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Mouse Monoclonal PCNA antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Pcna.

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Images

Immunoprecipitation - Anti-PCNA antibody [PC10] (AB265585), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (AB265585), expandable thumbnail
  • Western blot - Anti-PCNA antibody [PC10] (AB265585), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (AB265585), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (AB265585), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Rat Pcna. Database link P04961

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt
Human
Expected
Expected
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

-

Notes

20 μl/1 mg lysate

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/500.00000

Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µL for 106 Cells

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

Alternative names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Recommended products

Mouse Monoclonal PCNA antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Pcna.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Rat Pcna. Database link P04961
Clone number

PC10

Purification technique

Affinity purification

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

Associated diseases and disorders

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

Product promise

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6 product images

  • Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585), expandable thumbnail

    Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585)

    PCNA was immunoprecipitated from 1 mg TCMK-1 cell lysate using ab265585 at 20 μl/mg lysate. Western blot was performed on the immunoprecipitate using ab265585 at 1/1000 dilution.

    Lane 1: ab265585 IP in TCMK-1 whole cell lysate.
    Lane 2: Control IgG.

    All lanes: Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585)

    Developed using the ECL technique.

    Predicted band size: 29 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB

  • Western blot - Anti-PCNA antibody [PC10] (ab265585), expandable thumbnail

    Western blot - Anti-PCNA antibody [PC10] (ab265585)

    All lanes: Western blot - Anti-PCNA antibody [PC10] (ab265585) at 1/1000 dilution

    Lane 1: TCMK-1 whole cell lysate at 50 µg

    Lane 2: NIH/3T3 whole cell lysate at 50 µg

    Lane 3: HeLa whole cell lysate at 50 µg

    Lane 4: Jurkat whole cell lysate at 50 µg

    Secondary

    All lanes: HRP conjugated goat anti-mouse IgG

    Developed using the ECL technique.

    Predicted band size: 29 kDa

    Exposure time: 1s

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab265585), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab265585)

    Formalin-fixed, paraffin-embedded H226 cells stained for PCNA using ab265585 at 1/100 dilution in ICC/IF analysis. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)

    Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB

  • Flow Cytometry - Anti-PCNA antibody [PC10] (ab265585), expandable thumbnail

    Flow Cytometry - Anti-PCNA antibody [PC10] (ab265585)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling PCNA with ab265585 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details, DyLight® 488-conjugated goat anti-mouse IgG (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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