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Anti-PD-L1 antibody [CAL10] (ab237726) is a rabbit monoclonal antibody that is used to detect PD-L1 in Western Blot, IP, IHC-P, ICC/IF, ELISA, mIHC. Suitable for Human, samples.



- Specificity confirmed with PD-L1 knockout cell line validation

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Images

Western blot - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Western blot - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PIPELISAWBmIHCICC/IF
Human
Not recommended
Tested
Tested
Expected
Tested
Tested
Expected
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Transfected cell line - Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

Anti-PD-L1 antibody [73-10] ab228415 works better than this product in western blot testing.

Species
Human
Dilution info
1/1000
Notes

Anti-PD-L1 antibody [73-10] ab228415 works better than this product in western blot testing.

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

3 products for Alternative Version

Target data

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813410, PubMed:28813417, PubMed:31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813410, PubMed:28813417, PubMed:36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed:32929201). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813410, PubMed:28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Alternative names

Recommended products

Anti-PD-L1 antibody [CAL10] (ab237726) is a rabbit monoclonal antibody that is used to detect PD-L1 in Western Blot, IP, IHC-P, ICC/IF, ELISA, mIHC. Suitable for Human, samples.



- Specificity confirmed with PD-L1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
CAL10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

22 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com