AB243025

Anti-PCP4 antibody [CL5306]

Name: Anti-PCP4 antibody [CL5306] (ab243025) | Abcam
Brand: Abcam Limited
SKU: AB243025
Price: 630 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Mouse Monoclonal PCP4 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PCP4 aa 1 to C-terminus.

View Alternative Names

PEP19, PCP4, Calmodulin regulator protein PCP4, Brain-specific polypeptide PEP-19, Purkinje cell protein 4

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling PCP4 with ab243025 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Negative control - Formalin-fixed, paraffin-embedded human tonsil tissue stained for PCP4 with ab243025 at a 1 : 500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for PCP4 with ab243025 at a 1 : 500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling PCP4 with ab243025 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Formalin-fixed, paraffin-embedded rat cerebellum tissue stained for PCP4 with ab243025 at a 1 : 500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Formalin-fixed, paraffin-embedded mouse cerebellum tissue stained for PCP4 with ab243025 at a 1 : 500 dilution in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL5306

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PCP4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48539

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCP4 also known as Purkinje cell protein 4 functions as a calcium-binding protein predominantly involved in the regulation of calmodulin signaling. It weighs approximately 7 kDa and exhibits a high expression in the central nervous system especially in Purkinje cells of the cerebellum. PCP4 also shows expression in the hippocampus and other brain regions suggesting its role in neuronal activities and functions.

Biological function summary

PCP4 regulates calcium-dependent signaling pathways by interacting with calmodulin. It plays an important role in modulating neural signaling and plasticity impacting synaptic function and neuronal communication. PCP4 is not part of a large complex instead it works directly with calmodulin to influence calcium dynamics therefore affecting various neuronal processes.

Pathways

PCP4 participates actively in calcium-signaling pathways which impact neuronal excitability and synaptic transmission. It closely interacts through these pathways with calmodulin potentially affecting other downstream effectors like kinase enzymes. By modulating these pathways PCP4 influences neurotransmission and can alter neuronal circuit functions which is important for learning and memory processes.

PCP4 has a significant link with certain neurological conditions including schizophrenia and autism spectrum disorders. Changes in the expression or mutation of PCP4 impact neuronal signaling and excitability contributing to the pathology of these conditions. PCP4's interaction with calmodulin may also influence these diseases as altered calcium-signaling dynamics are often observed in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a modulator of calcium-binding by calmodulin. Thereby, regulates calmodulin activity and the different processes it controls (PubMed : 19106096, PubMed : 23204517, PubMed : 27876793). For instance, may play a role in neuronal differentiation through activation of calmodulin-dependent kinase signaling pathways (PubMed : 21491429).

See full target information PCP4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:277 PubMed39725889

2024

Investigating the inflammatory mechanism of notoginsenoside R1 in Diabetic nephropathy via ITGB8 based on network pharmacology and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

ChangYan Li,Chen Geng,JiangMing Wang,Luyao Shi,JingYuan Ma,Zhang Liang,WenXing Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCP4 antibody [CL5306]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCP4 antibody [CL5306] (AB243025)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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