Rabbit Polyclonal PCSK2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Pcsk2 aa 600 to C-terminus.
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- eLife 9:2020SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHugo Ducuing et. al.PubMed 33345773
- eLife 9:2020Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex J B Kreutzberger,Volker Kiessling,Catherine A Doyle,Noah Schenk,Clint M Upchurch,Margaret Elmer-Dixon,Amanda E Ward,Julia Preobraschenski,Syed S Hussein,Weronika Tomaka,Patrick Seelheim,Iman Kattan,Megan Harris,Binyong Liang,Anne K Kenworthy,Bimal N Desai,Norbert Leitinger,Arun Anantharam,J David Castle,Lukas K TammPubMed 33164744
- Journal of cellular physiology 235:6875-68862020Goldfish adipocytes are pancreatic beta cell-like, glucose-responsive insulin-producing cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyelén Melisa Blanco,Juan Ignacio Bertucci,Suraj UnniappanPubMed 31989646
- Endocrine connections 8:8-192018Apatinib inhibits tumor growth and angiogenesis in PNET models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Wu,Jianjun Zhou,Jing Guo,Zhan Hua,Jianchen Li,Zai WangPubMed 30557852
- eLife 5:2016HID-1 is required for homotypic fusion of immature secretory granules during maturation.
- Regulatory peptides 152:54-602008Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey Gagnon,Janice Mayne,Majambu Mbikay,John Woulfe,Michel ChrétienPubMed 18706454
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Pcsk2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P21661
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
Target data
Function
Serine endopeptidase which is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells.
Alternative names
NEC2, PCSK2, Neuroendocrine convertase 2, NEC 2, KEX2-like endoprotease 2, Prohormone convertase 2, Proprotein convertase 2, PC2
Recommended products
Rabbit Polyclonal PCSK2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Pcsk2 aa 600 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Pcsk2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P21661
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PCSK2 also known as proprotein convertase 2 is an enzyme with a molecular mass of approximately 75 kDa. It functions by processing various precursor proteins into their active forms. PCSK2 belongs to the family of serine proteases and is mainly expressed in endocrine and neuroendocrine tissues including the pituitary gland kidneys and pancreas. This protein is important in the maturation of peptide hormones and neuropeptides impacting physiological functions.
- Biological function summary
PCSK2 is responsible for activating various prohormones into their active hormone counterparts. It accomplishes this by cleaving specific sites on precursor proteins. PCSK2 does not operate as part of a complex but independently participates in proteolytic processing essential for proper hormone functioning. This enzyme's activity is critical for the synthesis of key hormones like insulin and glucagon which regulate glucose metabolism.
- Pathways
PCSK2 is a significant part of hormone processing pathways. Specifically it contributes to the insulin-processing pathway which is important for energy regulation and homeostasis. PCSK2 works closely with related proteins such as PCSK1 (proprotein convertase 1) in the cleavage of precursor forms of various hormones. The coordinated action of these enzymes ensures the production of bioactive hormones required for normal metabolic functions.
- Associated diseases and disorders
PCSK2 is associated with conditions like diabetes and obesity due to its role in processing hormones that regulate metabolism. Dysregulation or mutations in the PCSK2 gene can disrupt insulin and glucagon maturation leading to imbalances in blood sugar levels. Additionally variations in PCSK2 may influence fat storage and energy expenditure contributing to obesity. The interaction between PCSK2 and insulin-related pathways highlights its importance in maintaining metabolic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-PCSK2 antibody (ab3533)
All lanes: Western blot - Anti-PCSK2 antibody (ab3533) at 1/500 dilution
All lanes: Mouse cerebellum cell lysate at 25 µg
Predicted band size: 71 kDa
Observed band size: 65 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)
Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)
Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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