Rabbit Polyclonal PCNA antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30.
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- Scientific reports 13:167312023Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaitlin S Radmall,Prakash K Shukla,Andrew M Leng,Mahesh B ChandrasekharanPubMed 37794081
- mBio 11:2020A Noncanonical DNA Damage Checkpoint Response in a Major Fungal Pathogen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErika Shor et. al.PubMed 33323516
- Genetics 213:449-4632019Mutations in the PCNA DNA Polymerase Clamp of Reveal Complexities of the Cell Cycle and Ploidy on Heterochromatin Assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMolly Brothers et. al.PubMed 31451562
- Proceedings of the National Academy of Sciences of 115:E2030-E20392018Pivotal roles of PCNA loading and unloading in heterochromatin function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan Janke et. al.PubMed 29440488
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30. Database link P15873
Reactivity data
Select an application| WB | |
|---|---|
| Saccharomyces cerevisiae | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 1/1000 | Notes - |
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Target data
Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.
Alternative names
YBR088C, YBR0811, POL30, Proliferating cell nuclear antigen, PCNA
Recommended products
Rabbit Polyclonal PCNA antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C POL30. Database link P15873
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
- Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
- Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
- Associated diseases and disorders
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PCNA antibody (ab221196)
All lanes: Western blot - Anti-PCNA antibody (ab221196) at 1/1000 dilution
All lanes: Crude extract of Saccharomyces cerevisiae strain BY4741 at 20 µg
Predicted band size: 29 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-PCNA antibody (ab221196)
PCNA western blot using anti-PCNA antibody ab221196. Publication image and figure legend from Shor, E., Garcia-Rubio, R., et al., 2020, MBio, PubMed 33323516.
ab221196 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab221196 please see the product overview.
Expression of PCNA is upregulated by DNA damage in S. cerevisiae but downregulated in C. glabrata. (A) Heatmaps of genes involved in maintenance of genome stability and differentially regulated by DNA damage in S. cerevisiae and C. glabrata. (B) PCNA protein levels increase in response to DNA damage in S. cerevisiae but not in C. glabrata. Cells were treated by 0.1% MMS by 2 h and then harvested for total cell lysates and Western blot. Tubulin, alpha-tubulin. (C) Quantification of Western blot data from at least three biological replicates (Image J). For every condition, PCNA abundance (in arbitrary units [A.U.]) was normalized to that of alpha-tubulin.
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