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Chicken Recombinant Monoclonal PCNA antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (AB322919), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (AB322919), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (AB322919), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (AB322919), expandable thumbnail

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

Alternative names

Proliferating cell nuclear antigen, PCNA, Cyclin

Chicken Recombinant Monoclonal PCNA antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3821
Purification technique
Affinity purification Thiophilic Resin
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab322919 is the carrier free version of Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-PCNA antibody [EPR3821] ab92552). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919)

    PCNA Immunocytochemistry/ Immunofluorescence staining of A-431 cells using chicken Anti-PCNA antibody

    Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083 staining PCNA in A-431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083 at 1µg/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This data was developed using the same antibody clone in a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919)

    PCNA Immunocytochemistry/ Immunofluorescence staining of NIH/3T3 cells using chicken Anti-PCNA antibody

    Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083 staining PCNA in NIH/3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083 at 1µg/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This data was developed using the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919)

    PCNA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon using chicken Anti-PCNA antibody

    Immunofluorescence staining of PCNA in sections of formalin-fixed paraffin-embedded human colon*.
    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083 at 1/100 dilution and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. This data was developed using the same antibody clone in a different buffer formulation.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] – Chicken IgY (Chimeric) – BSA and Azide Free (ab322919)

    PCNA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using chicken Anti-PCNA antibody

    Immunofluorescence staining of PCNA in sections of formalin-fixed paraffin-embedded mouse testis.
    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-PCNA [EPR3821] – Chicken IgY (Chimeric) ab322083 at 1/500 dilution and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. This data was developed using the same antibody clone in a different buffer formulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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