Rabbit Recombinant Monoclonal PCTAIRE1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--western-blot-img100434.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)")
![Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--flow-cytometry-intracellular-img70453.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)")
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--western-blot-img57362.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)")
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--western-blot-img81885.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Alternative names
Cyclin-dependent kinase 16, Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1, PCTK1, PCTAIRE1, CDK16
Recommended products
Rabbit Recombinant Monoclonal PCTAIRE1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR13210
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--western-blot-img100434.jpg "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)")
Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: PCTAIRE1 knockout HAP1 cell lysate (20 μg)
Lane 3: HEK293 cell lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181208 observed at 63 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab181208 was shown to recognize PCTAIRE1 when PCTAIRE1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1 knockout samples were subjected to SDS-PAGE. ab181208 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
Predicted band size: 56 kDa
![Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--flow-cytometry-intracellular-img70453.jpg "Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)")
Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
Intracellular flow cytometric analysis of BxPC-3cells (2% paraformaldehyde-fixed) labeling PCTAIRE1with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--western-blot-img57362.jpg "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)")
Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.
All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution
Lane 1: Human testis cell lysate at 10 µg
Lane 2: Human fetal heart cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Predicted band size: 56 kDa
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208), expandable thumbnail](https://content.abcam.com/products/images/pctaire1-antibody-epr13210-ab181208--western-blot-img81885.jpg "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)")
Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.
All lanes: Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution
All lanes: BxPC-3 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Predicted band size: 56 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com