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AB265585

Anti-PCNA antibody [PC10]

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(3 Publications)

Mouse Monoclonal PCNA antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Pcna.

View Alternative Names

DNA sliding clamp PCNA, Cyclin, Proliferating cell nuclear antigen, PCNA

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (AB265585)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (AB265585)

Formalin-fixed, paraffin-embedded H226 cells stained for PCNA using ab265585 at 1/100 dilution in ICC/IF analysis. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (AB265585)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (AB265585)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate : DAB

Flow Cytometry - Anti-PCNA antibody [PC10] (AB265585)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-PCNA antibody [PC10] (AB265585)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling PCNA with ab265585 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details, DyLight® 488-conjugated goat anti-mouse IgG (ab96879).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (AB265585)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (AB265585)

Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate : DAB

Immunoprecipitation - Anti-PCNA antibody [PC10] (AB265585)
  • IP

Supplier Data

Immunoprecipitation - Anti-PCNA antibody [PC10] (AB265585)

PCNA was immunoprecipitated from 1 mg TCMK-1 cell lysate using ab265585 at 20 μl/mg lysate. Western blot was performed on the immunoprecipitate using ab265585 at 1/1000 dilution.

Lane 1 : ab265585 IP in TCMK-1 whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585)

Predicted band size: 29 kDa

true

Exposure time: 3min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PC10

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, Flow Cyt, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Pcna.

P04961

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p>Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/1 mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Confers DNA tethering and processivity to DNA polymerases and other proteins (PubMed : 24695737, PubMed : 24939902, PubMed : 35585232). Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).
See full target information PCNA
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Anatomy & cell biology 56:526-537 PubMed37654017

2023

Effect of Sofosbuvir on rats' ovaries and the possible protective role of vitamin E: biochemical and immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Neven A Ebrahim,Hussein Abdelaziz Abdalla,Neimat Abd Elhakam Yassin,Aya Elsayed Maghrabia,Amira Ibrahim Morsy

Journal of immunology research 2022:1917585 PubMed35692504

2022

Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Xiaolin Ji,Zhenguang Chen,Zhichao Yao

Experimental and therapeutic medicine 23:7 PubMed34815759

2021

IRF2-induced Claudin-7 suppresses cell proliferation, invasion and migration of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Weidong Yang
View all publications
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Product promise

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