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Rabbit Recombinant Monoclonal PD-L2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-PD-L2 antibody [CAL28] (AB256386), expandable thumbnail
  • Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (AB256386), expandable thumbnail
  • Western blot - Anti-PD-L2 antibody [CAL28] (AB256386), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] (AB256386), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Although this clone performed well in IHC-P on tonsil tissue, non-specific nuclear staining was seen on TMA. The staining pattern did not correlate with ISH, so we cannot recommend this clone for IHC.

Both EDTA buffer pH9 and citrate buffer pH6 have been used successfully. Please refer to figure legends.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

2 products for Alternative Version

Target data

Function

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).

Alternative names

CD273, B7DC, PDCD1L2, PDL2, PDCD1LG2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC

Recommended products

Rabbit Recombinant Monoclonal PD-L2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
CAL28
Purification technique
Affinity purification Protein A
Specificity

Although this clone performed well in IHC-P on tonsil tissue, non-specific nuclear staining was seen on TMA. The staining pattern did not correlate with ISH, so we cannot recommend this clone for IHC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PD-L2 also known as PDCD1LG2 or B7-DC is a member of the B7 protein family that plays a role in immune system regulation. This protein has a molecular mass of approximately 25 kDa. PD-L2 is expressed in antigen-presenting cells such as dendritic cells and macrophages. Expression is also located in some subsets of T cells and B cells. The PD-L2 can act as a second ligand for the PD-1 receptor which is important in immune checkpoints.

Biological function summary

PD-L2 offers protection to tissues from autoimmunity by regulating T-cell activation and fostering tolerance. By engaging with its receptor PD-1 PD-L2 forms a complex that modulates the immune response to prevent overactivation. This interaction limits the immune system's potential damage to healthy tissues maintaining proper immune function in diverse environments.

Pathways

PD-L2 participates in the immune checkpoint pathways where it works closely with PD-1 to mediate immune responses. PD-L2 forms an important part of the program death-1 (PD-1) pathway which plays a prominent role in downregulating T-cell activity. It relates to proteins such as PD-L1 which also interacts with PD-1 modulating immune tolerance and response to infections or tumors.

Associated diseases and disorders

PD-L2 involvement has been significant in cancer immunology and autoimmune conditions. Its interaction with PD-1 relates to the suppression of immune responses in tumors contributing to cancer progression. PD-L2's function as an immune checkpoint ligand associates with autoimmune diseases like rheumatoid arthritis where immune regulation goes awry. Both PD-1 and PD-L2 represent potential targets for therapeutic interventions due to their roles in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PD-L2 antibody [CAL28] (ab256386), expandable thumbnail

    Western blot - Anti-PD-L2 antibody [CAL28] (ab256386)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Negative control: Jurkat. (PMID: 27631416).

    All lanes: Western blot - Anti-PD-L2 antibody [CAL28] (ab256386) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa

    Observed band size: 45-60 kDa

  • Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (ab256386), expandable thumbnail

    Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (ab256386)

    PD-L2 was immunoprecipitated from 0.35 mg of HDLM-2 (human Hodgkin lymphoma) whole cell lysate with ab256386 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab256386X at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.


    Lane 1: HDLM-2 whole cell lysate 10 μ (Input).
    Lane 2: ab256386 IP in HDML-2 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab256386 in HDML-2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

    All lanes: Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (ab256386)

    Predicted band size: 31 kDa

    Observed band size: 45-60 kDa

  • Western blot - Anti-PD-L2 antibody [CAL28] (ab256386), expandable thumbnail

    Western blot - Anti-PD-L2 antibody [CAL28] (ab256386)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-PD-L2 antibody [CAL28] (ab256386) at 1/1000 dilution

    Lane 1: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg

    Lane 3: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

    Lane 4: Saos-2 (human osteosarcoma cell line) whole cell lysate at 20 µg

    Lane 5: Human tonsil lysate at 20 µg

    Lane 6: Human lung lysate at 20 µg

    Lane 7: Human lung cancer lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa

    Observed band size: 45-60 kDa

  • Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] (ab256386), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] (ab256386)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HDLM-2 (human Hodgkin lymphoma) cells labeling PD-L2 with ab256386 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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