Anti-PCSK2 antibody [EPR23578-19]

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1 : 2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1 : 10000 (0.053 μg/ml) [Panel C], and anti-Neuropeptide Y stained on chromaffin cells (ab221145; red; Opal™570) at 1 : 2000 (0.279 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab274418, ab272494, and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1 : 2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1 : 10000 (0.053 μg/ml) [Panel C], and anti-Collagen VI stained on extracellular matrix (ab182744; red; Opal™570) at 1 : 1000 (1.518 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab274418, ab272494, and ab182744 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TT cells labelling PCSK2 with ab274418 at 1/100 (5.25 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in TT cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on myenteric neurons of human colon (PMID : 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized TT (Human thyroid carcinoma epithelial cell) cells labelling PCSK2 with ab274418 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human adrenal gland. The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BON^D® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human pancreatic islets (PMID : 8916141, PMID : 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^. RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; red; Opal™690) at 1 : 2000 (0.263 μg/ml) [Panel B], anti-C11B2/CYP11B2 stained on zona glomerulosa (ab249476; cyan; Opal™520) at 1 : 500 (4.478 μg/ml) [Panel C], and anti-SULT2A1 stained on zona reticularis (ab240333; yellow; Opal™570) at 1 : 500 (4.504 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab274418, ab249476, and ab240333 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• WB

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Western blot - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

55-kDa PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 8557169, 14693708).
This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution

All lanes:

Human brain tissue lysate at 40 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 71 kDa

Observed band size: 55 kDa

false

• WB

Unknown

Western blot - Anti-PCSK2 antibody [EPR23578-19] (AB274418)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 37 seconds.

70-kDa pro-PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 8557169, 14693708).

All lanes:

Western blot - Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution

All lanes:

TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 71 kDa

Observed band size: 70 kDa

false