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AB28770

Anti-PCSK9 antibody

4

(2 Reviews)

|

(17 Publications)

Goat Polyclonal PCSK9 antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human PCSK9 aa 650 to C-terminus.

View Alternative Names

NARC1, PSEC0052, PCSK9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PCSK9 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NBP7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab28770 was purified from goat serum by ammonium sulphate precipitation prior to immunogen affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
Biological function summary

PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.

Pathways

PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.

PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed : 18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed : 17461796, PubMed : 18197702, PubMed : 18799458, PubMed : 22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed : 18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information PCSK9
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40563628

2025

Inhibition of PCSK9 Attenuates Liver Endothelial Cell Activation Induced by Colorectal Cancer Stem Cells During Liver Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ander Martin,Daniela Gerovska,Marcos J Arauzo-Bravo,Maitane Duarte García-Escudero,Helena García García,Iratxe Bañares,Naroa Fontal,Geraldine Siegfried,Serge Evrad,Simon Pernot,Abdel-Majid Khatib,Iker Badiola

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2408514 PubMed40192514

2025

PCSK9 Promotes the Malignancy of Triple-negative Breast Cancer Cells by Reducing Cholesterol Levels at the Plasma Membrane to Activate EGFR and HER3.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhong Li,Renfei Wu,Kathy Qian Luo

Theranostics 13:531-542 PubMed36632236

2023

SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nunzia D'Onofrio,Francesco Prattichizzo,Raffaele Marfella,Celestino Sardu,Elisa Martino,Lucia Scisciola,Lorenza Marfella,Rosalba La Grotta,Chiara Frigé,Giuseppe Paolisso,Antonio Ceriello,Maria Luisa Balestrieri

Journal of experimental & clinical cancer research : CR 41:303 PubMed36242053

2022

PCSK9 promotes the progression and metastasis of colon cancer cells through regulation of EMT and PI3K/AKT signaling in tumor cells and phenotypic polarization of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Shuangshuang Li,Huanhua Luo,Qi Lu,Shuwen Yu

Foods (Basel, Switzerland) 10: PubMed34829135

2021

The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Jimin Hong,Minji Kim,Bohkyung Kim

Atherosclerosis 326:47-55 PubMed33933263

2021

Effects of proprotein convertase subtilisin kexin type 9 modulation in human pancreatic beta cells function.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Ramin-Mangata,Aurélie Thedrez,Brice Nativel,Nicolas Diotel,Valentin Blanchard,Matthieu Wargny,Audrey Aguesse,Stéphanie Billon-Crossouard,Cécile Vindis,Cédric Le May,Philippe Hulin,Mathieu Armanet,Valery Gmyr,François Pattou,Mikaël Croyal,Olivier Meilhac,Estelle Nobécourt,Bertrand Cariou,Gilles Lambert

Frontiers in oncology 10:609663 PubMed33489919

2021

Proprotein Convertase Subtilisin/Kexin Type 9 Promotes Gastric Cancer Metastasis and Suppresses Apoptosis by Facilitating MAPK Signaling Pathway Through HSP70 Up-Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Beili Xu,Shuyu Li,Yong Fang,Yanting Zou,Dongqiang Song,Shuncai Zhang,Yu Cai

JCI insight 5: PubMed33119548

2020

microRNA-483 ameliorates hypercholesterolemia by inhibiting PCSK9 production.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjie Dong,Ming He,Jie Li,Ariane Pessentheiner,Chen Wang,Jin Zhang,Yameng Sun,Wei-Ting Wang,Yuqing Zhang,Junhui Liu,Shen-Chih Wang,Po-Hsun Huang,Philip Lsm Gordts,Zu-Yi Yuan,Sotirios Tsimikas,John Yj Shyy

PloS one 14:e0220254 PubMed31437157

2019

Proprotein convertase subtilisin/kexin type 9 (PCSK9) in Alzheimer's disease: A genetic and proteomic multi-cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Picard,Alexandre Poirier,Stéphanie Bélanger,Anne Labonté,Daniel Auld,Judes Poirier

BMC biology 17:4 PubMed30646909

2019

In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Carreras,Luna Simona Pane,Roberto Nitsch,Katja Madeyski-Bengtson,Michelle Porritt,Pinar Akcakaya,Amir Taheri-Ghahfarokhi,Elke Ericson,Mikael Bjursell,Marta Perez-Alcazar,Frank Seeliger,Magnus Althage,Ralph Knöll,Ryan Hicks,Lorenz M Mayr,Rosie Perkins,Daniel Lindén,Jan Borén,Mohammad Bohlooly-Y,Marcello Maresca
View all publications
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Product promise

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