AB154261

Anti-PCOLCE antibody

Name: Anti-PCOLCE antibody (ab154261) | Abcam
Brand: Abcam Limited
SKU: AB154261
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal PCOLCE antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCOLCE aa 1 to C-terminus.

View Alternative Names

PCPE1, PCOLCE, Procollagen C-endopeptidase enhancer 1, Procollagen COOH-terminal proteinase enhancer 1, Type 1 procollagen C-proteinase enhancer protein, Type I procollagen COOH-terminal proteinase enhancer, PCPE-1, Procollagen C-proteinase enhancer 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCOLCE antibody (AB154261)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCOLCE antibody (AB154261)

Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling PCOLCE with ab154261 at 1/500 dilution.

Unknown

Western blot - Anti-PCOLCE antibody (AB154261)

10% SDS PAGE

All lanes:

Western blot - Anti-PCOLCE antibody (ab154261) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PCOLCE aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15113

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCOLCE known as Procollagen C-endopeptidase enhancer is a glycoprotein with an approximate mass of 55 kDa. It resides mainly in the extracellular matrix playing a role in collagen processing. PCOLCE enhances the activity of the enzyme procollagen C-proteinase which is critical for converting procollagen to collagen by cleaving its C-terminal propeptide. PCOLCE expression occurs in tissues such as skin tendons and cartilage where collagen is abundant.

Biological function summary

PCOLCE serves as an enhancer of enzyme activity important for proper collagen maturation. This protein strongly associates with the biosynthesis of collagen fibrils being part of the larger complex responsible for proper collagen assembly. The effective regulation of collagen fibrils is essential for maintaining structural integrity and strength of various tissues such as the skin and bone matrix.

Pathways

PCOLCE plays an important role in the collagen biosynthesis and modifying pathways. It collaborates closely with other proteins like BMP1 (Bone Morphogenetic Protein 1) which also participates in the procollagen processing activity. Through its precise regulation PCOLCE affects tissue repair and development pathways ensuring efficient collagen synthesis and deposition critical for functional extracellular matrix formation.

Mutations or dysregulation in PCOLCE can potentially relate to connective tissue disorders such as Ehlers-Danlos syndrome. In these conditions improper collagen processing occurs affecting tissue elasticity and strength. Studies suggest that abnormal PCOLCE activity may also influence fibrosis where excessive collagen deposition leads to tissue and organ dysfunction. In these contexts PCOLCE interaction with proteins like BMP1 and other collagen-modifying enzymes is significant affecting the progression of such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.

See full target information PCOLCE

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 51:102185 PubMed39522303

2024

Prognostic significance of combined PCPE-1 and α-fetoprotein for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhua Zhang,Wanqi Chen,Xuelan Peng,Zhiguang Zhang,Shangjiu Yang,Li Zhong

Asia-Pacific journal of clinical oncology 21:48-57 PubMed39403870

2024

Procollagen C-protease enhancer protein promotes glioma growth by activating ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenchao Ma,Daxing Huang,Lixin Ru,Ming Chen

PeerJ 10:e12493 PubMed35036081

2022

Key genes associated with prognosis and metastasis of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Zhong,Zeying Jiang,Xiangdong Wang,Honglei Wang,Meiyi Song,Wenfang Chen,Shicong Yang

Acta biochimica et biophysica Sinica 52:207-210 PubMed31942931

2020

Enhanced store-operated calcium entry in platelets is associated with acute coronary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Guo,Jiayu Zhang,Jianhua Zhu,Qing-Hui Chen,Renjun Wang,Le Gui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCOLCE antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCOLCE antibody (AB154261)

Western blot - Anti-PCOLCE antibody (AB154261)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise