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Rabbit Monoclonal PCNA antibody. Carrier free. Suitable for WB and reacts with Human, Dog, Cat, Zebrafish, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PCNA.

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Images

Western blot - Anti-PCNA antibody [HL1965] (AB308212), expandable thumbnail
  • Western blot - Anti-PCNA antibody [HL1965] (AB308212), expandable thumbnail
  • Western blot - Anti-PCNA antibody [HL1965] (AB308212), expandable thumbnail
  • Western blot - Anti-PCNA antibody [HL1965] (AB308212), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human PCNA. The exact immunogen used to generate this antibody is proprietary information. Database link P12004

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
Cat
Tested
Dog
Tested
Zebrafish
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Dog

Dilution info

1/1000

Notes

-

Species

Cat

Dilution info

1/1000

Notes

-

Species

Zebrafish

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Target data

Function

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

Alternative names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Recommended products

Rabbit Monoclonal PCNA antibody. Carrier free. Suitable for WB and reacts with Human, Dog, Cat, Zebrafish, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PCNA.

Alternative names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human PCNA. The exact immunogen used to generate this antibody is proprietary information. Database link P12004
Carrier free

Yes

Clone number

HL1965

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

Associated diseases and disorders

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PCNA antibody [HL1965] (ab308212), expandable thumbnail

    Western blot - Anti-PCNA antibody [HL1965] (ab308212)

    Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody [HL1965] (ab308212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Whole zebrafish extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-PCNA antibody [HL1965] (ab308212), expandable thumbnail

    Western blot - Anti-PCNA antibody [HL1965] (ab308212)

    Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody [HL1965] (ab308212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: Neuro2A cell lysate at 30 µg

    Lane 2: Rat2 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-PCNA antibody [HL1965] (ab308212), expandable thumbnail

    Western blot - Anti-PCNA antibody [HL1965] (ab308212)

    Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody [HL1965] (ab308212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: MDCK cell lysate at 30 µg

    Lane 2: PG-4 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-PCNA antibody [HL1965] (ab308212), expandable thumbnail

    Western blot - Anti-PCNA antibody [HL1965] (ab308212)

    Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody [HL1965] (ab308212) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

    Lane 1: HeLa cell lysate at 30 µg

    Lane 2: HepG2 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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