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Anti-PD-L1 antibody [EPR20529] ab213480 is a rabbit monoclonal antibody that is used in PD-L1 western blotting and immunofluorescence. Suitable for mouse samples

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with Cd274 knockout cell line validation
- Antibody clone EPR20529 is cited in over 100 publications


Images

Western blot - Anti-PD-L1 antibody [EPR20529] (AB213480), expandable thumbnail
  • Western blot - Anti-PD-L1 antibody [EPR20529] (AB213480), expandable thumbnail
  • Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (AB213480), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (AB213480), expandable thumbnail
  • Western blot - Anti-PD-L1 antibody [EPR20529] (AB213480), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFIHC-Fr
Mouse
Tested
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11238124). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11238124). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:11015443, PubMed:12719480).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:12218188, PubMed:27281199). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:12218188). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:12218188).

Alternative names

Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, Pdcd1l1, Pdcd1lg1, Pdl1, B7h1, Cd274

Recommended products

Anti-PD-L1 antibody [EPR20529] ab213480 is a rabbit monoclonal antibody that is used in PD-L1 western blotting and immunofluorescence. Suitable for mouse samples

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with Cd274 knockout cell line validation
- Antibody clone EPR20529 is cited in over 100 publications

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20529

Purification technique

Affinity purification Protein A

Specificity

This antibody is not suitable in IHC-FR for mouse samples

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.

Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

Associated diseases and disorders

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480), expandable thumbnail

    Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)

    This WB was performed by using YCA-R20529(BF)-111A H3L2 YR120921PJ, 1:5000 dilution. Working concentration: 0.402 μg/ml.

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/5000 dilution

    Lane 1: Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: RAW264.7 treated with 100 ng/ml IFN gamma for 48 h, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 40-60 kDa

    Exposure time: 37s

  • Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480), expandable thumbnail
    This image is courtesy of an anonymous collaborator.

    Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)

    The mAb is specific for mouse mPDL1 and does not recognise hPDL1. As expected a specific 48kDa band is observed in RAW264.7 and MEFS cell extract after Interferon-gamma treatment. No bands are observed in RAW264.7 and MEFS WT and in MEFS KO with and without Interferon-gamma treatment.

    All lanes: Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480) at 100 µg

    Lane 1: HEK mPDL1 cell lysate at 20 µg

    Lane 2: HEK hPDL1 cell lysate at 20 µg

    Lane 3: HEK CL7 at 20 µg

    Lane 4: RAW 264.7 Wild-type (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 100 µg

    Lane 5: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate treated with Interferon-gamma at 100 µg

    Lane 6: MEF Wild-type (Mouse embryonic fibroblast cell line) whole cell lysate at 100 µg

    Lane 7: MEF (Mouse embryonic fibroblast cell line) whole cell lysate treated with Interferon-gamma at 100 µg

    Lane 8: MEF mPDL1 KO cell lysate at 100 µg

    Lane 9: MEF mPDL1 KO treated with Interferon-gamma at 100 µg

    Predicted band size: 33 kDa

    Observed band size: 48 kDa

    Exposure time: 5min

  • Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480), expandable thumbnail

    Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)

    PD-L1 was immunoprecipitated from 0.35 mg of Mouse placenta lysate with ab213480 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213480 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1,000 dilution.

    Lane 1: Mouse placenta lysate, 10 μg (Input).

    Lane 2: ab213480 IP in mouse placenta lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213480 in mouse placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)

    Predicted band size: 33 kDa

    Observed band size: 55-60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)

    ab213480 staining PD-L1 in RAW264.7 from Mouse macrophage cell line transformed with Abelson murine leukemia virus by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% Methanol. Samples were incubated with ab213480 at 0.4 μg/ml. AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab196889) was used as counterstain at 2.5 μg/ml. DAPI used as nuclear counterstain. Confocal image showing membranous staining on RAW 264.7 cells treated with IFN-γ (100 ng/ml) for 24 hours.

  • Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480), expandable thumbnail

    Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature. PMID: 15353579.

    All lanes: Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/1000 dilution

    All lanes: Mouse placenta lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 40-60 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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