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AB97909

Anti-PCNP antibody

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(3 Publications)

Rabbit Polyclonal PCNP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PCNP aa 1-50.

View Alternative Names

PEST proteolytic signal-containing nuclear protein, PCNP, PEST-containing nuclear protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNP antibody (AB97909)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNP antibody (AB97909)

ab97909 at 1/500 dilution staining PCNP in H1299 xenograft by Immunohistochemistry, Paraffin-embedded tissue.

Western blot - Anti-PCNP antibody (AB97909)
  • WB

Unknown

Western blot - Anti-PCNP antibody (AB97909)

12% SDS-PAGE.

All lanes:

Western blot - Anti-PCNP antibody (ab97909) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PCNP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8WW12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNP also called PEST-containing nuclear protein consists of around 178 amino acids and has a mass of approximately 21 kDa. PCNP localizes mainly in the nucleus of cells and shows expression in various tissues including the testis and skeletal muscle. The presence of PEST sequences suggests the target may experience rapid degradation which is typical for proteins that regulate essential cellular processes.
Biological function summary

This protein participates in cell cycle regulation and ubiquitination pathways suggesting its role in protein turnover within cells. PCNP often interacts with other proteins as part of larger complexes to influence proteolysis and cell cycle progression. The interaction with molecules containing ubiquitin-like domains indicates its involvement in tagging specific proteins for degradation helping maintain cellular homeostasis.

Pathways

PCNP connects strongly to the ubiquitin-proteasome pathway and the regulation of transcription. Its interactions with proteins such as RNF115 indicate its role in protein ubiquitination a fundamental process for maintaining cell integrity and function. Another relevant link is to cyclin-dependent kinases suggesting a contribution to cell cycle checkpoints and transitions essential for controlled cell division and proliferation.

PCNP's role highlights its potential involvement in oncogenesis and neurodegenerative disorders. Aberrant expression or regulation can connect PCNP to cancer progression by influencing the degradation of cell cycle regulators. Additionally defects in ubiquitination processes can tie this protein to conditions such as Alzheimer's disease where misfolded proteins accumulate. Interaction with proteins like beta-amyloid precursor protein suggests a possible link to neurodegenerative pathways impacting both disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in cell cycle regulation.
See full target information PCNP
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108370 PubMed38034348

2023

Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Wu Pan,Wen-Jin Chen,Da Xu,Wen-Bin Guan,Lin Li,Jia-Xin Chen,Wei-Jie Chen,Ke-Qin Dong,Jian-Qing Ye,Si-Shun Gan,Wang Zhou,Xin-Gang Cui

Cell death discovery 8:228 PubMed35468892

2022

LINC00858 promotes colon cancer progression through activation of STAT3/5 signaling by recruiting transcription factor RAD21 to upregulate PCNP.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xu,Kun Wu,Jin Shi,Lindong Ji,Xudong Song,Guoquan Tao,Shutao Zheng,Li Zhang,Baofei Jiang

Molecular cell 25:331-43 PubMed17289582

2007

Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Ciccia,Chen Ling,Rachel Coulthard,Zhijiang Yan,Yutong Xue,Amom Ruhikanta Meetei,El Houari Laghmani,Hans Joenje,Neil McDonald,Johan P de Winter,Weidong Wang,Stephen C West
View all publications
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Product promise

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