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Rabbit Recombinant Monoclonal PD-L1 antibody. Carrier free. Suitable for mIHC, IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Transfected cell line samples.

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PFlow CytWBICC/IF
Human
Tested
Tested
Tested
Tested
Expected
Transfected cell line
Not recommended
Tested
Not recommended
Not recommended
Tested

Images

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species
Transfected cell line
Dilution info
-
Notes

For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab228415 works better than ab205921 in western blot testing.

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

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Rabbit Recombinant Monoclonal PD-L1 antibody. Carrier free. Suitable for mIHC, IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Transfected cell line samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

28-8

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab228413 is the carrier-free version of abab205921.

Additional information on positive controls:

Tissue:
Tonsil- with hyperreactive changes
Note: Tonsil Specimens- is recommended to screen several hyper-reactive tonsils to find those with highest expression of PD-L1 in crypt epithelium, macrophages homing the germinal centers and interfollicular mononuclear leukocytes.
Tumor tissues- prescreened for positive tumor and inflammatory infiltrates
Note: Tumor Specimens- PD-L1 expression varies by tumor type so screening is recommended to find positive and negative tumor controls. Refer to web link publication below to find some suggested tumor types. Many tumor specimens have some inflammatory macrophages and mononuclear leukocytes. Look for specimens with high numbers of these cells
Cell Lines: Positives: B-CPAP- high, ES-2- medium, HCC70 - low

For IHC on FFPE tissues, antigen retrieval buffer (ab208572) and IHC detection kit HRP/DAB (ab209101) is recommended.

For primary negative control, isotype control, RabMAb negative control antibody (ab172730) is recommended.

For negative control sample, use cell line COLO205, see ab95363.
For PD-L1 protein, see ab167713

Recommended protocols:
For IHC usage on FFPE tissues, the following antigen solution is recommended with clone 28-8 - Universal HIER antigen retrieval reagent (ab208572)

Western blot usage
For clone 28-8, it is recommended to use Odyssey system. This system has the advantages of a wider dynamic range and less background than chemiluminescence.

Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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