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AB265609

Anti-PCNA antibody [BLR075G]

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(12 Publications)

Rabbit Recombinant Monoclonal PCNA antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PCNA aa 200 to C-terminus.

View Alternative Names

DNA sliding clamp PCNA, Cyclin, Proliferating cell nuclear antigen, PCNA

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (AB265609)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (AB265609)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (AB265609)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (AB265609)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection : DAB.

Immunocytochemistry - Anti-PCNA antibody [BLR075G] (AB265609)
  • ICC

Supplier Data

Immunocytochemistry - Anti-PCNA antibody [BLR075G] (AB265609)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling PCNA with ab265609 at 1/250 dilution. Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (AB265609)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] (AB265609)

Paraffin embedded human breast carcinoma tissue stained for PCNA using ab265609 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used HRP-conjugated goat anti-rabbit IgG. Counterstain : DAPI.

Western blot - Anti-PCNA antibody [BLR075G] (AB265609)
  • WB

Supplier Data

Western blot - Anti-PCNA antibody [BLR075G] (AB265609)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PCNA antibody [BLR075G] (ab265609) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 50 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

RKO whole cell lysate at 50 µg

Lane 5:

K562 whole cell lysate at 50 µg

Lane 6:

MCF7 whole cell lysate at 50 µg

Lane 7:

HeLa whole cell lysate at 50 µg

Lane 8:

U-2 OS whole cell lysate at 50 µg

Lane 9:

TCMK whole cell lysate at 50 µg

Lane 10:

NIH/3T3 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 29 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR075G

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human PCNA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P12004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Confers DNA tethering and processivity to DNA polymerases and other proteins (PubMed : 24695737, PubMed : 24939902, PubMed : 35585232). Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).
See full target information PCNA
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:4902 PubMed39929852

2025

ESM1 promote proliferation, invasion and angiogenesis via Akt/mTOR and Ras pathway in kidney renal clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Luo,Ting Yi,Yong Wang,Wei Song,Zhiyong Gao,Jiansong Wang,Yukun Li

Heliyon 10:e36897 PubMed39281564

2024

The role of ESM1 in the lipids metabolic reprogramming and angiogenesis of lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchang Feng,Yi Ting,Xing Tang,Dan Liu,Wen-Chao Zhou,Yukun Li,Zhenyu Shen

Molecular cancer 23:94 PubMed38720298

2024

ESM1 enhances fatty acid synthesis and vascular mimicry in ovarian cancer by utilizing the PKM2-dependent warburg effect within the hypoxic tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Zhang,Fan Ouyang,Anbo Gao,Tian Zeng,Ming Li,Hui Li,Wenchao Zhou,Qing Gao,Xing Tang,Qunfeng Zhang,Xiaomin Ran,Gang Tian,Xiyun Quan,Zhenzi Tang,Juan Zou,Yifei Zeng,Yunzhu Long,Yukun Li

Journal of translational medicine 22:46 PubMed38212795

2024

ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Kun Li,An-Bo Gao,Tian Zeng,Dan Liu,Qun-Feng Zhang,Xiao-Min Ran,Zhen-Zi Tang,Yan Li,Jue Liu,Ting Zhang,Gang-Qing Shi,Wen-Chao Zhou,Wen-da Zou,Juan Peng,Juan Zhang,Hui Li,Juan Zou

Aging 16:8348-8360 PubMed38159259

2023

ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Qihao Hu,Shi Chen,Yukun Li,Teng Hu,Jianpeng Hu,Cheng Wang,Fei Yang,Xiang Yang,Feng Zhou,Zhengdong Liu,Wei Xu,Ji Zhang

International journal of biological sciences 19:258-280 PubMed36594088

2023

Validation of ESM1 Related to Ovarian Cancer and the Biological Function and Prognostic Significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Kun Li,Tian Zeng,Yang Guan,Jue Liu,Nian-Chun Liao,Meng-Jie Wang,Ke-Xin Chen,Xian-Yu Luo,Chang-Ye Chen,Fei-Fei Quan,Juan Wang,Qun-Feng Zhang,Juan Zou

Journal of oncology 2022:5026308 PubMed36213820

2022

Influence of Dehydroxymethylepoxyquinomicin on Radiosensitivity of Thyroid Carcinoma TPC-1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Hu Cai,Heqing Yi,Xin Li,Yunsong Peng,Linfa Li

Journal of atherosclerosis and thrombosis 29:1849-1863 PubMed36171087

2022

Oxygenized Low-Density Lipoprotein-Induced ASMC Dysregulation Depends on circ_0000345-Mediated Regulatory Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Song Chen,Lixiu Sun,Jingjing Zhang,Ling Zhang,Xian Liu

Frontiers in cell and developmental biology 9:682261 PubMed34249934

2021

Immunity-Related Gene Signature Identifies Subtypes Benefitting From Adjuvant Chemotherapy or Potentially Responding to PD1/PD-L1 Blockage in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Qian,Hongzhe Li,Junjie Xie,Xiongxiong Lu,Fanlu Li,Weishen Wang,Xiaomei Tang,Minmin Shi,Linxi Jiang,Hongwei Li,Hao Chen,Chenghong Peng,Zhiwei Xu,Xiaxing Deng,Baiyong Shen

Frontiers in cell and developmental biology 9:653207 PubMed34012964

2021

Circular RNA Circ0021205 Promotes Cholangiocarcinoma Progression Through MiR-204-5p/RAB22A Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfei Tu,Weiqian Chen,Liyun Zheng,Shiji Fang,Dengke Zhang,Chunli Kong,Yang Yang,Rongfang Qiu,Zhongwei Zhao,Chenying Lu,Xiaojie Lu,Jiansong Ji
View all publications
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