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AB243877

Anti-PD-L1 antibody [BLR020E]

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(4 Publications)

Rabbit Recombinant Monoclonal PD-L1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CD274 aa 250 to C-terminus.

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A DyLight®-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Sup-M2 cells labelling PD-L1 with ab243877 and secondary HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD-L1 with ab243877 and secondary HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PD-L1 with ab243877 and HRP-conjugated goat anti-rabbit secondary. CD8 alpha is in red, and CD3E is in green. Counterstain is DAPI (blue)

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [BLR020E] (AB243877)

Formaldehyde-fixed HDML-2 cells labeling PDL-1 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (AB243877)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. Counterstain was DAPI.

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IP

Supplier Data

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (AB243877)

PD-L1 was immunoprecipitated from HeLa RKO cell lysate (1.0 mg per IP reaction, 20% loaded) with ab243877 at 20 μl/mg lysate. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution.

Lane 1 : rabbit anti-PD-L1 recombinant monoclonal antibody IP in RKO whole cell lysate.

Lane 2 : ab243877 IP in RKO whole cell lysate.

Lane 3 : ab243877 IP in RKO whole cell lysate.

Lane 4 : Control IgG in RKO whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (ab243877)

Predicted band size: 33 kDa

false

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • IP

Unknown

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (AB243877)

PDL-1 was immunoprecipitated from SUP-M2 whole cell lysate with ab243877. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution. Lane 1 : ab243877 IP in SUP-M2 whole cell lysate.

Lane 2 : Contol IgG in SUP-M2 whole cell lysate.

Detection : Chemiluminescence.

All lanes:

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (ab243877)

Predicted band size: 33 kDa

false

Western blot - Anti-PD-L1 antibody [BLR020E] (AB243877)
  • WB

Unknown

Western blot - Anti-PD-L1 antibody [BLR020E] (AB243877)

Detection : chemiluminescence.

All lanes:

Western blot - Anti-PD-L1 antibody [BLR020E] (ab243877) at 1/1000 dilution

Lane 1:

SUP-M2 whole cell lysate at 50 µg

Lane 2:

HDLM-2 whole cell lysate at 50 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

Ramos whole cell lysate at 50 µg

Lane 5:

Jurkat whole cell lysate at 50 µg

Lane 6:

PD-L2 whole cell lysate at 50 µg

Lane 7:

PD-L1 whole cell lysate at 50 µg

Lane 8:

HEK-293 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG antibody

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR020E

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF, IP

applications

Immunogen

Synthetic Peptide within Human CD274 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZQ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113046 PubMed40717771

2025

Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon gene-transcription by SWI/SNF-NuRD.

Applications

Unspecified application

Species

Unspecified reactive species

Gintare Lasaviciute,Kanwal Tariq,Anaswara Sugathan,Jaclyn Quin,Mareike Polenkowski,Ioana Bujila,Oleksii Skorokhod,Marita Troye-Blomberg,Eva Sverremark-Ekström,Ann-Kristin Östlund Farrants

Annals of gastroenterological surgery 8:507-520 PubMed38707229

2024

Exosomal circ_0032704 confers sorafenib resistance to hepatocellular carcinoma and contributes to cancer malignant progression by modulating the miR-514a-3p/PD-L1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyun Dou,Hongbo Zhu,Xia Xie,Cuiqin Huang,Hui Tan,Chuangjie Cao

International journal of oncology 62: PubMed36896779

2023

Bispecific GPC3/PD‑1 CAR‑T cells for the treatment of HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Li,Jie Qin,Tao Zhou,Yaqin Li,Xianyi Cheng,Zaizhong Chen,Junhui Chen,Wei V Zheng

Journal of the Egyptian National Cancer Institute 34:23 PubMed35644823

2022

The prognostic role of PD-1, PD-L1, ALK, and ROS1 proteins expression in non-small cell lung carcinoma patients from Egypt.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer A Bahnassy,Hoda Ismail,Marwa Mohanad,Ahmed El-Bastawisy,Hend F Yousef
View all publications
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Product promise

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