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Anti-PD-L1 antibody [CAL10] (ab237726) is a rabbit monoclonal antibody that is used to detect PD-L1 in Western Blot, IP, IHC-P, ICC/IF, ELISA, mIHC. Suitable for Human, samples.



- Specificity confirmed with PD-L1 knockout cell line validation

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Images

Western blot - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Western blot - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-PD-L1 antibody [CAL10] (AB237726), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PIPELISAWBmIHCICC/IF
Human
Not recommended
Tested
Tested
Expected
Tested
Tested
Expected
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Transfected cell line - Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

Anti-PD-L1 antibody [73-10] ab228415 works better than this product in western blot testing.

Species
Human
Dilution info
1/1000
Notes

Anti-PD-L1 antibody [73-10] ab228415 works better than this product in western blot testing.

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

3 products for Alternative Version

Target data

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813410, PubMed:28813417, PubMed:31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813410, PubMed:28813417, PubMed:36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed:32929201). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813410, PubMed:28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Alternative names

Recommended products

Anti-PD-L1 antibody [CAL10] (ab237726) is a rabbit monoclonal antibody that is used to detect PD-L1 in Western Blot, IP, IHC-P, ICC/IF, ELISA, mIHC. Suitable for Human, samples.



- Specificity confirmed with PD-L1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
CAL10
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Purity >99%

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-PD-L1 antibody [CAL10] (ab237726) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Multiplex IHC (mIHC) in Human, samples.

What is the molecular weight of PD-L1?


Anti-PD-L1 [CAL10] (ab237726) specifically detects a band for PD-L1 (UniProt: Q9NZQ7) at a molecular weight of 33kDa.

Trusted by the scientific community


Anti-PD-L1 [CAL10] (ab237726) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-PD-L1 antibody [CAL10] (ab237726) has been confirmed by Western blot testing in CD274 Knockout A549 cell line, Human CD274 (PD-L1) knockout A549 cell line ab267054.



Other related products


We have a range of other formats of antibody clone [CAL10] also available for your convenience:
ab237726, Carrier free - Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-PD-L1 antibody [CAL10] ab267565, Anti-PD-L1 antibody [CAL10] - Mouse IgG1 (Chimeric) ab279292, Anti-PD-L1 antibody [CAL10] - Mouse IgG2a (Chimeric) ab279293, Anti-PD-L1 antibody [CAL10] - Rat IgG2a (Chimeric) ab279294, Carrier free - Anti-PD-L1 antibody [CAL10] - Mouse IgG1 (Chimeric) - BSA and Azide free ab279304, Carrier free - Anti-PD-L1 antibody [CAL10] - Mouse IgG2a (Chimeric) - BSA and Azide free ab279305, Carrier free - Anti-PD-L1 antibody [CAL10] - Rat IgG2a (Chimeric) - BSA and Azide free ab279306, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-PD-L1 antibody [CAL10] ab307643



Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.

Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

Associated diseases and disorders

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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