Rabbit Polyclonal PCNA antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 14 publications.
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- International journal of molecular sciences 24:2023Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Hwan Kim,Eui Hwa Jang,Ji-Yeon Ryu,Jiyong Lee,Jae Ho Kim,Wonhyoung Ryu,Young-Nam YounPubMed 36834717
- American journal of translational research 12:6464-64772020Erythropoietin enhances meniscal regeneration and prevents osteoarthritis formation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Ning Fu et. al.PubMed 33194044
- Experimental and therapeutic medicine 20:1672020Glutamate receptor ionotropic, kainate 1 serves as a novel tumor suppressor of colorectal carcinoma and predicts clinical prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong Ren et. al.PubMed 33093905
- Bioscience reports 40:2020Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui-Hua Zhang,Fa-An Miao,Jin-Ge Xu,Yan ZhangPubMed 32969473
- Thoracic cancer 11:1180-11902020Long non-coding RNA SNHG6 promotes the growth and invasion of non-small cell lung cancer by downregulating miR-101-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Li et. al.PubMed 32147945
- RNA biology 16:1401-14132019Long non-coding RNA slincRAD functions in methylation regulation during the early stage of mouse adipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yi et. al.PubMed 31199203
- Frontiers in cell and developmental biology 7:562019Glucocorticoids Target Ependymal Glia and Inhibit Repair of the Injured Spinal Cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCraig M Nelson et. al.PubMed 31069223
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:254-2632018Classic SRY-box protein SOX7 functions as a tumor suppressor regulating WNT signaling and is methylated in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Wang,Yu Fan,Lian Zhang,Lili Li,Guanyu Kuang,Cheng Luo,Chen Li,Tingxiu Xiang,Qian Tao,Qian Zhang,Jianming YingPubMed 29957056
- Molecular cell 70:395-407.e42018The BUB3-BUB1 Complex Promotes Telomere DNA Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Li,Hyeung Kim,Zhejian Ji,Tianpeng Zhang,Bohong Chen,Yuanlong Ge,Yang Hu,Xuyang Feng,Xin Han,Huimin Xu,Youwei Zhang,Hongtao Yu,Dan Liu,Wenbin Ma,Zhou SongyangPubMed 29727616
- Molecular medicine reports 17:1683-16892017Local honokiol application inhibits intimal thickening in rabbits following carotid artery balloon injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang,Danyang Zhao,Jing Sheng,Ping LuPubMed 29257208
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Alligator | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Turkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Alligator, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1 µg/mL | Notes - |
Species Human | Dilution info 0.1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Alligator, Orangutan | Dilution info - | Notes - |
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Target data
Function
Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin
Recommended products
Rabbit Polyclonal PCNA antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 14 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
- Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
- Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
- Associated diseases and disorders
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PCNA antibody (ab19166)
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Western blot - Anti-PCNA antibody (ab19166) at 0.1 µg/mL
Lane 1: HEK293T whole cell lysate at 50 µg
Lane 2: NIH-3T3 whole cell lysate at 50 µg
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab19166)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling PCNA with ab19166 at 1/10000 (0.1µg/ml). Detection: DAB.
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