JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB280997

Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PCSK2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

NEC2, PCSK2, Neuroendocrine convertase 2, NEC 2, KEX2-like endoprotease 2, Prohormone convertase 2, Proprotein convertase 2, PC2

1 Images
Sandwich ELISA - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (Detector) (AB280997)
  • sELISA

Lab

Sandwich ELISA - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (Detector) (AB280997)

Sandwich ELISA of ab253587 with the capture antibody ab281147 dilution at 2 µg/mL and detector antibody ab280997 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23578-19

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB220363

Anti-PC1/3 antibody [EPR21908]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB275377

Anti-ALIX antibody [EPR23653-32]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ALIX antibody [EPR23653-32] (AB275377)

  • 5
  • 2
Primary Antibodies

AB254556

Anti-Galanin antibody [EPR22496-308]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab280997 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PCSK2. The recommended pair for sandwich ELISA is:
Capture: ab281147, Human PCSK2 Capture Antibody (unconjugated)
Detector: ab280997, Human PCSK2 Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PCSK2 also known as proprotein convertase 2 is an enzyme with a molecular mass of approximately 75 kDa. It functions by processing various precursor proteins into their active forms. PCSK2 belongs to the family of serine proteases and is mainly expressed in endocrine and neuroendocrine tissues including the pituitary gland kidneys and pancreas. This protein is important in the maturation of peptide hormones and neuropeptides impacting physiological functions.
Biological function summary

PCSK2 is responsible for activating various prohormones into their active hormone counterparts. It accomplishes this by cleaving specific sites on precursor proteins. PCSK2 does not operate as part of a complex but independently participates in proteolytic processing essential for proper hormone functioning. This enzyme's activity is critical for the synthesis of key hormones like insulin and glucagon which regulate glucose metabolism.

Pathways

PCSK2 is a significant part of hormone processing pathways. Specifically it contributes to the insulin-processing pathway which is important for energy regulation and homeostasis. PCSK2 works closely with related proteins such as PCSK1 (proprotein convertase 1) in the cleavage of precursor forms of various hormones. The coordinated action of these enzymes ensures the production of bioactive hormones required for normal metabolic functions.

PCSK2 is associated with conditions like diabetes and obesity due to its role in processing hormones that regulate metabolism. Dysregulation or mutations in the PCSK2 gene can disrupt insulin and glucagon maturation leading to imbalances in blood sugar levels. Additionally variations in PCSK2 may influence fat storage and energy expenditure contributing to obesity. The interaction between PCSK2 and insulin-related pathways highlights its importance in maintaining metabolic health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Serine endopeptidase which is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells.
See full target information PCSK2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com