AB248075

Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PCYOX1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0908, PCL1, UNQ597/PRO1183, PCYOX1, Prenylcysteine oxidase 1, Prenylcysteine lyase

3 Images

Unknown

Western blot - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

This data was developed using ab124966, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (ab248075) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysates at 10 µg

Lane 2:

HepG2 cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Lane 4:

HeLa cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

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Lab

Western blot - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

Blocking and dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124966).

All lanes:

Western blot - Anti-PCYOX1 antibody [EPR5611] (<a href='/en-us/products/primary-antibodies/pcyox1-antibody-epr5611-ab124966'>ab124966</a>) at 1/10000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) cytoplasm lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) membrane lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) nuclear lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

false

Exposure time: 3s

OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-PCYOX1 antibody [EPR5611]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5611

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248075 is the carrier-free version of ab124966.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCYOX1 also known as prenylcysteine oxidase 1 functions as an oxidase enzyme that specifically processes prenylated proteins by cleaving the thioether bond of prenylcysteines. This protein has a molecular weight of approximately 64 kDa. Expression of PCYOX1 is noted across various tissue types with marked presence in liver and kidney. Its localization suggests a possible involvement in cellular metabolism associated with lipid-modified proteins.

Biological function summary

The PCYOX1 protein enables degradation of prenylcysteine derivatives facilitating cellular processes related to lipid anchoring of signaling molecules. It is part of a larger complex responsible for prenylated protein turnover contributing to cellular regulation by modulating levels of signal transduction proteins anchored to membranes through prenylation.

Pathways

PCYOX1 participates in the isoprenoid metabolic pathway and is intricately linked to the post-translational modification pathway. This positions it alongside other prenylation-dependent proteins such as farnesylated and geranylgeranylated proteins which rely on similar modifications to localize to their functional membrane-bound states.

Alterations in PCYOX1 activity have connections to conditions like cancer and cardiovascular diseases. Dysregulation affects protein prenylation levels impacting pathways tied to oncogenes and proteins like Ras which is often implicated in malignancies. Likewise abnormal lipid metabolism associated with PCYOX1 links to cholesterol-related cardiovascular diseases influenced by protein interactions within these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine (PubMed : 10585463, PubMed : 11078725, PubMed : 12186880). Only active against free prenylcysteines and not prenylcysteine residues within prenylated proteins or peptides (By similarity). Involved in the final step in the degradation of prenylated proteins, by degrading prenylcysteines after the protein has been degraded (PubMed : 10585463).

See full target information PCYOX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free

Western blot - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

Western blot - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free (AB248075)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise