastasis through increasing TRPM7 stability via m6A modification.

Applications

Unspecified application

Species

Unspecified reactive species

Yimin Feng,Fengjuan Wu,Yuanning Wu,Zihan Guo,Xiang Ji

Inflammopharmacology 31:1341-1359 PubMed37010718

2023

Diosmin nanocrystal gel alleviates imiquimod-induced psoriasis in rats via modulating TLR7,8/NF-κB/micro RNA-31, AKT/mTOR/P70S6K milieu, and Tregs/Th17 balance.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmine Shahine,Sarah A Abd El-Aal,Ahmed M Reda,Eman Sheta,Nouran M Atia,Ossama Y Abdallah,Sherihan Salaheldin Abdelhamid Ibrahim

Antioxidants (Basel, Switzerland) 12: PubMed36829854

2023

Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Heise,Thomas Schmitz,Christoph Bührer,Stefanie Endesfelder

Clinical and experimental pharmacology & physiology 49:1105-1115 PubMed35748299

2022

Circ_0017274 acts on miR-637/CDX2 axis to facilitate cisplatin resistance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Xu,Jinjuan Guo,Mei Chen

Thoracic cancer 13:1276-1288 PubMed35348291

2022

Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Jin,Fengping Zhang,Qiubo Li,Ruiqi Xu,Ying Liu,Yong Zhang

Cell death discovery 8:132 PubMed35338123

2022

MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Ge Zhao,Weiwei Li
View all publications
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