AB221196

Anti-PCNA antibody

Lab Essentials
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal PCNA antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30.

View Alternative Names

YBR088C, YBR0811, POL30, DNA sliding clamp PCNA, Proliferating cell nuclear antigen, PCNA

2 Images

Supplier Data

Western blot - Anti-PCNA antibody (AB221196)

All lanes:

Western blot - Anti-PCNA antibody (ab221196) at 1/1000 dilution

All lanes:

Crude extract of Saccharomyces cerevisiae strain BY4741 at 20 µg

Predicted band size: 29 kDa

false

CiteAb

Western blot - Anti-PCNA antibody (AB221196)

PCNA western blot using anti-PCNA antibody ab221196. Publication image and figure legend from Shor, E., Garcia-Rubio, R., et al., 2020, MBio, PubMed 33323516.

ab221196 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab221196 please see the product overview.

Expression of PCNA is upregulated by DNA damage in S. cerevisiae but downregulated in C. glabrata. (A) Heatmaps of genes involved in maintenance of genome stability and differentially regulated by DNA damage in S. cerevisiae and C. glabrata. (B) PCNA protein levels increase in response to DNA damage in S. cerevisiae but not in C. glabrata. Cells were treated by 0.1% MMS by 2 h and then harvested for total cell lysates and Western blot. Tubulin, alpha-tubulin. (C) Quantification of Western blot data from at least three biological replicates (Image J). For every condition, PCNA abundance (in arbitrary units [A.U.]) was normalized to that of alpha-tubulin.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30.

P15873

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Confers DNA tethering and processivity to DNA polymerases and other proteins (PubMed : 9343398, PubMed : 11545742, PubMed : 12226657, PubMed : 23747975). Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand (PubMed : 9343398, PubMed : 11545742). Additionally, acts as a molecular switch to control the choice of two parallel branches of the DNA damage tolerance (DDT) pathway (also known as the post-replication repair (PRR) pathway) that bypasses replication-blocking lesions without removing them; the translesion DNA synthesis (TLS) branch, and the template-switching (TS) branch; monoubiquitinated PCNA promotes the TLS branch and polyubiquitinated PCNA promotes the TS branch (PubMed : 12226657, PubMed : 23747975). Within TLS, it can either directly recruit REV1 to stressed replication forks, or indirectly following RAD5 recruitment (PubMed : 37594077).

See full target information POL30

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:16731 PubMed37794081

2023

Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlin S Radmall,Prakash K Shukla,Andrew M Leng,Mahesh B Chandrasekharan

mBio 11: PubMed33323516

2020

A Noncanonical DNA Damage Checkpoint Response in a Major Fungal Pathogen.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Shor,Rocio Garcia-Rubio,Lucius DeGregorio,David S Perlin

Genetics 213:449-463 PubMed31451562

2019

Mutations in the PCNA DNA Polymerase Clamp of Reveal Complexities of the Cell Cycle and Ploidy on Heterochromatin Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Molly Brothers,Jasper Rine

Proceedings of the National Academy of Sciences of 115:E2030-E2039 PubMed29440488

2018

Pivotal roles of PCNA loading and unloading in heterochromatin function.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan Janke,Grant A King,Martin Kupiec,Jasper Rine

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCNA antibody

Western blot - Anti-PCNA antibody (AB221196)

Western blot - Anti-PCNA antibody (AB221196)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise