Rabbit Polyclonal PCNA antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Rat, Human, Drosophila melanogaster samples. Cited in 30 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCNA aa 1 to C-terminus.
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- Inflammopharmacology 31:1341-13592023Diosmin nanocrystal gel alleviates imiquimod-induced psoriasis in rats via modulating TLR7,8/NF-κB/micro RNA-31, AKT/mTOR/P70S6K milieu, and Tregs/Th17 balance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasmine Shahine,Sarah A Abd El-Aal,Ahmed M Reda,Eman Sheta,Nouran M Atia,Ossama Y Abdallah,Sherihan Salaheldin Abdelhamid IbrahimPubMed 37010718
- Antioxidants (Basel, Switzerland) 12:2023Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Heise,Thomas Schmitz,Christoph Bührer,Stefanie EndesfelderPubMed 36829854
- Clinical and experimental pharmacology & physiology 49:1105-11152022Circ_0017274 acts on miR-637/CDX2 axis to facilitate cisplatin resistance in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Xu,Jinjuan Guo,Mei ChenPubMed 35748299
- Thoracic cancer 13:1276-12882022Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Jin,Fengping Zhang,Qiubo Li,Ruiqi Xu,Ying Liu,Yong ZhangPubMed 35348291
- Cell death discovery 8:1322022MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhao,Ge Zhao,Weiwei LiPubMed 35338123
- Frontiers in cellular neuroscience 15:6510722021GABA Receptor-Mediated Impairment of Intermediate Progenitor Maturation During Postnatal Hippocampal Neurogenesis of Newborn Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Gustorff,Till Scheuer,Thomas Schmitz,Christoph Bührer,Stefanie EndesfelderPubMed 34421540
- Frontiers in cell and developmental biology 9:6556282021CircRNA DOCK1 Regulates miR-409-3p/MCL1 Axis to Modulate Proliferation and Apoptosis of Human Brain Vascular Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinmin Ding,Xiaolong Wang,Li Han,Zhiyu Zhao,Shuai Jia,Yuanzhao TuoPubMed 34109173
- Frontiers in cell and developmental biology 8:6227932021Nuclear Factor-κB Activating Protein Plays an Oncogenic Role in Neuroblastoma Tumorigenesis and Recurrence Through the Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Liu,Mingyu Zhang,Ying Kan,Wei Wang,Jie Liu,Jianhua Gong,Jigang YangPubMed 33553160
- Neoplasma 68:391-4032021CircRNA hsa_circ_0030018 regulates the development of glioma via regulating the miR-1297/RAB21 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDewen Song,Lin Ye,Zengliang Xu,Yanfei Jin,Lintao ZhangPubMed 33440987
- Oncology letters 20:3792020Notch3 is involved in the proliferation of renal cancer cells via regulation of cell cycle progression and HIF-2α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQipeng Han,Fangzhu Han,Yisheng Fan,Bowen Lian,Jinyang Xiao,Wei Sun,Dongbing Han,Hongbo Kou,Chunyan Li,Bin WuPubMed 33154777
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PCNA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12004
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Expected |
| Rat | Tested | Expected | Tested | Expected |
| Drosophila melanogaster | Expected | Expected | Tested | Expected |
| Pig | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/1000 | Notes Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Drosophila melanogaster | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Recommended products
Rabbit Polyclonal PCNA antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Rat, Human, Drosophila melanogaster samples. Cited in 30 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCNA aa 1 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PCNA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12004
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
- Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
- Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
- Associated diseases and disorders
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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18 product images
Immunoprecipitation - Anti-PCNA antibody (ab152112)
PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 μg ab152112. Western blot was performed from the immunoprecipitate using ab152112.
Lane 1: PCPB2 protein only.
Lane 2: IgG instead of ab152112 in HeLa whole cell extract.
Lane 3: ab152112 IP in HeLa whole cell extract.
All lanes: Immunoprecipitation - Anti-PCNA antibody (ab152112)
Predicted band size: 29 kDa
Western blot - Anti-PCNA antibody (ab152112)
12% SDS PAGE
All lanes: Western blot - Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1: HCT116 whole cell lysate at 15 µg
Lane 2: HCT116 whole cell lysate at 10 µg
Lane 3: HCT116 whole cell lysate at 5 µg
Lane 4: HCT116 whole cell lysate at 1 µg
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Paraffin-embedded mouseliver tissue stained for PCNA using ab152112 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue labeling PCNA with ab152112 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Immunohistochemical analysis of paraffin-embedded Rat hind brain tissue labeling PCNA with ab152112 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PCNA with ab152112 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PCNA with ab152112 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)
Immunofluorescent analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling PCNA with ab152112 at 1/500 dilution.
Counterstain is Hoechst 33342 (blue).
Western blot - Anti-PCNA antibody (ab152112)
12% SDS PAGE
All lanes: Western blot - Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: 293T whole cell lysate at 30 µg
Lane 4: A431 whole cell lysate at 30 µg
Lane 5: HeLa whole cell lyate at 30 µg
Lane 6: HepG2 whole cell lysate at 30 µg
Lane 7: H1299 whole cell lysate at 30 µg
Lane 8: HCT116 whole cell lysate at 30 µg
Lane 9: MCF7 whole cell lysate at 30 µg
Lane 10: NT2D1 whole cell lysate at 30 µg
Lane 11: PC3 whole cell lysate at 30 µg
Lane 12: U87 MG whole cell lysate at 30 µg
Predicted band size: 29 kDa
Western blot - Anti-PCNA antibody (ab152112)
12% SDS PAGE
All lanes: Western blot - Anti-PCNA antibody (ab152112) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL1 whole cell lysate at 30 µg
Predicted band size: 29 kDa
Western blot - Anti-PCNA antibody (ab152112)
12% SDS PAGE
All lanes: Western blot - Anti-PCNA antibody (ab152112) at 1/1000 dilution
All lanes: PC12 whole cell lysate at 30 µg
Predicted band size: 29 kDa
Western blot - Anti-PCNA antibody (ab152112)
12% SDS PAGE
All lanes: Western blot - Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: NIH 3T3 whole cell lysate at 30 µg
Lane 3: PC12 whole cell lysate at 30 µg
Predicted band size: 29 kDa
Western blot - Anti-PCNA antibody (ab152112)
12% SDS PAGE
All lanes: Western blot - Anti-PCNA antibody (ab152112) at 1/1000 dilution
All lanes: NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 29 kDa, 40 kDa, 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)
Immunofluorescence analysis of ice-cold MeOH fixed HeLa cells labelling PCNA protein at nucleus using ab152112 at a 1/1000 dilution at for 5 min (Green). The nuclear counter stain is Hoechst 33342 (Blue).
Scale bar= 10 µm.
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)
Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells labelling PCNA protein at nucleus using ab152112 at a 1/500 dilution at RT for 15 min (Green). Phalloidin, a cytoskeleton marker was used to counterstain Phalloidin diluted at 1/200 dilution (Red).
Scale bar = 10 µm.
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