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Rabbit Recombinant Monoclonal PCTAIRE1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail
  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail
  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail
  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Expected
Expected
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
1/20
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).

Alternative names

PCTAIRE1, PCTK1, CDK16, Cyclin-dependent kinase 16, Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1

Recommended products

Rabbit Recombinant Monoclonal PCTAIRE1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13210
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PCTAIRE1 also known as Cyclin-dependent kinase 16 (CDK16) plays a significant mechanical role as a serine/threonine kinase. This protein with a molecular weight of approximately 45 kDa participates in various intracellular processes. It shows expression in multiple tissues including prominently in the brain and testis. PCTAIRE1 regulates cellular events by phosphorylating target proteins thereby influencing their function.

Biological function summary

PCTAIRE1 influences certain cellular functions such as signal transduction and neuron differentiation. It exists as part of complexes that include cyclins which modulate its kinase activity. In neurons PCTAIRE1 contributes to processes like neurite outgrowth and synaptic function. Research indicates a role in the transport of synaptic vesicles hinting at its involvement in neurotransmitter release and communication between neurons.

Pathways

PCTAIRE1 serves as a component of pathways such as the Wnt signaling pathway and the cell cycle. Its function aligns with several other proteins including CDK16 in modulating processes like cell proliferation and neural development. PCTAIRE1 interacts closely with proteins like Cyclin Y which help guide its role in these pathways ensuring proper progression of related biological events.

Associated diseases and disorders

PCTAIRE1 has associations with neurological disorders and certain cancers. Abnormal expression or activity of PCTAIRE1 may relate to conditions like schizophrenia or glioblastoma. The protein's interaction with Cyclin Y is key in the context of cancer where dysregulation of these interactions can contribute to tumor progression. Alterations in PCTAIRE1 function and its pathways highlight its potential as a target for therapeutic strategies in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail

    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: PCTAIRE1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HEK293 cell lysate (20 μg)
    Lane 4: HepG2 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181208 observed at 63 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab181208 was shown to recognize PCTAIRE1 when PCTAIRE1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1 knockout samples were subjected to SDS-PAGE. ab181208 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    Predicted band size: 56 kDa

  • Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    Intracellular flow cytometric analysis of BxPC-3cells (2% paraformaldehyde-fixed) labeling PCTAIRE1with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail

    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

    All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

    Lane 1: Human testis cell lysate at 10 µg

    Lane 2: Human fetal heart cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail

    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

    All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

    All lanes: BxPC-3 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail

    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    PCTAIRE1 Western blot staining using rabbit Anti-PCTAIRE1 antibody

    Western blot: Rabbit Monoclonal [EPR13210] to PCTAIRE1 ab181208 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

    A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in CDK16 knockout A549 cell line.

    To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

    Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

    Lane 1: Wild-type A549 at 20 µg

    Lane 2: CDK16 knockout A549 at 20 µg

    Lane 3: HT-1080 at 20 µg

    Lane 4: HL-60 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 55 kDa

    Observed band size: 55 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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