AB213524

Anti-PD-L1 antibody [EPR19759]

Name: Anti-PD-L1 antibody [EPR19759], recombinant (ab213524) | Abcam
Brand: Abcam Limited
SKU: AB213524
Price: 340 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
20ul selling size
What is this?

(1 Review)

(159 Publications)

Anti-PD-L1 antibody [EPR19759] (ab213524) is a rabbit monoclonal antibody detecting PD-L1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 90 publications

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

18 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling PD-L1 with ab213524 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Membrane staining on the human stomach cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PD-L1 with ab213524 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Membrane staining on the human placenta is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Tissue Microarrays stained for "Anti-PD-L1 antibody [EPR19759]" using "ab213524"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were perform heat mediated antigen retrieval before commencing with IHC staining protocol. The sections were incubated with ab213524 at +4°C overnight. The secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemical analysis of formalin fixed paraffin embedded Human tonsil labelling PD L1 with ab213524 at a concentration of 0.5 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab213524 anti PD L1 antibody EPR19759 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Anti-PD-L1 antibody [EPR19759] (ab213524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD-L1 with ab213524 at a dilution of 1 : 250. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue labelling PDI with ab243644 at 1.02 μg/mL (B), PD-L 1 with ab213524 at 1/100 dilution (C) and CD68 with ab213363 at 1/300 dilution (D). Anti-Rabbit and Mouse Polymer HRP was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Heat mediated antigen retrieval (Leica ER2, PH9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibodies from the previous round, to avoid any cross-reactivity.

Panel A : merged staining of anti- PD1 (green, Opal™520), anti- PD-L1 (red, Opal™570) and anti- CD68 (yellow, Opal™690).

Panel B : Anti- PD1 stained on antigen-stimulated T cells.

Panel C : anti- PD-L1 stained on cells involved in T cell inhibition

Panel D : anti-CD68 stained on macrophages.

The section was incubated in three rounds of staining : in the order of ab243644, ab213363 and ab213524 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR19759] (AB213524)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCI-H1975 (Human lung non small cell carcinoma cell line) cells labeling PD-L1 with ab213524 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing weakly membrane and cytoplasmic staining on NCI-H1975 cells.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab213524 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.

-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD-L1 with ab213524 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Membrane staining on the human tonsil crypt epithelium is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-PD-L1 antibody [EPR19759] (AB213524)

PD-L1 was immunoprecipitated from 0.35 mg of NCI-H1975 (Human non-small cell lung cancer cell line) whole cell lysate with ab213524 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab213524 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : NCI-H1975 whole cell lysate 10μg (Input).

Lane 2 : ab213524 IP in NCI-H1975 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213524 in NCI-H1975 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-PD-L1 antibody [EPR19759] (ab213524)

Predicted band size: 33 kDa

false

Lab

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

ab228415 works better than ab213524 in western blot testing. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results. Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-PD-L1 antibody [EPR19759] (ab213524) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 40-60 kDa

false

Exposure time: 100s

Lab

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Lanes 1- 6 : Merged signal (red and green). Green - ab213524 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab213524 was shown to react with PD-L1 in wild-type A549 treated with 100 ng/ml IFN gamma for 48 h cells in western blot. Loss of signal was observed when both treated and untreated knockout cell lines ab267054 ( treated and untreated knockout cell lysates ab256831) were used. Wild-type A549 treated with 100 ng/ml IFN gamma for 48 h and CD274 knockout A549 treated with 100 ng/ml IFN gamma for 48 h cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab213524 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Lane 1:

Wild-type A549 treated with 100 ng/ml IFN gamma (<a href='/en-us/products/proteins-peptides/recombinant-human-interferon-gamma-protein-active-ab259377'>ab259377</a>) for 48 h cell lysate at 20 µg

Lanes 2 and 6:

CD274 knockout A549 treated with 100 ng/ml IFN gamma (<a href='/en-us/products/proteins-peptides/recombinant-human-interferon-gamma-protein-active-ab259377'>ab259377</a>) for 48 h cell lysate at 20 µg

Lane 2:

Western blot - Human CD274 (PD-L1) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cd274-pd-l1-knockout-a549-cell-line-ab267054'>ab267054</a>)

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Lane 5:

Wild-type A549 untreated cell lysate at 20 µg

Predicted band size: 33 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Lanes 1 - 6 : Merged signal (red and green). Green - ab213524 observed at 50 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab213524 Recombinant Anti-PD-L1 antibody [EPR19759] was shown to specifically react with PD-L1 in wild-type A549 treated with 100 ng/mL IFN gamma for 48 h cells in western blot. Loss of signal was observed when both treated and untreated knockout cell line ab267055 (treated and untreated knockout cell lysates ab256866) were used. Wild-type and PD-L1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab213524 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PD-L1 antibody [EPR19759] (ab213524) at 1/1000 dilution

Lane 1:

Wild-type A549 treated with 100 ng/mL IFN gamma for 48 h cell lysate at 20 µg

Lane 2:

CD274 knockout A549 treated with 100 ng/mL IFN gamma for 48 h cell lysate at 20 µg

Lane 2:

Western blot - Human CD274 (PD-L1) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cd274-pd-l1-knockout-a549-cell-line-ab267055'>ab267055</a>)

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Lane 5:

Wild-type A549 untreated cell lysate at 20 µg

Lane 6:

CD274 knockout A549 untreated cell lysate at 20 µg

Predicted band size: 33 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PD-L1 antibody [EPR19759] (ab213524) at 0.5 µg/mL

Lane 1:

Untreated A549 (Human lung carcinoma epithelial cell) whole cell lysate

Lane 2:

A549 (Human lung carcinoma epithelial cell) treated with 100ng/ml Interferon gamma for 48 hours whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Expression of PD-L1 varied widely among the tumor cell lines

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-PD-L1 antibody [EPR19759] (ab213524) at 1/1000 dilution

Lane 1:

H1975 (Human non-small cell lung cancer epithelial cell) whole cell lysate at 20 µg

Lane 2:

NCI-H1299 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 9:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 10:

DU 145 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 11:

A375 (Human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 12:

MeWo (Human malignant melanoma fibroblast) whole cell lysate at 20 µg

Lane 13:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 14:

Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 15:

HepG2(Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 16:

BXPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 17:

PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 18:

NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 19:

SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 20 µg

Lane 20:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 40-60 kDa

true

Exposure time: 180s

Lab

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Lanes 1- 6 : Merged signal (red and green). Green - ab213524 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

All lanes:

Western blot - Anti-PD-L1 antibody [EPR19759] (ab213524) at 1/1000 dilution

Lane 1:

Wild-type A549 treated with 100 ng/ml IFN gamma (<a href='/en-us/products/proteins-peptides/recombinant-human-interferon-gamma-protein-active-ab259377'>ab259377</a>) for 48 h cell lysate at 20 µg

Lane 2:

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Lane 5:

Wild-type A549 untreated cell lysate at 20 µg

Lane 6:

CD274 knockout A549 untreated cell lysate at 20 µg

Predicted band size: 33 kDa

Observed band size: 50 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized CHO (Chinese hamster ovary cell line) cells labeling PD-L1 with ab213524 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane and cytoplasmic staining on CHO-PDL1 cells.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab213524 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.

-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Intracellular Flow Cytometry analysis of CHO-PD-L1 (red) and CHO-S (blue) cells labelling PD-L1 with ab213524 at 1/500. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% tween-20-PBS and blocked with 10% goat serum. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.

Supplier Data

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Blocking/Dilution buffer : 5% NFDM/TBST.

The lower band is predicted to be isoform 2.

All lanes:

Western blot - Anti-PD-L1 antibody [EPR19759] (ab213524) at 1/1000 dilution

Lane 1:

Chinese hamster ovary cell lysate at 10 µg

Lane 2:

Chinese hamster ovary cell lysate overexpressing PD-L1 at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa

Observed band size: 40-60 kDa

false

Exposure time: 3s

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PD-L1 antibody [EPR19759]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PD-L1 antibody [EPR19759]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PD-L1 antibody [EPR19759]
Carrier free

Anti-PD-L1 antibody [EPR19759] - BSA and Azide free
HRP

HRP Anti-PD-L1 antibody [EPR19759]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PD-L1 antibody [EPR19759]
Carrier free

Anti-PD-L1 antibody [EPR19759] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19759

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product.

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Reactivity data

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Product details

Anti-PD-L1 antibody [EPR19759] (ab213524) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human samples.

Anti-PD-L1 antibody [EPR19759] (ab213524) has been cited over 96 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-PD-L1 antibody [EPR19759] (ab213524) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-PD-L1 antibody [EPR19759] (ab213524) has been confirmed by testing in knockout samples.

Anti-PD-L1 antibody [EPR19759] (ab213524) specifically detects PD-L1 (UniProt ID: Q9NZQ7; Molecular weight: 31kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR19759 - ab221612.

Antibody clone EPR19759 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP, Alexa Fluor® 594, Alexa Fluor® 568 (ab214958, ab215251, ab215349, ab216742, ab22314).

Programmed death-ligand 1 (PD-L1) is a critical protein in immuno-oncology, playing a significant role in tumor immune evasion. It is expressed on the surface of tumor cells and binds to the PD-1 receptor on T cells, inhibiting their activity and allowing tumors to escape immune detection.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.

Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

See full target information CD274

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Publications (159)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1658679 PubMed40977688

2025

CRTC1 enhances PD-L1-mediated tumor immunosuppression in non-small cell lung cancer via the Notch1/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Feng,Yuan Shi,Fang Yuan,Yanxia Hu,Xiangdong Tang,Wei Zhang,Jiadi Gan,Longhua Sun,Lingling Cao

BMC cancer 25:1417 PubMed40898094

2025

KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Li,Qiang Feng,Ya Jiang,Lilin Yang,Hong Fang,Wenmang Xu,Yuanyuan Wang,Xinyan Pan,Julun Yang

Journal for immunotherapy of cancer 13: PubMed40789740

2025

Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatong Xiao,Jinhui Liu,Chunyu Zhang,Zhi Liu,Zhenyu Nie,Zhenglin Yi,Xin Gao,Haisu Liang,Jinliang Huang,Zhiyong Cai,Luzhe Yan,Bingquan Wu,Zefu Liu,Jinbo Chen,Xiongbing Zu,Jiao Hu

International journal of nanomedicine 20:8833-8859 PubMed40657481

2025

Photothermal Therapeutic Gold Nanoparticles Loaded with PD-L1 siRNA Enhanced Killing of NSCLC Cells by Immune Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gao,Li-Hua Yang,Fang-Cheng Jiang,Wang-Yang Ye,Xin Chen,Zhao-Ji Liu,Rong-Quan He,Wan-Ying Huang,Zhen-Bo Feng,Jin-Liang Kong,Ze-Feng Lai,Gang Chen

Journal of translational medicine 23:778 PubMed40640907

2025

USP5-Mediated PD-L1 deubiquitination regulates immunotherapy efficacy in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaheng Xie,Pengpeng Zhang,Yuankun Liu,Dan Wu,Xueqi Ou,Ming Wang,Wei Yan,Jianlan Liu,Jian Tang,Tianyi Ni,Chenfeng Ma,Qikai Tang,Songyun Zhao,Min Qi

Frontiers in immunology 16:1618487 PubMed40568593

2025

Prognostic and immunological implications of cathepsin Z overexpression in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junyue Tao,Yiding Chen,Xiaokang Bian,Tingting Cai,Changhao Song,Chaozhao Liang,Zongyao Hao,Jialing Meng,Qintao Ge,Jun Zhou

Blood cancer journal 15:108 PubMed40514360

2025

Early growth response 1 as a key regulator of PD-L1 expression and immune evasion in extranodal NK/T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Yun Lee,Kui-Jin Kim,Woochan Park,Jeongmin Seo,Minsu Kang,Eun Hee Jung,Sang-A Kim,Koung Jin Suh,Ji-Won Kim,Se Hyun Kim,Jeong-Ok Lee,Jin Won Kim,Yu Jung Kim,Keun-Wook Lee,Jee Hyun Kim,Soo-Mee Bang,Tae Min Kim,Jin Ho Paik

Materials today. Bio 32:101875 PubMed40496725

2025

A triple-targeting "nano-brake" remodeling the impaired immune microenvironment in skin lesions for psoriasis treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Chen,Baiqun Duan,Shize Li,Yixuan Zhu,Zihao Huang,Dingchao Shen,Zhanzheng Ye,Yuqi Yan,Chenyu Qiu,Haoxiong Guan,Yinsha Yao,Jie Dong,Fugen Wu,Xinyu Jiang,Xianbao Shi,Longfa Kou

Nature communications 16:5154 PubMed40461504

2025

Inhibition of tumor-intrinsic NAT10 enhances antitumor immunity by triggering type I interferon response via MYC/CDK2/DNMT1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Cheng Liu,Yi-Hong Wei,Jin-Feng Chen,Xiang-Ling Xing,He-Xiao Jia,Xin-Yu Yang,Ying-Jian Huang,Xiao-Min Liu,Ke Xiao,Xiao-Dong Guo,Can Can,A-Min Zhang,Na He,Hai-Lei Zhang,Dao-Xin Ma

The EMBO journal 44:3085-3120 PubMed40263598

2025

SDCBP/Syntenin-1 stabilizes BACH1 by disassembling the SCF-BACH1 complex in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Phi-Long Tran,Okhwa Kim,Cheol Hwangbo,Hyo-Jin Kim,Young-Myeong Kim,Jeong-Hyung Lee

View all publications

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Anti-PD-L1 antibody [EPR19759]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunoprecipitation - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR19759] (AB213524)

Flow Cytometry (Intracellular) - Anti-PD-L1 antibody [EPR19759] (AB213524)

Western blot - Anti-PD-L1 antibody [EPR19759] (AB213524)

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