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AB184944

Anti-PCTP antibody [EPR15390]

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(2 Publications)

Rabbit Recombinant Monoclonal PCTP antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

STARD2, PCTP, Phosphatidylcholine transfer protein, PC-TP, START domain-containing protein 2, StAR-related lipid transfer protein 2, StARD2

3 Images
Western blot - Anti-PCTP antibody [EPR15390] (AB184944)
  • WB

Supplier Data

Western blot - Anti-PCTP antibody [EPR15390] (AB184944)

All lanes:

Western blot - Anti-PCTP antibody [EPR15390] (ab184944) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

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Flow Cytometry (Intracellular) - Anti-PCTP antibody [EPR15390] (AB184944)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PCTP antibody [EPR15390] (AB184944)

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed K562 cells labeling PCTP with ab184944 at a 1/200 dilution (Red), with a negative control rabbit IgG (Green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.

Immunoprecipitation - Anti-PCTP antibody [EPR15390] (AB184944)
  • IP

Supplier Data

Immunoprecipitation - Anti-PCTP antibody [EPR15390] (AB184944)

Western blot analysis on immunoprecipitation from Human fetal liver lysate (Lane 1), labeling PCTP using ab184944 at 1/50 dilution Lane 2 shows PBS negative control. Secondary antibody, HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PCTP antibody [EPR15390] (ab184944)

Predicted band size: 25 kDa

Observed band size: 25 kDa

true

  • Carrier free

    Anti-PCTP antibody [EPR15390] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15390

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCTP also known as Phosphatidylcholine Transfer Protein is a lipid-binding protein important for lipid metabolism. It weighs approximately 26 kDa and is expressed in many tissues including liver adipose tissue and testis. Mechanically PCTP binds to phosphatidylcholine and transfers it between membranes. This transfer is essential in maintaining the lipid composition and function of cell membranes. The target enables effective lipid signaling in various cellular environments.
Biological function summary

PCTP plays a role in the management of lipid homeostasis and membrane structure. The protein does not form part of a larger complex but interacts with various membrane-associated lipid molecules. Its activity affects the distribution of lipids within cells and contributes to processes such as membrane trafficking and signal transduction. This activity supports cellular functions like proliferation differentiation and apoptosis through regulated lipid signaling.

Pathways

PCTP participates in lipid signaling and transport mechanisms. The protein associates with key pathways like glycerophospholipid metabolism and glycerolipid metabolism influencing lipid distribution and cellular communication. It interacts with proteins such as ABCA1 and LXR which play significant roles in cholesterol and phospholipid transport. These interactions help regulate lipid levels in the cell ensuring proper biological function within these pathways.

PCTP links to conditions like cardiovascular disease and metabolic syndrome. Aberrant PCTP activity can influence lipid levels and contribute to the pathogenesis of these disorders. The protein interacts with molecules like oxidized LDL in the context of cardiovascular disease impacting lipid accumulation and atherosclerosis. Understanding PCTP's role in these conditions may offer insights into therapeutic strategies targeting lipid metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of phosphatidylcholine between membranes. Binds a single lipid molecule.
See full target information PCTP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Reproductive sciences (Thousand Oaks, Calif.) 27:1758-1769 PubMed32072607

2020

Changes in Placental Nutrient Transporter Protein Expression and Activity Across Gestation in Normal and Obese Women.

Applications

Unspecified application

Species

Unspecified reactive species

Laura B James-Allan,Stephanie Teal,Theresa L Powell,Thomas Jansson

Journal of hepatology 63:1077-85 PubMed26144659

2015

Expression and functionality of Toll- and RIG-like receptors in HepaRG cells.

Applications

Unspecified application

Species

Unspecified reactive species

Souphalone Luangsay,Malika Ait-Goughoulte,Maud Michelet,Océane Floriot,Marc Bonnin,Marion Gruffaz,Michel Rivoire,Simon Fletcher,Hassan Javanbakht,Julie Lucifora,Fabien Zoulim,David Durantel
View all publications
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Product promise

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