Rabbit Polyclonal PCPE-2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PCOLCE2 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PCOLCE2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKZ9
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.
Alternative names
PCPE2, UNQ250/PRO287, PCOLCE2, Procollagen C-endopeptidase enhancer 2, Procollagen COOH-terminal proteinase enhancer 2, PCPE-2, Procollagen C-proteinase enhancer 2
Recommended products
Rabbit Polyclonal PCPE-2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PCOLCE2 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PCOLCE2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKZ9
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCPE-2 also known as procollagen C-endopeptidase enhancer 2 enhances the activity of procollagen C-proteinase. It weighs approximately 42 kDa. PCPE-2 is expressed in various tissues including liver and kidney. This protein influences collagen biosynthesis by binding to substrates and increasing enzymatic activity.
- Biological function summary
PCPE-2 assists in the maturation of type I II and III procollagens. It does not form a part of any larger complex but it increases the cleavage efficiency of the C-terminal propeptide from procollagens. By promoting collagen fibril formation PCPE-2 plays a role in tissue organization and structure.
- Pathways
The role of PCPE-2 in collagen biosynthesis connects it to the extracellular matrix organization pathway. Additionally this protein interacts with enzymes such as bone morphogenetic protein 1 (BMP1) which are important for collagen maturation. These interactions facilitate the assembly and structure of the extracellular matrix.
- Associated diseases and disorders
PCPE-2's involvement in collagen maturation links it to fibrosis a condition characterized by excessive collagen deposition. It also relates to osteogenesis imperfecta a disorder affecting bone strength. In these conditions PCPE-2 works alongside proteins like BMP1 influencing collagen processing and accumulation thereby affecting tissue characteristics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
Western blot - Anti-PCPE-2 antibody (ab232721)
All lanes: Western blot - Anti-PCPE-2 antibody (ab232721) at 2 µg/mL
All lanes: Recombinant human PCPE-2 protein
SecondaryAll lanes: HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Western blot - Anti-PCPE-2 antibody (ab232721)
All lanes: Western blot - Anti-PCPE-2 antibody (ab232721) at 2 µg/mL
All lanes: Mouse heart tissue lysate
SecondaryAll lanes: HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (ab232721)
Formalin-fixed, paraffin-embedded human liver tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (ab232721)
Formalin-fixed, paraffin-embedded human stomach tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (ab232721)
Formalin-fixed, paraffin-embedded human kidney tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (ab232721)
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (ab232721)
Formalin-fixed, paraffin-embedded human prostate tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCPE-2 antibody (ab232721)
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for PCPE-2 using ab232721 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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