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Rabbit Recombinant Monoclonal PCNA antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PCNA aa 200 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (AB272076), expandable thumbnail
  • Immunocytochemistry - Anti-PCNA antibody [BLR075G] - BSA and Azide free (AB272076), expandable thumbnail
  • Western blot - Anti-PCNA antibody [BLR075G] - BSA and Azide free (AB272076), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (AB272076), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (AB272076), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human PCNA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12004

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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      Target data

      Function

      Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

      Alternative names

      Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

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      Rabbit Recombinant Monoclonal PCNA antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PCNA aa 200 to C-terminus.

      Alternative names

      Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • Synthetic Peptide within Human PCNA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12004
      Clone number

      BLR075G

      Purification technique

      Affinity purification

      Concentration
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      Purification notes

      Purified from TCS.

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      ab272076 is the BSA-free version of Anti-PCNA antibody [BLR075G] ab265609.

      This product is sold under License from Bethyl Laboratories, Inc.

      Supplementary info

      Activity summary

      PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

      Biological function summary

      This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

      Pathways

      PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

      Associated diseases and disorders

      PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
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      5 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PCNA with Anti-PCNA antibody [BLR075G] ab265609 at 1/250 dilution. Detection: DAB.

        This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-PCNA antibody [BLR075G] ab265609).

      • Immunocytochemistry - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076), expandable thumbnail

        Immunocytochemistry - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)

        Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling PCNA with Anti-PCNA antibody [BLR075G] ab265609 at 1/250 dilution. Detection: DAB.

        This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-PCNA antibody [BLR075G] ab265609).

      • Western blot - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076), expandable thumbnail

        Western blot - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)

        Detection: Chemiluminescence.

        This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-PCNA antibody [BLR075G] ab265609).

        Lysates prepared using NETN lysis buffer.

        All lanes: Western blot - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076) at 1/1000 dilution

        Lane 1: A549 whole cell lysate at 50 µg

        Lane 2: Jurkat whole cell lysate at 50 µg

        Lane 3: HEK-293T whole cell lysate at 50 µg

        Lane 4: RKO whole cell lysate at 50 µg

        Lane 5: K562 whole cell lysate at 50 µg

        Lane 6: MCF7 whole cell lysate at 50 µg

        Lane 7: HeLa whole cell lysate at 50 µg

        Lane 8: U-2 OS whole cell lysate at 50 µg

        Lane 9: TCMK whole cell lysate at 50 µg

        Lane 10: NIH/3T3 whole cell lysate at 50 µg

        Secondary

        All lanes: HRP-conjugated goat anti-rabbit IgG

        Predicted band size: 29 kDa

        Exposure time: 30s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling PCNA with Anti-PCNA antibody [BLR075G] ab265609 at 1/250 dilution. Detection: DAB.

        This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-PCNA antibody [BLR075G] ab265609).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [BLR075G] - BSA and Azide free (ab272076)

        This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-PCNA antibody [BLR075G] ab265609).

        Paraffin embedded human breast carcinoma tissue stained for PCNA using Anti-PCNA antibody [BLR075G] ab265609 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used HRP-conjugated goat anti-rabbit IgG. Counterstain: DAPI.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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