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AB232721

Anti-PCPE-2 antibody

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(2 Publications)

Rabbit Polyclonal PCPE-2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCOLCE2 aa 250 to C-terminus.

View Alternative Names

PCPE2, UNQ250/PRO287, PCOLCE2, Procollagen C-endopeptidase enhancer 2, Procollagen COOH-terminal proteinase enhancer 2, PCPE-2, Procollagen C-proteinase enhancer 2

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)

Formalin-fixed, paraffin-embedded human liver tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)

Formalin-fixed, paraffin-embedded human stomach tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)

Formalin-fixed, paraffin-embedded human prostate tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (AB232721)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PCPE-2 antibody (AB232721)
  • WB

Supplier Data

Western blot - Anti-PCPE-2 antibody (AB232721)

All lanes:

Western blot - Anti-PCPE-2 antibody (ab232721) at 2 µg/mL

All lanes:

Mouse heart tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-PCPE-2 antibody (AB232721)
  • WB

Supplier Data

Western blot - Anti-PCPE-2 antibody (AB232721)

All lanes:

Western blot - Anti-PCPE-2 antibody (ab232721) at 2 µg/mL

All lanes:

Recombinant human PCPE-2 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PCOLCE2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UKZ9

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Primary Antibodies

AB34710

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Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCPE-2 also known as procollagen C-endopeptidase enhancer 2 enhances the activity of procollagen C-proteinase. It weighs approximately 42 kDa. PCPE-2 is expressed in various tissues including liver and kidney. This protein influences collagen biosynthesis by binding to substrates and increasing enzymatic activity.
Biological function summary

PCPE-2 assists in the maturation of type I II and III procollagens. It does not form a part of any larger complex but it increases the cleavage efficiency of the C-terminal propeptide from procollagens. By promoting collagen fibril formation PCPE-2 plays a role in tissue organization and structure.

Pathways

The role of PCPE-2 in collagen biosynthesis connects it to the extracellular matrix organization pathway. Additionally this protein interacts with enzymes such as bone morphogenetic protein 1 (BMP1) which are important for collagen maturation. These interactions facilitate the assembly and structure of the extracellular matrix.

PCPE-2's involvement in collagen maturation links it to fibrosis a condition characterized by excessive collagen deposition. It also relates to osteogenesis imperfecta a disorder affecting bone strength. In these conditions PCPE-2 works alongside proteins like BMP1 influencing collagen processing and accumulation thereby affecting tissue characteristics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.
See full target information PCOLCE2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14320 PubMed39158018

2024

Single-cell RNA sequencing reveals the CRTAC1 population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Tang,Dachun Zhuo,Yuexin Yu,Weilin Pu,Yanyun Ma,Yuting Zhang,Yan Huang,Qing Zhang,Kunhai Tang,Chen Meng,Di Yang,Lu Bai,Dongyi He,Li Jin,Hejian Zou,Huji Xu,Qi Zhu,Jiucun Wang,Yuanyuan Chen,Jing Liu

Molecular cancer 21:92 PubMed35366893

2022

Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Anqi Sun,Aihui Yan,Jian Yao,Haibo Huang,Ziming Gao,Tao Han,Jia Gu,Ni Li,Huizhe Wu,Kai Li
View all publications
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Product promise

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